Development and Application of Time-Resolved Surface Plasmon Resonance Spectrometer

Abstract

Surface plasmon resonance (SPR) sensor, an optical sensor exploiting special electromagnetic waves-surface plasmon polaritons to probe interactions between an analyte in solution and a molecular recognition element immobilized on the SPR sensor surface, has been widely used in various realms, such as investigating biomolecular interactions and binding properties, detection of biological and chemical analytes, environmental monitoring, food safety and medical diagnostics. This paper reviews the development of SPR sensors and SPR commercial instruments, and emphatically introduces the time-resolved surface plasmon resonance (TR-SPR) techniques. The excellent performances of high sensitivity and rapid detection are easily achieved with TR-SPR spectrometer, whereas the traditional SPR spectrometer cannot be accomplished. Therefore, TR-SPR spectrometer is appropriate for real time analysis of bio-recognition events and small molecular dynamics. However, only two commercial TR-SPR instruments have been exhibited at present, Thermo FT-SPR 100 and Autolab ESPRIT (SPRINGLE). Unfortunately, the high-priced instruments are not suitable for extensive applications in laboratories at present. Herein, a novel commercial TR-SPR spectrometer has been introduced in this review.

On The Application of SiO2/SiC Grating on Ag for High-Performance Fiber Optic Plasmonic Sensing of Cortisol Concentration

“…In the angular interrogation method, in order to measure the angular shift of the monochromatic beam, a position-sensitive detector (PSD) was used. At present, 0.001 • was the smallest angular shift that can be actually detected [36].…”

“…Here, the change in α SPR corresponding to a small change in C c is taken into consideration and 0.001 • is the smallest angular shift that can be detected [36]. Under the intensity interrogation method (IIM),…”

“…In AIM, we could determine the LOD as well as FOM (as per Equations (4) and (5)) while in IIM, the LOD and sensitivity (SI) could be chosen as performance evaluation criteria. The variation of LOD with cortisol concentration was shown in Figure 4a under AIM (Equation (5)) assuming that the smallest angular shift that can be detected was 0.001° [36].…”

Fluoride Fiber-Based Plasmonic Biosensor with Two-Dimensional Material Heterostructures: Enhancement of Overall Figure-of-Merit via Optimization of Radiation Damping in Near Infrared Region

“…The shift in position of resonance angle can be observed at the detector side. The minimum possible angular shift that can be detected is 0.001° [39] Furthermore, the power loss (dB) measurement at resonance can be performed using an optical power meter, which can be used for calculation of power loss ratio (PLR).…”

“…Therefore, the simulation results and the conclusions of the proposed study may vary if there is any variation (for any reason) in the bacterial phase of the tissue. Also, the tuning of angle (α) should be performed carefully and with as fine a resolution as possible (e.g., 0.001° [39]).…”