Development and Assessment of a Single Tube Internally Controlled Multiplex PCR Assay to Detect Different Pathogenic Bacteria Involved in Blood Stream Infections

Arabestani, Mohammad Reza; Fazzeli, Hossein; Esfahani, Bahram Nasr; Alikhani, Mohammad Yousef

doi:10.17795/ijep10601

Cited by 1 publication

(1 citation statement)

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“…Interpretation of obtained results of new NATs associated with BCs for diagnosis of BSI is challenging when a positive result for bacterial or fungal nucleic acids is compared with a negative result for culture. [50] The reason for this contrary may be association with detection of pathogens as a result of a higher sensitivity of NAT for certain slow-growing or fastidious organisms, or associated with circulating nucleic acids or nonproliferating, nonviable or degraded pathogens, which may be lack of clinical importance.…”

Section: Identification By Microbial Nucleic-acid Technologymentioning

confidence: 99%

Conventional, molecular methods and biomarkers molecules in detection of septicemia

et al. 2015

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Sepsis is a leading cause of morbidity and mortality in hospitalized patients worldwide and based on studies, 30–40% of all cases of severe sepsis and septic shock results from the blood stream infections (BSIs). Identifying of the disease, performing laboratory tests, and consequently treatment are factors that required for optimum management of BSIs. In addition, applying precise and immediate identification of the etiologic agent is a prerequisite for specific antibiotic therapy of pathogen and thereby decreasing mortality rates. The diagnosis of sepsis is difficult because clinical signs of sepsis often overlap with other noninfectious cases of systemic inflammation. BSIs are usually diagnosed by performing a series of techniques such as blood cultures, polymerase chain reaction-based methods, and biomarkers of sepsis. Extremely time-consuming even to take up to several days is a major limitation of conventional methods. In addition, yielding false-negative results due to fastidious and slow-growing microorganisms and also in case of antibiotic pretreated samples are other limitations. In comparison, molecular methods are capable of examining a blood sample obtained from suspicious patient with BSI and gave the all required information to prescribing antimicrobial therapy for detected bacterial or fungal infections immediately. Because of an emergency of sepsis, new methods are being developed. In this review, we discussed about the most important sepsis diagnostic methods and numbered the advantage and disadvantage of the methods in detail.

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Section: Identification By Microbial Nucleic-acid Technologymentioning

confidence: 99%