Development and application of new nucleic acid-based technologies for microbial community analyses in foods

Rudi, Knut; Nogva, Hege Karin; Moen, Birgitte; Nissen, Hilde; Bredholt, Sylvia; Møretrø, Trond; Naterstad, Kristine; Holck, Askild Lorentz

doi:10.1016/s0168-1605(02)00236-2

Cited by 54 publications

(34 citation statements)

References 32 publications

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“…The combination of rapidity, good sensitivityand specificity, and ease of performance has made PCR technology an appealing alternative to culture-based and immunological-based methods for pathogen detection in foods. [7], diagnosis of the causative agent of diarrheas cannot depend only on the clinical features of the patients but requires proper diagnosis of the infectious agent in the laboratory [8].…”

Section: Introductionmentioning

confidence: 99%

Detection of Escherichia coli O157:H7 in Human patients Stool and Food by Using Multiplex PCR Assays Targeting the rfbE and the eaeAGenes compared with Detection by Biochemical Test and Serological Assay

Al-Dragy

Baqer

2017

JNUS

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Escherichia coli O157:H7 is an important food borne pathogen has been linked to foods of bovine origin and fresh produce. Rapid and sensitive identification of this dangerous pathogen is important for patient management and for prompt epidemiological investigations.This study aimed to detect Escherichia coli O157:H7 presence in samples from diarrhea patients, and food (imported beef and lettuce) and characterize by selective media, biochemical tests, latex agglutination test, and compared with PCR technique. during the period from September 2012 to February 2013 A total 120 samples were collected 50 from diarrhea patients and 70 food samples including (45 imported beef and 25 lettuce) All samples were screened to detect the presence of non-sorbitol fermenting colonies (NSF) on sorbitol Mac Conkey agar supplemented with Cefixime (C-SMAC). A total of 120 isolates, 22 (44%) from diarrhea patients, 18 (40%) from meat and 9 (36%) from lettuce were non-sorbitol fermenting, E. coli isolates were serotyped as E. coli O157:H7 by latex agglutination test, 10(20%) isolates of diarrhea patients, 7 (15.5%) isolates of meat and 5 (20%) isolates of lettuce, Results of the genetic diagnosis using polymerase chain reaction (PCR)with specific primers (eaeA and rfbE genes) by MPCR revealed that 10(20%) isolates of diarrhea patients5 (11.1%) isolate from meat and 5(20 %) of lettuce samples were positive.PCR has become a very rapid and reliable tool for the molecular diagnosis of E. coli O157.

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Section: Introductionmentioning

confidence: 99%

Detection of Escherichia coli O157:H7 in Human patients Stool and Food by Using Multiplex PCR Assays Targeting the rfbE and the eaeAGenes compared with Detection by Biochemical Test and Serological Assay

Al-Dragy

Baqer

2017

JNUS

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“…To discriminate live and dead bacteria by PCR, cross-linking agents such as psoralen, a methylisopsoralen derivative (4Ј-aminomethyl-4,5Ј-dimethylisopsoralen[4Ј-AMDMIP]), and ethidium monoazide (EMA) have been used (4,5,20,23,25,(27)(28)(29). They selectively permeate the cell walls of dead bacteria and irreversibly bind to chromosomal DNA by covalent attachment (20,23,(27)(28)(29).…”

mentioning

confidence: 99%

“…They selectively permeate the cell walls of dead bacteria and irreversibly bind to chromosomal DNA by covalent attachment (20,23,(27)(28)(29). It has been reported that EMA could cross-link to DNA at the rate of 1 agent per 10 to 80 bp in vitro (17).…”

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confidence: 99%

Method To Detect Only Live Bacteria during PCR Amplification

et al. 2008

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“…Although cultures and serologic tests are widely used to identify Brucella agents in tissue, waste, blood and food, the PCR technique has also become more prevalent in recent years (Gupta et Rudi et al, 2002). This technique can be used with specific enzymes to identify a specific genus (Tantillo et al, 2001;Funk et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

The Detection of Brucella Bacteria with PCR and Bacteriological Method in Raw Milk and Some of the Dairy Products Which Are Consumed in Kars

Gülbaz¹,

Kamber²

2016

BUASVMCN-VM

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Because of the abort by the contamination to the humans via direct contact with infected animals or the consumption of contaminated raw milk and dairy products, brucellosis greatly causes to economic loss. For this reason, the annihilating of the effect of this disease is considerably important. Here, we aimed to detect the existence of Brucella bacteria in raw milk, cheese which is produced from raw milk and butter samples by using isolation, identification and PCR molecular techniques. Brucella type agents were detected in within a year by using 315 samples; 215 raw milk, 50 cheeses, 50 butter. Cheese and butter samples were obtained from raw milk. The grams coloring, oxidase, catalase, urease and H 2 S analysis of 215 raw milk samples showed that only 4 (1.86%) samples was Brucella positive. As a result, we have determined that there are several types of Brucellosis bacteria in milk, cheese and butter samples. Milk and dairy products can create a risk in Kars because of brucellosis.

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Development and application of new nucleic acid-based technologies for microbial community analyses in foods

Cited by 54 publications

References 32 publications

Detection of Escherichia coli O157:H7 in Human patients Stool and Food by Using Multiplex PCR Assays Targeting the rfbE and the eaeAGenes compared with Detection by Biochemical Test and Serological Assay

Detection of Escherichia coli O157:H7 in Human patients Stool and Food by Using Multiplex PCR Assays Targeting the rfbE and the eaeAGenes compared with Detection by Biochemical Test and Serological Assay

Method To Detect Only Live Bacteria during PCR Amplification

The Detection of Brucella Bacteria with PCR and Bacteriological Method in Raw Milk and Some of the Dairy Products Which Are Consumed in Kars

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