Development and Application of CRISPR/Cas System in Rice

Ren, Jun; Hu, Xixun; Wang, Kejian; Chün, Wang

doi:10.1016/j.rsci.2019.01.001

Cited by 12 publications

(3 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genome editing employs site-specific nucleases (SSNs) that are premeditated to bind and create a break in a particular nucleic acid site, generating double-stranded breaks (DSBs) at or close to the target site (Pickar-Oliver and Gersbach, 2019). The DNA DSBs are then repaired naturally either through homologous recombination (HR) or errorprone non-homologous end joining (NHEJ) (Miglani, 2017;Wright et al, 2018;Jun et al, 2019). These DSBs repair can be governed to obtain the ideal modifications in a sequence for instance insertions or deletions of large transgene arrays [160].…”

Section: Genome Editingmentioning

confidence: 99%

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Genome Editingmentioning

confidence: 99%

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Cas12a editing has been widely utilized in many crops (see Table 1 ) including rice ( Endo A. et al, 2016 ; Begemann et al, 2017 ; Hu et al, 2017 ; Tang et al, 2017 , 2018 ; Wang et al, 2017a , b ; Yin et al, 2017 ; Li L. et al, 2018 ; Li et al, 2019a ; Jun et al, 2019 ; Malzahn et al, 2019 ; Banakar et al, 2020 ; Chen et al, 2020 ; Schindele and Puchta, 2020 ), wheat ( Liu et al, 2020 ), maize ( Lee K. et al, 2019 ), soybean ( Kim et al, 2017 ), cotton ( Li B. et al, 2019 ), tomato ( van Vu et al, 2020 ), citrus ( Jia et al, 2019 ), tobacco ( Endo A. et al, 2016 ; Endo and Toki, 2019 ), and the model plant Arabidopsis ( Wolter and Puchta, 2019 ; Schindele and Puchta, 2020 ). At present, three Cas12a genome editing systems AsCas12a, FnCas12a, and LbCas12a have been demonstrated in plants ( Zhong et al, 2018 ) with varied efficiency.…”

Section: Application Of Crispr-cas12a In Agriculturementioning

confidence: 99%

CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement

et al. 2020

View full text Add to dashboard Cite

Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion from today. To meet the demands of growing, geographically and socio-economically diversified nations, we need to diversity and expand agricultural production. This expansion of agricultural productivity will need to occur under increasing biotic, and environmental constraints driven by climate change. Clustered regularly interspaced short palindromic repeats-site directed nucleases (CRISPR-SDN) and similar genome editing technologies will likely be key enablers to meet future agricultural needs. While the application of CRISPR-Cas9 mediated genome editing has led the way, the use of CRISPR-Cas12a is also increasing significantly for genome engineering of plants. The popularity of the CRISPR-Cas12a, the type V (class-II) system, is gaining momentum because of its versatility and simplified features. These include the use of a small guide RNA devoid of trans-activating crispr RNA, targeting of T-rich regions of the genome where Cas9 is not suitable for use, RNA processing capability facilitating simpler multiplexing, and its ability to generate double strand breaks (DSB) with staggered ends. Many monocot and dicot species have been successfully edited using this Cas12a system and further research is ongoing to improve its efficiency in plants, including improving the temperature stability of the Cas12a enzyme, identifying new variants of Cas12a or synthetically producing Cas12a with flexible PAM sequences. In this review we provide a comparative survey of CRISPR-Cas12a and Cas9, and provide a perspective on applications of CRISPR-Cas12 in agriculture.

show abstract

“…Several alleles of genes associated with economic traits have been characterized mainly in japonica genotype (Li et al 2018;Yao et al 2018). Many of these genes for tolerance to biotic and abiotic stresses, nutritional quality improvement, flowering, grain quality and yield traits have been edited by using TALENs, CRISPR-Cas9, CRISPR-Cpf1 and base editing methods (reviewed in Mishra et al 2018;Jun et al 2019). CRISPR-Cas9 approach can be used to create these economically important mutants in indica varieties, which occupy about 80% of rice cultivated area in the world.…”

Section: Introductionmentioning

confidence: 99%

CRISPR-Cas9 mediated genome editing of drought and salt tolerance (OsDST) gene in indica mega rice cultivar MTU1010

Kumar

Verma

Yadav

et al. 2020

Physiol Mol Biol Plants

183

View full text Add to dashboard Cite

Development of abiotic stress tolerant rice cultivars is necessary for sustainable rice production under the scenario of global climate change, dwindling fresh water resources and increase in salt affected areas. Several genes from rice have been functionally validated by using EMS mutants and transgenics. Often, many of these desirable alleles are not available indica rice which is mainly cultivated, and where available, introgression of these alleles into elite cultivars is a time and labour intensive process, in addition to the potential introgression of non-desirable genes due to linkage. CRISPR-Cas technology helps development of elite cultivars with desirable alleles by precision gene editing. Hence, this study was carried out to create mutant alleles of drought and salt tolerance (DST) gene by using CRISPR-Cas9 gene editing in indica rice cv. MTU1010. We used two different gRNAs to target regions of DST protein that might be involved in protein-protein interaction and successfully generated different mutant alleles of DST gene. We selected homozygous dst mutant with 366 bp deletion between the two gRNAs for phenotypic analysis. This 366 bp deletion led to the deletion of amino acid residues from 184 to 305 in frame, and hence the mutant was named as dst D184-305 . The dst D184-305 mutation induced by CRISPR-Cas9 method in DST gene in indica rice cv. MTU1010 phenocopied EMS-induced dst (N69D) mutation reported earlier in japonica cultivar. The dst D184-305 mutant produced leaves with broader width and reduced stomatal density, and thus enhanced leaf water retention under dehydration stress. Our study showed that the reduction in stomatal density in loss of function mutants of dst is, at least, in part due to downregulation of stomatal developmental genes SPCH1, MUTE and ICE1. The Cas9-free dst D184-305 mutant exhibited moderate level tolerance to osmotic stress and high level of salt stress in seedling stage. Thus, dst mutant alleles generated in this study will be useful for improving drought and salt tolerance and grain yield in indica rice cultivars.

show abstract

Development and Application of CRISPR/Cas System in Rice

Cited by 12 publications

References 60 publications

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement

CRISPR-Cas9 mediated genome editing of drought and salt tolerance (OsDST) gene in indica mega rice cultivar MTU1010

Contact Info

Product

Resources

About