Development and application of aerobic, chemically defined media for Dysgonomonas

Bridges, Charles M.; Gage, Daniel J.

doi:10.1016/j.anaerobe.2020.102302

Cited by 26 publications

(15 citation statements)

References 84 publications

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“…It is interesting that the sago pith is dominated by Genus Dysgonomonas. This was different from previous studies which reported that species of this genus were found from clinical sources, associated with insects [28,29], sugarcane bagasse [30], food waste and its fermentation [31][32][33], but has never been reported to be found in sago pith.…”

Section: Bacterial Community Structure and Its Potential Usescontrasting

confidence: 99%

Bacterial Community Structure in Sago Pith and Sago Waste Water and Its Potential Uses as Organic Acids Producer

Tenriawaru

Suharjono

Ardyati

et al. 2022

J.Trop.Life.Science

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Sago is one of commodities in South Sulawesi. The extraction process of sago flour produces waste water that still contains organic matter and smells sour. So, it has the potential as a pollutant. The sour odor produced allows the presence of acid-producing bacteria that can be used for waste treatment. The objective of this research is to explore the bacterial community structure in sago pith and sago waste water through high-throughput sequencing technology and its potential uses as organic acids producer. Samples were obtained from a traditional sago factory in Palopo City, South Sulawesi, Indonesia. The acidity degree, total dissolve solid, and temperature were measured in the sago pool at the sampling area, while the nutrient contents were analyzed by titration method in Testing Laboratory of Food Quality and Food Safety, Brawijaya University. Bacterial cells in the sago waste water were acquired through a multilevel filtering process on filter paper with pore nitrocellulose membrane sizes of 0.45 and 0.20 µm. Total DNA trapped in the nitrocellulose membrane with pore size of 0.20 µm was isolated using FastDNA Spin Kit (MPBIO) and the V3-V4 regions of 16S rDNA (341f-806r) were amplified. Amplicons were analyzed by Miseq of Illumina and further analyzed by Muscle v.3.8.31, QIIME v.1.7.0, and R v.2.15.3. The result shows that nutrient content in sago pith is higher than in sago waste water. Both samples are dominated by Phylum Proteobacteria and share 189 common bacterial species. The dominant bacteria that can produce organic acid in sago pith are Dysgonomonas sp., Propionispira sp., and Lactobacillus pentosus. While, Lactobacillus mali and Gluconobacter frateurii are the dominant organic acid-producer bacteria in sago waste water. Keywords: Bacterial community structure, Next generating sequencing, Sago pith, Sago wastewater

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Section: Bacterial Community Structure and Its Potential Usescontrasting

confidence: 99%

Bacterial Community Structure in Sago Pith and Sago Waste Water and Its Potential Uses as Organic Acids Producer

Tenriawaru

Suharjono

Ardyati

et al. 2022

J.Trop.Life.Science

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“…Comprehensive analysis of LEfSe and Mann-Whitney U tests showed numerous bacteria with high relative abundance in captive lizards, such as those of the order Pseudomonadales in the oral cavity of egg-fed lizards and the families Dysgonomonadaceae and Comamonadaceae in the gut of egg- and frog-fed lizards ( Figures 4 , 5 ). The functions of most of these bacteria are poorly known, but those of the family Dysgonomonadaceae and the genera Fusobacterium ( Brennan and Garrett, 2019 ) and Mycoplasma ( Baseman and Tully, 1997 ) have been proved to be associated with human diseases ( Bridges and Gage, 2021 ). Bacteria of the genus Rothia are commonly found in dental plaque in the oral cavity ( Welch et al, 2016 ) and are associated with heart disease ( Pechal et al, 2018 ) and infectious endocarditis in humans ( Boudewijns et al, 2003 ).…”

Section: Discussionmentioning

confidence: 99%

Dietary Correlates of Oral and Gut Microbiota in the Water Monitor Lizard, Varanus salvator (Laurenti, 1768)

Chen

Liu

et al. 2022

Front. Microbiol.

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Numerous studies have demonstrated that food shapes the structure and composition of the host’s oral and gut microbiota. The disorder of oral and gut microbiota may trigger various host diseases. Here, we collected oral and gut samples from wild water monitor lizards (Varanus salvator) and their captive conspecifics fed with bullfrogs, eggs, and depilated chicken, aiming to examine dietary correlates of oral and gut microbiota. We used the 16S rRNA gene sequencing technology to analyze the composition of the microbiota. Proteobacteria and Bacteroidota were the dominant phyla in the oral microbiota, and so were in the gut microbiota. The alpha diversity of microbiota was significantly higher in the gut than in the oral cavity, and the alpha diversity of oral microbiota was higher in captive lizards than in wild conspecifics. Comparing the relative abundance of oral and gut bacteria and their gene functions, differences among different animal groups presumably resulted from human contact in artificial breeding environments and complex food processing. Differences in gene function might be related to the absolute number and/or the taxonomic abundance of oral and gut microorganisms in the wild and the water environment. This study provides not only basic information about the oral and gut microbiota of captive and wild water monitor lizards, but also an inference that feeding on frogs and aquatic products and reducing human exposure help water monitor lizards maintain a microbiota similar to that in the wild environment.

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“…It is possible that there are further differences between the two strains at the species level. Dysgonomonas has been previously isolated from the guts of the subterranean termite Reticulitermes speratus and researchers suggest this genus requires heme to grow 35 , 36 . Dysgonomonas has not been well-studied, especially outside of human guts, so it would be inappropriate to draw conclusions on insecticide resistance based on its presence in a resistant cockroach strain.…”

Section: Discussionmentioning

confidence: 99%

Differential microbial responses to antibiotic treatments by insecticide-resistant and susceptible cockroach strains (Blattella germanica L.)

Wolfe

Scharf

2021

Sci Rep

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The German cockroach (Blattella germanica L.) is a major urban pest worldwide and is known for its ability to resist insecticides. Past research has shown that gut bacteria in other insects can metabolize xenobiotics, allowing the host to develop resistance. The research presented here determined differences in gut microbial composition between insecticide-resistant and susceptible German cockroaches and compared microbiome changes with antibiotic treatment. Cockroaches received either control diet or diet plus kanamycin (KAN) to quantify shifts in microbial composition. Additionally, both resistant and susceptible strains were challenged with diets containing the insecticides abamectin and fipronil in the presence and absence of antibiotic. In both strains, KAN treatment reduced feeding, leading to higher doses of abamectin and fipronil being tolerated. However, LC50 resistance ratios between resistant and susceptible strains decreased by half with KAN treatment, suggesting gut bacteria mediate resistance. Next, whole guts were isolated, bacterial DNA extracted, and 16S MiSeq was performed. Unlike most bacterial taxa, Stenotrophomonas increased in abundance in only the kanamycin-treated resistant strain and was the most indicative genus in classifying between control and kanamycin-treated cockroach guts. These findings provide unique insights into how the gut microbiome responds to stress and disturbance, and important new insights into microbiome-mediated insecticide resistance.

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Development and application of aerobic, chemically defined media for Dysgonomonas

Cited by 26 publications

References 84 publications

Bacterial Community Structure in Sago Pith and Sago Waste Water and Its Potential Uses as Organic Acids Producer

Bacterial Community Structure in Sago Pith and Sago Waste Water and Its Potential Uses as Organic Acids Producer

Dietary Correlates of Oral and Gut Microbiota in the Water Monitor Lizard, Varanus salvator (Laurenti, 1768)

Differential microbial responses to antibiotic treatments by insecticide-resistant and susceptible cockroach strains (Blattella germanica L.)

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