Development and application of an oligonucleotide microarray and real-time quantitative PCR for detection of wastewater bacterial pathogens

Lee, Dae-Young; Lauder, Heather; Cruwys, Heather; Falletta, Patricia; Beaudette, Lee A.

doi:10.1016/j.scitotenv.2008.03.004

Cited by 82 publications

(52 citation statements)

References 30 publications

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“…The qPCR primers and fluorescent TaqMan R probes (Table 1) were designed using the computer software Primer Express R v 2.0 (Applied Biosystems), according to Lee et al (2008).…”

Section: Real-time Pcr Methodsmentioning

confidence: 99%

Disinfection of water and wastewater by UV-A and UV-C irradiation: application of real-time PCR method

Chatzisymeon

Droumpali

Mantzavinos

et al. 2011

Photochem Photobiol Sci

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PapersPerformance of the photo-Fenton process in the degradation of a model azo dye mixture J. Macías-Sánchez et al., Photochem. Photobiol. Sci., 2011, 10 The disinfection efficiency of synthetic and real wastewater by means of UV-A and UV-C irradiation in the presence or absence of TiO 2 was investigated. A reference strain of Escherichia coli suspended in sterile 0.8% (w/v) NaCl aqueous solution was used as a synthetic wastewater, while real wastewater samples were collected from the outlet of the secondary treatment of a municipal wastewater treatment plant. E. coli inactivation was monitored both by the conventional culture technique and by the real-time PCR method. Culture method showed that UV-C irradiation (11 W lamp) achieved total E. coli inactivation of 100% within 3 min of photolytic treatment. On the other hand, UV-A (9 W lamp)/TiO 2 ; [TiO 2 ] = 200 mg L -1 (i.e. best operating conditions) required 60 min to achieve total disinfection of the synthetic wastewater. Real time PCR revealed compatible results, regarding the better efficiency of UV-C. However, it showed different times of bacterial inactivation, probably due to the phenomenon of "viable but not culturable bacteria". Disinfection durability tests in the dark and under natural sunlight irradiation showed that there is cell repair when UV-C irradiation is used for synthetic wastewater disinfection. Regarding real wastewater it was observed that only UV-C irradiation was capable of totally inactivating E. coli population in short time. Comparing results obtained from both methods, real time PCR proved to be more reliable and accurate, concerning the bacterial detection and enumeration in aquatic samples after the application of UV irradiation.

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“…The qPCR primers and fluorescent TaqMan R probes (Table 1) were designed using the computer software Primer Express R v 2.0 (Applied Biosystems), according to Lee et al (2008).…”

Section: Real-time Pcr Methodsmentioning

confidence: 99%

Disinfection of water and wastewater by UV-A and UV-C irradiation: application of real-time PCR method

Chatzisymeon

Droumpali

Mantzavinos

et al. 2011

Photochem Photobiol Sci

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“…Human fecal pollution (i.e., sewage) is known to contain pathogens that pose serious health risks (6,8). Identifying sewage impacts can prevent illness, through public warnings and environmental remediation.…”

mentioning

confidence: 99%

Hollow-Fiber Ultrafiltration and PCR Detection of Human-Associated Genetic Markers from Various Types of Surface Water in Florida

Leskinen

Brownell

Lim

et al. 2010

Appl Environ Microbiol

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“…This is an important aspect relating to the monitoring of harmful algae in particular as threshold levels apply to cell densities as well as toxin levels in certain national monitoring programmes . This problem also extends beyond harmful algae monitoring, where other environmental studies have used molecular techniques to identify and estimate abundances of mixed bacterial communities in food, soil and water (Call et al, 2003;Loy et al, 2005;Lee et al, 2008;Kostić et al, 2010 An existing hierarchical group of oligonucleotide probes specific for the haptophytes P.…”

Section: Resultsmentioning

confidence: 99%

“…In that publication a hierarchical set of oligonucleotide probes targeting the 16S ribosomal rRNA was created to analyse the bacterial samples on the DNA-chip. The use of microarrays as quantitative tools has predominately been based on gene expression and mixed bacterial communities in food, soil and water to identify and estimate abundances (Schena et al 1995;Brodie et al 2006;Kong et al 2009;Lee et al 2008;2010;Call et al 2003;Kostić et al 2010). …”

Section: Microarraysmentioning

confidence: 99%

An evaluation of the applicability of microarrays for monitoring toxic algae in Irish coastal waters

McCoy

Touzet

Fleming

et al. 2012

Environ Sci Pollut Res

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Development and application of an oligonucleotide microarray and real-time quantitative PCR for detection of wastewater bacterial pathogens

Cited by 82 publications

References 30 publications

Disinfection of water and wastewater by UV-A and UV-C irradiation: application of real-time PCR method

Disinfection of water and wastewater by UV-A and UV-C irradiation: application of real-time PCR method

Hollow-Fiber Ultrafiltration and PCR Detection of Human-Associated Genetic Markers from Various Types of Surface Water in Florida

An evaluation of the applicability of microarrays for monitoring toxic algae in Irish coastal waters

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