Development and Application of an Optical Biosensor Immunoassay for Aflatoxin M1 in Bovine Milk

Indyk, Harvey E.; Chetikam, Sowmya; Gill, Brendon D.; Wood, Jackie E; Woollard, David C

doi:10.1007/s12161-019-01621-5

Cited by 14 publications

(3 citation statements)

References 23 publications

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“…Even in that case, the LOD achieved was 3 times higher (16.8 pg/mL) compared with that provided by the proposed sensor. Finally, a label-free SPR sensor for AFM1 determination in milk and milk powder was also reported [ 31 ]. The sensor exhibited a 20 time higher LOD than that of the MZI immunosensor developed with an assay duration of 10 min, although defatting and immunoaffinity separation of AFM1 was required prior to analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, the quest for portable analytical devices led to the development of biosensors that can provide high sensitivity and specificity, fast analysis, and quantitative results usually in real-time [ 21 ]. In the last years, several types of biosensors based on electrochemical [ 22 , 23 , 24 , 25 , 26 ] or optical transducers [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ] have been employed for the detection of AFM1. Amongst these, optical biosensors, based either on label or on label-free signal transduction principles, have considerable advantages over the electrochemical ones, as they exhibit high sensitivity, increased miniaturization potential, and less interference from the sample matrix.…”

Section: Introductionmentioning

confidence: 99%

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Mach-Zehnder Interferometric Immunosensor for Detection of Aflatoxin M1 in Milk, Chocolate Milk, and Yogurt

et al. 2023

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Aflatoxin M1 (AFM1) is detected in the milk of animals after ingestion of aflatoxin B1-contaminated food; since 2002, it has been categorized as a group I carcinogen. In this work, a silicon-based optoelectronic immunosensor for the detection of AFM1 in milk, chocolate milk, and yogurt has been developed. The immunosensor consists of ten Mach–Zehnder silicon nitride waveguide interferometers (MZIs) integrated on the same chip with the respective light sources, and an external spectrophotometer for transmission spectra collection. The sensing arm windows of MZIs are bio-functionalized after chip activation with aminosilane by spotting an AFM1 conjugate with bovine serum albumin. For AFM1 detection, a three-step competitive immunoassay is employed, including the primary reaction with a rabbit polyclonal anti-AFM1 antibody, followed by biotinylated donkey polyclonal anti-rabbit IgG antibody and streptavidin. The assay duration was 15 min with limits of detection of 0.005 ng/mL in both full-fat and chocolate milk, and 0.01 ng/mL in yogurt, which are lower than the maximum allowable concentration of 0.05 ng/mL set by the European Union. The assay is accurate (% recovery values 86.7–115) and repeatable (inter- and intra-assay variation coefficients <8%). The excellent analytical performance of the proposed immunosensor paves the way for accurate on-site AFM1 determination in milk.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mach-Zehnder Interferometric Immunosensor for Detection of Aflatoxin M1 in Milk, Chocolate Milk, and Yogurt

et al. 2023

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“…Nevertheless, biosensor setups have successfully been used for a variety of sensing applications in milk. Examples include the investigation of liquid properties and the detection of bacteria, proteins, drugs, hormones, pesticides, and mycotoxins, i.e., the scope of analytical tasks required for milk samples can be covered by biosensors [ 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. Biosensor detectors typically use electrochemical, optical, or acoustic signal transduction.…”

Section: Introductionmentioning

confidence: 99%

Bulk and Surface Acoustic Wave Biosensors for Milk Analysis

Länge

2022

Biosensors

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Milk and dairy products are common foods and, therefore, are subject to regular controls. Such controls cover both the identification and quantification of specific components and the determination of physical parameters. Components include the usual milk ingredients, mainly carbohydrates, proteins, and fat, and any impurities that may be present. The latter range from small molecules, such as drug residues, to large molecules, e.g., protein-based toxins, to pathogenic microorganisms. Physical parameters of interest include viscosity as an indicator of milk gelation. Bulk and surface acoustic wave sensors, such as quartz crystal microbalance (QCM) and surface acoustic wave (SAW) devices, can principally be used for both types of analysis, with the actual application mainly depending on the device coating and the test format. This review summarizes the achievements of acoustic sensor devices used for milk analysis applications, including the determination of physical liquid parameters and the detection of low- and high-molecular-weight analytes and microorganisms. It is shown how the various requirements resulting from the respective analytes and the complex sample matrix are addressed, and to what extent the analytical demands, e.g., with regard to legal limits, are met.

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The research of aptamer biosensor technologies for detection of microorganism

Xiao

et al. 2020

Appl Microbiol Biotechnol

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Development and Application of an Optical Biosensor Immunoassay for Aflatoxin M1 in Bovine Milk

Cited by 14 publications

References 23 publications

Mach-Zehnder Interferometric Immunosensor for Detection of Aflatoxin M1 in Milk, Chocolate Milk, and Yogurt

Mach-Zehnder Interferometric Immunosensor for Detection of Aflatoxin M1 in Milk, Chocolate Milk, and Yogurt

Bulk and Surface Acoustic Wave Biosensors for Milk Analysis

The research of aptamer biosensor technologies for detection of microorganism

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