Development and application of liquid and gas-chromatographic speciation techniques with element specific (ICP-MS) detection to the study of anaerobic arsenic metabolism

Wickenheiser, E. B.; Michalke, Klaus; Drescher, C.; Hirner, Alfred V.; Hensel, Reinhard

doi:10.1007/s002160051114

Cited by 31 publications

(18 citation statements)

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“…Pseudomonas and Alcaligenes also produced AsH 3 from both As(III) and As(V) under anaerobic conditions (Cheng and Focht, 1979). According to Wickenheiser's research, TMAs is the only volatile As species formed by sulfatereducing bacteria and the peptolytic bacteria Clostridium collagenovorans, Desulfovibrio gigas and D. vulgaris (Wickenheiser et al, 1998;Michalke et al, 2000). Though TMAs is prone to be oxidized to nonvolatile TMAsO in water environment, TMAsO can be reduced de novo under aerobic or anaerobic conditions by several bacteria.…”

Section: Bacteriamentioning

confidence: 99%

A review on completing arsenic biogeochemical cycle: Microbial volatilization of arsines in environment

Wang¹,

Sun²,

Jia³

et al. 2014

Journal of Environmental Sciences

146

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a b s t r a c t Arsenic (As) is ubiquitous in the environment in the carcinogenic inorganic forms, posing risks to human health in many parts of the world. Many microorganisms have evolved a series of mechanisms to cope with inorganic arsenic in their growth media such as transforming As compounds into volatile derivatives. Bio-volatilization of As has been suggested to play an important role in global As biogeochemical cycling, and can also be explored as a potential method for arsenic bioremediation. This review aims to provide an overview of the quality and quantity of As volatilization by fungi, bacteria, microalga and protozoans. Arsenic bio-volatilization is influenced by both biotic and abiotic factors that can be manipulated/elucidated for the purpose of As bioremediation. Since As biovolatilization is a resurgent topic for both biogeochemistry and environmental health, our review serves as a concept paper for future research directions.

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Section: Bacteriamentioning

confidence: 99%

A review on completing arsenic biogeochemical cycle: Microbial volatilization of arsines in environment

Wang¹,

Sun²,

Jia³

et al. 2014

Journal of Environmental Sciences

146

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“…The metal and metalloid content of the sewage sludge was determined by X-ray fluorescence (XRF) analysis with a Spectro XLAB 2000 as described elsewhere (34). Volatile metal(loid) compounds in the headspace of the samples were analyzed, as described previously (39), using a modified purgeand-trap gas chromatographic system coupled to an inductively coupled plasma mass spectrometer (ICP-MS; Fisons VG PlasmaQuad II) as an element specific detector. The volatile metal(loid) compounds were identified by boiling point retention time correlation (R ϭ 0.998) and by comparison with the retention times of standards of trimethylarsines (TMAs) and TMBi, as well as those of arsine (AsH 3 ), monomethylarsine (MMAs), and dimethylarsine (DMAs) produced by reaction of sodium borohydride with inorganic arsenic, monomethylarsenous acid, and cacodylic acid, respectively (40).…”

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confidence: 99%

Production of Volatile Derivatives of Metal(loid)s by Microflora Involved in Anaerobic Digestion of Sewage Sludge

Michalke¹,

Wickenheiser

Mehring³

et al. 2000

Appl Environ Microbiol

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190

136

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Gases released from anaerobic wastewater treatment facilities contain considerable amounts of volatile methyl and hydride derivatives of metals and metalloids, such as arsine (AsH 3 ), monomethylarsine, dimethylarsine, trimethylarsine, trimethylbismuth (TMBi), elemental mercury (Hg 0 ), trimethylstibine, dimethyltellurium, and tetramethyltin. Most of these compounds could be shown to be produced by pure cultures of microorganisms which are representatives of the anaerobic sewage sludge microflora, i.e., methanogenic archaea (Methanobacterium formicicum, Methanosarcina barkeri, Methanobacterium thermoautotrophicum), sulfate-reducing bacteria (Desulfovibrio vulgaris, D. gigas), and a peptolytic bacterium (Clostridium collagenovorans). Additionally, dimethylselenium and dimethyldiselenium could be detected in the headspace of most of the pure cultures. This is the first report of the production of TMBi, stibine, monomethylstibine, and dimethylstibine by a pure culture of M. formicicum.Volatile methyl and hydride derivatives of metal(loid)s are found in gases released from natural environments such as sediments, wetlands (40), and hydrothermal springs (26), as well as from anthropogenic environments such as wastewater treatment plants and waste deposits (18). Some of the compounds are of anthropogenic origin, for example, alkylated tin and lead derivatives, whereas others are produced in environmental settings either due to chemical transalkylation or due to biologically mediated methylation and hydride formation (the addition of a formal hydride ion mediated by an organism) (11,36). The production of volatile metal(loid) compounds is a significant part of the biogeochemical cycles of metals (e.g., Bi, Hg, and Sn) and metalloids (e.g., As, Sb, Se, and Te) because of the increased mobility of the resultant compounds. Additionally, most of the volatile derivatives exhibit higher toxicity than their inorganic counterparts, since organic derivatives are lipophilic and are thus more biologically active (35,36). Exceptions are arsenic and selenium, in which cases the inorganic, nonmethylated forms are more toxic than the methylated derivatives (14).Following the pioneering work of Gosio (23) and Challenger et al. (6, 7), the mechanism for the biomethylation of arsenic has been studied extensively with various fungi (10, 15), bacteria (3), archaea (3, 30), and mammals (1, 38, 41), including humans (13). As proposed by Challenger, the biomethylation of selenium and tellurium follows the same mechanism as arsenic but, in general, studies investigating these processes are rather rare (16,22,38). Mercury biomethylation, on the other hand, has been studied in more detail because of poisonings by methylmercury compounds (4,8,29,33). Although stibine has been implicated as a cause in sudden infant death syndrome (31), up to now, only trimethylantimony was detected as a biovolatilization product of antimony in the headspace of soil samples (24) and of pure cultures of Scopulariopsis brevicaulis (12,27). The biomethylation of inorg...

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“…(CH 3 ) 3 Bi present in exhaled air was determined via low temperature (LT)-GC/ICP-MS as described by Wickenheiser et al (1998): a 3-liter subsample (three subsamples of 3 liters per Tedlar bag) was sucked slowly with a vacuum pump and a gas clock (Trommel-Gaszaehler TG 1/6; Ritter Apparatebau, Bochum, Germany) through a trap held at Ϫ78°C. At the same time, the chromatographic column temperature was maintained at Ϫ196°C.…”

Section: Methodsmentioning

confidence: 99%

Determination of Trimethylbismuth in the Human Body after Ingestion of Colloidal Bismuth Subcitrate

Boertz¹,

Hartmann²,

Sułkowski³

et al. 2008

Drug Metab Dispos

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ABSTRACT:Biological methylation and hydride formation of metals and metalloids are ubiquitous environmental processes that can lead to the formation of chemical species with significantly increased mobility and toxicity. Whereas much is known about the interaction of metal(loid)s with microorganisms in environmental settings, little information has been gathered on respective processes inside the human body as yet. Here, we studied the biotransformation and excretion of bismuth after ingestion of colloidal bismuth subcitrate (215 mg of bismuth) to 20 male human volunteers. Bismuth absorption in the stomach and upper intestine was very low, as evidenced by the small quantity of bismuth eliminated via the renal route. Total bismuth concentrations in blood increased rapidly in the first hour after ingestion. Most of the ingested bismuth was excreted via feces during the study period. Trace levels of the metabolite trimethylbismuth [(CH 3 ) 3 Bi] were detected via low temperaturegas chromatography/inductively coupled plasma-mass spectrometry in blood samples and in exhaled air samples. Concentrations were in the range of up to 2.50 pg/ml (blood) and 0.8 to 458 ng/m 3 (exhaled air), with high interindividual variation being observed. Elimination routes of bismuth were exhaled air (up to 0.03‰), urine (0.03-1.2%), and feces. The site of (CH 3 ) 3 Bi production could not be identified in the present study, but the intestinal microflora seems to be involved in this biotransformation if accompanying ex vivo studies are taken into consideration.It is a well known fact that the toxicity of metal(loid)s is essentially dependent on the chemical form, i.e., on the species of the element in question (Craig, 2003;Dopp et al., 2004; Hirner and Emons, 2004). In particular, alkylation often seems to considerably increase the toxic potential of metal(loid)s. Many studies have shown that in the environment methylated and also, in some cases, hydride species can be formed by different mechanisms and from a variety of metal(loid)s (Craig, 2003). In particular, microorganisms, e.g., bacteria and fungi, have been reported to be involved in this specific kind of conversion (Thayer, 2002).In contrast to the considerable knowledge that has accumulated on the interaction of microorganisms with metal(loid)s in the environment, a paucity of information is currently available on the respective processes inside the human body. This lack of knowledge is particularly striking in view of the fact that certain segments of the digestive tract, namely, the oral cavity and the colon, are colonized by myriads of bacteria. The difficulty of analyzing metal(loid) organic compounds at trace and even ultratrace levels might at least partly account for this information gap.After a pilot study with three volunteers , we performed an ingestion experiment with bismuth, administering this element as a single p.o. dose to 20 male volunteers in the form of a therapeutically used colloidal bismuth subcitrate compound. Bismuth was chosen as the element of interest ...

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Development and application of liquid and gas-chromatographic speciation techniques with element specific (ICP-MS) detection to the study of anaerobic arsenic metabolism

Cited by 31 publications

References 0 publications

A review on completing arsenic biogeochemical cycle: Microbial volatilization of arsines in environment

A review on completing arsenic biogeochemical cycle: Microbial volatilization of arsines in environment

Production of Volatile Derivatives of Metal(loid)s by Microflora Involved in Anaerobic Digestion of Sewage Sludge

Determination of Trimethylbismuth in the Human Body after Ingestion of Colloidal Bismuth Subcitrate

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