Development and application of a quantitative real-time PCR method for detection of Decapod iridescent virus 1
Fu-Rong Zhao,
Yang Liu,
Qin Zheng
et al.
Abstract:As a newly discovered virus, Decapoda iridovirus 1 (DIV1) can cause a mortality rate of up to 100% in crustaceans, leading to huge economic losses. At present, there is no effective prevention and control measures for this disease. In the present study, the specific primers targeting highly conserved regions of MCP gene were designed, and then a quantitative real-time PCR method was established. The results indicate that DIV1 quantitative real-time PCR established has good specificity and does not cross react … Show more
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