Development and Application of CRISPR/Cas System in Rice

Ren, Jun; Hu, Xixun; Wang, Kejian; Chün, Wang

doi:10.1016/j.rsci.2019.01.001

Cited by 12 publications

(8 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genome editing employs site-specific nucleases (SSNs) that are premeditated to bind and create a break in a particular nucleic acid site, generating double-stranded breaks (DSBs) at or close to the target site (Pickar-Oliver and Gersbach, 2019). The DNA DSBs are then repaired naturally either through homologous recombination (HR) or errorprone non-homologous end joining (NHEJ) (Miglani, 2017;Wright et al, 2018;Jun et al, 2019). These DSBs repair can be governed to obtain the ideal modifications in a sequence for instance insertions or deletions of large transgene arrays [160].…”

Section: Genome Editingmentioning

confidence: 99%

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Genome Editingmentioning

confidence: 99%

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The double-stranded (ds) DNA is cleaved at particular loci by means of mainly three programmable SSNs, viz., zinc finger nucleases (ZFNs), transcriptional activator-like effector nucleases (TALENs), and CRISPR-Cas9 [161,164,165]. The DNA DSBs then undergo natural repair, via either homologous recombination (HR) or error-prone nonhomologous end joining (NHEJ) [166][167][168]. These DSBs repair can be controlled to achieve the desired sequence modifications such as DNA deletions or insertions of larges arrays of transgenes [160].…”

Section: Use Of Sequence Specific Nucleases For Precise Gene Editing ...mentioning

confidence: 99%

“…If it occurs in the coding region of the gene, it may cause frame shift mutation, resulting in a target gene knockout [170]. On the other hand, the HR mechanism accurately repairs DNA DSBs by integrating a DNA donor containing homologous overhangs at the target site [166,171]. For detailed reviews on DNA DSBs repair mechanisms, we refer you to previous articles [164,167,172,173].…”

Section: Use Of Sequence Specific Nucleases For Precise Gene Editing ...mentioning

confidence: 99%

Advances in Cereal Crop Genomics for Resilience under Climate Change

Zenda

Liu

Dong

et al. 2021

Life

View full text Add to dashboard Cite

Adapting to climate change, providing sufficient human food and nutritional needs, and securing sufficient energy supplies will call for a radical transformation from the current conventional adaptation approaches to more broad-based and transformative alternatives. This entails diversifying the agricultural system and boosting productivity of major cereal crops through development of climate-resilient cultivars that can sustainably maintain higher yields under climate change conditions, expanding our focus to crop wild relatives, and better exploitation of underutilized crop species. This is facilitated by the recent developments in plant genomics, such as advances in genome sequencing, assembly, and annotation, as well as gene editing technologies, which have increased the availability of high-quality reference genomes for various model and non-model plant species. This has necessitated genomics-assisted breeding of crops, including underutilized species, consequently broadening genetic variation of the available germplasm; improving the discovery of novel alleles controlling important agronomic traits; and enhancing creation of new crop cultivars with improved tolerance to biotic and abiotic stresses and superior nutritive quality. Here, therefore, we summarize these recent developments in plant genomics and their application, with particular reference to cereal crops (including underutilized species). Particularly, we discuss genome sequencing approaches, quantitative trait loci (QTL) mapping and genome-wide association (GWAS) studies, directed mutagenesis, plant non-coding RNAs, precise gene editing technologies such as CRISPR-Cas9, and complementation of crop genotyping by crop phenotyping. We then conclude by providing an outlook that, as we step into the future, high-throughput phenotyping, pan-genomics, transposable elements analysis, and machine learning hold much promise for crop improvements related to climate resilience and nutritional superiority.

show abstract

“…Cas12a editing has been widely utilized in many crops (see Table 1 ) including rice ( Endo A. et al, 2016 ; Begemann et al, 2017 ; Hu et al, 2017 ; Tang et al, 2017 , 2018 ; Wang et al, 2017a , b ; Yin et al, 2017 ; Li L. et al, 2018 ; Li et al, 2019a ; Jun et al, 2019 ; Malzahn et al, 2019 ; Banakar et al, 2020 ; Chen et al, 2020 ; Schindele and Puchta, 2020 ), wheat ( Liu et al, 2020 ), maize ( Lee K. et al, 2019 ), soybean ( Kim et al, 2017 ), cotton ( Li B. et al, 2019 ), tomato ( van Vu et al, 2020 ), citrus ( Jia et al, 2019 ), tobacco ( Endo A. et al, 2016 ; Endo and Toki, 2019 ), and the model plant Arabidopsis ( Wolter and Puchta, 2019 ; Schindele and Puchta, 2020 ). At present, three Cas12a genome editing systems AsCas12a, FnCas12a, and LbCas12a have been demonstrated in plants ( Zhong et al, 2018 ) with varied efficiency.…”

Section: Application Of Crispr-cas12a In Agriculturementioning

confidence: 99%

CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement

et al. 2020

View full text Add to dashboard Cite

Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion from today. To meet the demands of growing, geographically and socio-economically diversified nations, we need to diversity and expand agricultural production. This expansion of agricultural productivity will need to occur under increasing biotic, and environmental constraints driven by climate change. Clustered regularly interspaced short palindromic repeats-site directed nucleases (CRISPR-SDN) and similar genome editing technologies will likely be key enablers to meet future agricultural needs. While the application of CRISPR-Cas9 mediated genome editing has led the way, the use of CRISPR-Cas12a is also increasing significantly for genome engineering of plants. The popularity of the CRISPR-Cas12a, the type V (class-II) system, is gaining momentum because of its versatility and simplified features. These include the use of a small guide RNA devoid of trans-activating crispr RNA, targeting of T-rich regions of the genome where Cas9 is not suitable for use, RNA processing capability facilitating simpler multiplexing, and its ability to generate double strand breaks (DSB) with staggered ends. Many monocot and dicot species have been successfully edited using this Cas12a system and further research is ongoing to improve its efficiency in plants, including improving the temperature stability of the Cas12a enzyme, identifying new variants of Cas12a or synthetically producing Cas12a with flexible PAM sequences. In this review we provide a comparative survey of CRISPR-Cas12a and Cas9, and provide a perspective on applications of CRISPR-Cas12 in agriculture.

show abstract

Development and Application of CRISPR/Cas System in Rice

Cited by 12 publications

References 60 publications

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

Advances in Cereal Crop Genomics for Resilience under Climate Change

CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement

Contact Info

Product

Resources

About