Development and application of spectrophotometric method for the determination of cefaclor in pharmaceutical formulations

Abstract

Recebido em 18/5/08; aceito em 13/1/09; publicado na web em 28/5/09 A simple, fast and sensitive spectrophotometric method for the determination of cefaclor in pharmaceutical raw and dosage forms based on reaction with ninhydrin is developed, optimized and validated. The purple color (Ruhemenn's purple) that resulted from the reaction was stabilized and measured at 560 nm. Beer's law is obeyed in the concentration range of 4-80 µg mL -1 with molar absorptivity of 1.42 × 10 5 L mole -1 cm -1 . All variables including the reagent concentration, heating time, reaction temperature, color stability period, and cefaclor/ninhydrin ratio were studied in order to optimize the reaction conditions. No interference was observed from common pharmaceutical adjuvant. The developed method is easy to use, accurate and highly cost-effective for routine studies relative to HPLC and other techniques.

Ninhydrin Based Visible Spectrophotometric Determination of Gemigliptin

“…For example, lmax was in the range of 595-600 nm when the medium was N,N'-dimethylformamide (DMF) [20][21][22][23][24][25] . In buffer solution or aqueous medium, 555 -571 nm was observed 14,15,[26][27][28][29][30][31] .This may be due to polar and stearic effects of reactant 32 .The observed l max can be further substantiated from the studies of Mendel Friedman et al, 33 about the effect of solvent nature on absorption maximum of Ruhemann's purple. According them, l max will be around 605 nm in non-aqueous aprotic solvents (like DMSO and DMF) and a hypsochromic shift was observed to 575 nm in non-aqueous protic solvents (formamide) or by the addition of protic solvents to aprotic media due to involvement of hydrogen bonding.…”