Development and Application of Tandem LC-MS Method for the Determination of Paralytic Shellfish Poisoning Toxins for Pollution Monitoring

Zhang, Xiaoling; Feng, Bing; Cai, You Qiong; Yu, Hao

doi:10.4028/www.scientific.net/amm.55-57.51

Cited by 3 publications

(3 citation statements)

References 11 publications

(9 reference statements)

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“…Cultures were kept at 25°C and a 12 h light:12 h dark cycle. LC-MS-MS analysis of the STX toxin production of the isolated microbial strain was performed according to the method reoprted previously (LC-MS-MS analysis of the STX toxin production of the isolated microbial strain was performed according to the method reoprted previously (Steidinger KA et al 2013, Zhang XL et al 2011). Molecular analysis of the sxtA biosynthesisi gene was performed according to the method reoprted previously (Steidinger KA et al 1993).…”

Section: Methodsmentioning

confidence: 99%

Biodiversity Study of Intracellular Bacteria Closely Associated with Paralytic Shellfish Poisoning Dinoflagellates Alexandrium tamarense and A. minutum

Ma¹,

Tian²,

Huang³

et al. 2015

International Journal of Environment and Resource

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Paralytic shellfish poisoning (PSP) toxins are potent environmental neurotoxins mainly produced by marien dinoflagellates. Alexandrium spp. are the most abundant and widespread producer of saxitoxin (STX). Currently, the evolutionary history that resulted in the curious cross-kingdom distribution of STX-synthesis remained unclear. However, the increasing number of findings of toxin-producing bacteria in dinoflagellate supports the hypothesis of the bacterial origin of PSP toxins. Addtionally, evidence that there are specific symbiosis bacterial taxa associated with phytoplankton indicates the presence of specific selective mechanisms between them, and implies that the symbiosis bacteria have some vital functions to the benefit of the dinoflagellates. Culture-independent high-throughput pyrosequencing analysis is able to provide a thorough description of microbiota community in environmental samples, which also helps to reveal their potential function. This paper aims to demonstrate the biodiversity of the bacteria closely associated with two toxic dinoflagellate strains, A. tamarense and A. minutum using the pyrosequencing method.

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Section: Methodsmentioning

confidence: 99%

Biodiversity Study of Intracellular Bacteria Closely Associated with Paralytic Shellfish Poisoning Dinoflagellates Alexandrium tamarense and A. minutum

Ma¹,

Tian²,

Huang³

et al. 2015

International Journal of Environment and Resource

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“…Algal toxin extraction was performed using acetic acid and ultrasound. The instrumental conditions were determined with reference to the method of Zhang et al [54].…”

Section: Toxicity Type and Content Testmentioning

confidence: 99%

Screening of associated bacteria ofAlexandrium catenellaon affecting PSTs production and influence mechanism

Zou,

Yu,

Sun

et al. 2024

Preprint

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Alexandriumcauses serious food safety and human death due to paralytic shellfish toxins (PSTs) production. The associated bacteria can affect PSTs production ofAlexandrium. However, the influencing mechanism is still unclear. Here we firstly screened functional associated bacteria for affecting PSTs production ofAlexandrium catenellain Yangtze Estuary and further studied their influence on physiological process and molecular regulation ofA. catenella. Thirteen bacteria strains for affecting PSTs production ofA. catenellawere selected. TheA. catenellastrains co-cultured with different functional associated bacteria all produced more PSTs than axenic strain with antibiotic treatment. Compared with axenicA. catenella, the non-axenicA. catenellaproduced more algal cells, soluble sugar, soluble protein and neutral lipid. By RNA-seq, it was found that non-axenicA. catenellaproduced more upregulated functional genes than axenicA. catenella. The biosynthesis of cofactors and spliceosome were the dominant different pathways between axenic and non-axenicA. catenellastrains. The sxtA expression was closely related with Arginine and proline metabolism, Arginine biosynthesis, Fatty acid biosynthesis, TCA cycle and Glutathione metabolism, which were all downregulated in axenicA. catenella. Meantime, the non-axenicA. catenellaunder nitrogen deprivation produced less PSTs and functional genes than non-axenic strain under common culture condition, indicating the nitrogen significance for PSTs production. The detailed signal molecular released by associated bacteria for regulating PSTs ofA. catenellaneeds to be further studied.

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“…LC-MS analysis of the STX toxin production of the isolated microbial strain was performed according to the method reoprted previously LC-MS analysis of the STX toxin production of the isolated microbial strain was performed according to the method reoprted previously [8,14].…”

Section: Psp Analysis By Lc-msmentioning

confidence: 99%

Screening Assay Establishment for Toxigenic Bacteria from PSP-producing Dinoflagellate Alexandrium tamarense Cultures

Zhang

2016

Proceedings of the 2015 4th International Conference on Sustainable Energy and Environmental Engineering

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Paralytic shellfish poisoning (PSP) toxins are potent neurotoxins mainly produced by marine dinoflagellates. However, the increasing number of findings of toxin-producing bacteria in dinoflagellate intracellular cells supports the hypothesis of the bacterial origin of PSP toxins. Although toxigenic bacteria could be isolated from toxic dinoflagellates, it was not clearly proven whether the isolated bacterial strains based on culture-dependent manner and the corresponding intracellular bacteria were the same because of microbial unculturability and shorting of the genetic evidence. This paper aims to demonstrate the development of a screening assay for toxigenic bacteria from marine dinoflagellate A. tamarense based on molecular analysis of the possible sxtA gene for the toxic PSP biosynthesis.

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Development and Application of Tandem LC-MS Method for the Determination of Paralytic Shellfish Poisoning Toxins for Pollution Monitoring

Cited by 3 publications

References 11 publications

Biodiversity Study of Intracellular Bacteria Closely Associated with Paralytic Shellfish Poisoning Dinoflagellates Alexandrium tamarense and A. minutum

Biodiversity Study of Intracellular Bacteria Closely Associated with Paralytic Shellfish Poisoning Dinoflagellates Alexandrium tamarense and A. minutum

Screening of associated bacteria ofAlexandrium catenellaon affecting PSTs production and influence mechanism

Screening Assay Establishment for Toxigenic Bacteria from PSP-producing Dinoflagellate Alexandrium tamarense Cultures

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