Development and application of polymerase chain reaction (PCR) tests for the detection of subgroup J avian leukosis virus

Smith, Lorraine P.; Brown, Susan; Howes, K.; McLeod, Scott; Arshad, Siti Suri; Barron, Gerard; Venugopal, K.; McKay, J. C.; Payne, L. N.

doi:10.1016/s0168-1702(98)00022-7

Cited by 159 publications

(128 citation statements)

References 14 publications

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“…The negative control used was DNA from uninoculated CEF. The primer sequences were prepared as follows: a 0.7-kb fragment corresponding to the 3' end of the pol and 5' part of the env regions was obtained by PCR using a primer set, H5 (5'-GGA TGA GGT GAC TAA GAA AG-3') and H2 (5'-ACT GGT GAA TCC ACA ATA TCT ACG-3') [20] from the brain tissue of a bantam affected with fowl glioma and cloned into pGEM-T vector (Promega Corp., Madison, WI, U.S.A.). The resultant fragment was sequenced using an ABI PRISM 310 Genetic Analyzer (Perkin-Elmer Applied Biosystems Inc., Foster City, CA, U.S.A.).…”

Section: Chicken and Inoculummentioning

confidence: 99%

Nonsuppurative Myocarditis Associated with So-Called Fowl Glioma.

et al. 2002

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ABSTRACT. C/O specific pathogen-free White Leghorn chickens were intracerebrally inoculated at one day of age with a brain homogenate of Japanese bantams (Gallus gallus domesticus) affected with fowl glioma. Histologically, six of eight inoculated chickens developed nonsuppurative meningoencephalitis in cerebrum and two of them had the characteristic lesions of fowl glioma. Hyperplastic lymphoid foci concomitantly developed in many organs of these birds, especially in the heart. Apart from these lymphoid foci, lymphocytic myocarditis was observed in all inoculated birds. Matrix inclusions were also noted in myocardial cells. Immunohistochemically, avian leukosis virus antigens were detected in reticular cells in the lymphoid foci, mesangial cells of the kidney, smooth muscle cells of the blood vessels, and myocardial cells. Of these tissues, the myocardium of all inoculated birds consistently showed strong reactivity for this antigens. The matrix inclusions were also positive for the antigens. These results suggest that the causal virus of fowl glioma has a high propensity to replicate, especially in myocardium and nonsuppurative myocarditis occurs associated with so-called fowl glioma. KEY WORDS: avian leukosis virus, chicken, fowl glioma, myocarditis.

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Section: Chicken and Inoculummentioning

confidence: 99%

Nonsuppurative Myocarditis Associated with So-Called Fowl Glioma.

et al. 2002

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“…Analysis of PCR assays with ALV-J specific primers (Smith et al, 1998) revealed positive reaction with DNA obtained from tumors, indicating that the viral particles which were observed in transmission electron microscopy were ALV-J.…”

Section: Discussionmentioning

confidence: 98%

“…The sequences of the oligonucleotide primers (H5 and H7) used in the study were selected from the published sequences (Smith et al, 1998). Primers were obtained from Life Technologies 2 .…”

Section: Methodsmentioning

confidence: 99%

Histopathological and ultrastructural characteristics of myeloid leukosis in broiler chicken

Manzan

Baccaro

Ferreira

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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An ultrastructural and histological study was performed to determine the degree of differentiation of the neoplastic cells. The histological study revealed neoplastic cells with pleomorphism, oval nuclei, prominent nucleoli, irregularly distributed chromatin, atypical mitotic figures and moderate amount of cytoplasm containing spherical eosinophilic granulations, typical features of the myeloid lineage. Ultrastructurally, there were cells with an electron-dense, oval and voluminous nucleus, with predominant euchromatin and cytoplasm containing many spherical, electron-dense and homogeneous granules, indicative of myelocytes with differentiation to eosinophils. Type-C viral particles were also seen in the intercellular space of renal tubules and inside the intracytoplasmic vesicles of immature myelocytes in the bone marrow and ovary. PCR was positive to ALV-J.Keywords: chicken, avian leukosis, avian oncovirus, histopathology, ultrastructure RESUMO Caracterizaram-se a linhagem e o grau de diferenciação das células neoplásicas no estudo

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“…The extraction of RNA from frozen livers was performed using TRIzol Reagent (Gibco-BRL Life Technologies, U.S.A.) according to the manufacturer's instructions. RT-PCR based on specific primers H5 (5'-GGA TGA GGT GAC TAA GAA AG-3') and H7 (5'-CGA ACC AAA GGT AAC ACA CG-3') [8] of subgroup J ALV was performed using a commercial kit (Ready-To-Go RT-PCR Beads, Amersham Pharmacia Biotech, U.S.A.). A mixture (50 µl) containing 46 or 47 µl of diethylpyrocarbonate-treated water (Bio 101, Inc., U.S.A.), 1 µl of each primer and 1 or 2 µl of template RNA was added to the reagents for reverse transcription in the kit.…”

Section: Reverse Transcriptase-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Histopathological Characteristics of Spindle-cell Proliferative Disease in Broiler Chickens and Its Experimental Reproduction in Specific Pathogen-Free Chickens

Takami

Goryo

Masegi

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The livers and spleens of 45 broiler chickens (33 to 79 days old) suspected of Marek's disease (MD) at meat inspection were collected and examined histopathologically. Macroscopically, they were enlarged from two to three times, and multiple, small, white areas of plaque or infrequent, large, white nodules were observed in most cases. Only 9 birds (20%) were diagnosed with MD based on the histological examination, while the other 35 birds (78%) had tumor-like proliferative lesions in the Glisson's sheath of the liver and in the white pulp and around the sheathed arteries of the spleen, which differs from the pattern seen in MD. The proliferating cells were mainly spindle-shaped or pleomorphic, and were variable in size with abundant eosinophilic cytoplasm. The disease giving r ise to the present lesions was diagnosed tentatively as spindle-cell proliferative disease. Total 50 1-day-old specific pathogen-free chicks by serial passage were inoculated intramuscularly with 0.1 m l of a 10% homogenate of the affected livers or spleens. Microscopically, one inoculated bird, necropsied at 6 weeks of age, had spindle-cell proliferative lesions in the spleen similar to the lesions of na turally occurring spindle-cell proliferative disease. Some birds had tumorous lesions, including renal adenoma, leiomyosarcoma and myxosarcoma. Reverse transcriptase-polymerase chain reaction performed using primers specific for subgroup J avian leukosis virus (ALV) produced specific amplifications of subgroup J ALV genes in 4 of 5 field cases examined. KEY WORDS: broiler chicken, Marek's disease, spindle-cell, subgroup J avian leukosis virus, tumor-like proliferative lesion.

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Development and application of polymerase chain reaction (PCR) tests for the detection of subgroup J avian leukosis virus

Cited by 159 publications

References 14 publications

Nonsuppurative Myocarditis Associated with So-Called Fowl Glioma.

Nonsuppurative Myocarditis Associated with So-Called Fowl Glioma.

Histopathological and ultrastructural characteristics of myeloid leukosis in broiler chicken

Histopathological Characteristics of Spindle-cell Proliferative Disease in Broiler Chickens and Its Experimental Reproduction in Specific Pathogen-Free Chickens

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