Development and Application of a New Scheme for Typing
            <i>Campylobacter jejuni</i>
            and
            <i>Campylobacter coli</i>
            by PCR-Based Restriction Fragment Length Polymorphism Analysis

Feng, Luzhao; Chen, Yuen Yuen; Wassenaar, Trudy M.; Woods, Walter H.; Coloe, Peter J.; Fry, Benjamin N.

doi:10.1128/jcm.40.5.1791-1797.2002

Cited by 19 publications

(26 citation statements)

References 36 publications

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“…In a previous study (20), a correlation between Penner serotypes and PCR-RFLP types and variations in the RFLP types of strains within a single Penner serotype were also observed. In addition to confirming these findings, our study demonstrated that the PCR-RFLP method is applicable to the typing and classification of NT strains.…”

Section: Discussionmentioning

confidence: 99%

“…Ten NCTC reference strains were also included. The aim of the study was to see if there was a correlation between Penner HS serotypes and RFLP types, as previously described by Shi et al (20). We were especially interested in finding out if this method would be applicable to the serotyping of NT strains by genotypic means.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the antisera are expensive. We used a PCR-restriction fragment length polymorphism (RFLP) method based on the LOS biosynthesis genes (20) to study the correlation between Penner serotypes and PCR-RFLP types and to see if this method was applicable to typing of the strains which are not typeable by serotyping.…”

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confidence: 99%

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Comparative Typing of Campylobacter jejuni by Heat-Stable Serotyping and PCR-Based Restriction Fragment Length Polymorphism Analysis

Nakari¹,

Laaksonen²,

Korkeila³

et al. 2005

J Clin Microbiol

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Campylobacter jejuni has become the most common bacterial cause of human gastroenteritis worldwide. Rapid, discriminatory typing methods are required to identify potential clusters of infections. The major disadvantage of the well-evaluated and widely used Penner heat-stable serotyping method is the high level of nontypeability. The correlation of the types determined by the Penner heat-stable serotyping method and PCR-based restriction fragment length polymorphism (RFLP) analysis of the lipooligosaccharide (LOS) biosynthesis genes of C. jejuni was studied with 149 C. jejuni strains. Of these strains, 79 were patient strains belonging to 25 Penner serotypes, 60 were nontypeable patient strains, and 10 were reference strains. A 9.6-kb DNA fragment of the LOS gene cluster was amplified and digested with the restriction enzymes HhaI and DdeI. Altogether, 39 different RFLP types (including 30 HhaI profiles and 32 DdeI profiles) were identified. Type Hh1Dd1 was the most common type, with 36% of the strains and strains of 12 serotypes being of this type. A high level of discrimination was obtained, and a correlation between the Penner serotypes and the PCR-RFLP types could be seen. Also, variation in the LOS biosynthesis genes within a single Penner serotype was found. Although the PCR-RFLP method may not be sufficient to compensate for Penner serotyping, it can give valuable information about nontypeable strains and further characterize strains of common serotypes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Comparative Typing of Campylobacter jejuni by Heat-Stable Serotyping and PCR-Based Restriction Fragment Length Polymorphism Analysis

Nakari¹,

Laaksonen²,

Korkeila³

et al. 2005

J Clin Microbiol

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“…The LOS gene (LG) typing scheme was established by PCRrestriction fragment length polymorphism of the wla gene cluster (11). Some of the genes in the same cluster were shown to be involved in protein glycosylation in C. jejuni strain 81-176 (12).…”

Section: Authors' Replymentioning

confidence: 99%

Molecular Typing of Campylobacter jejuni and Campylobacter coli

Lastovica

2003

J Clin Microbiol

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“…Sequencing of specific genes has shown to be highly efficient in the study of genetic variability of C. jejuni, specially gene fla A, which encodes the protein flagellin, the main monomeric subunit of the flagellum (7,9,14,15,21).…”

Section: Introductionmentioning

confidence: 99%

Molecular subtyping of Campylobacter jejuni subsp. jejuni strains isolated from different animal species in the state of São Paulo, Brazil

Scarcelli¹,

Piatti²,

Harakava³

et al. 2005

Braz. J. Microbiol.

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The objective of the present trial was to characterize genetically strains of Campylobacter jejuni subsp. jejuni isolated from humans and several animal sources (bovines, swine, dogs, primates, wild boars and poultry). A total of 828 different animal samples (feces, carcass, aborted fetus and hysterectomized uterus) were analysed by means of routine bacteriological methods, and 36 C. jejuni strains were isolated. Thirty strains of human fecal origin were obtained in clinical analysis laboratories in the city of São Paulo. The 66 C. jejuni strains isolated were submitted to genetic characterization. Primers based on fla A gene were used in a polymerase chain reaction (PCR) and amplified a fragment of the 702 bp. PCR products were evaluated by means of sequencing and genealogic analysis. Genetic variability analysis of 66 strains showed 44 different subtypes of C. jejuni. One subtype was identical to a C. jejuni strain of human origin with the sequence in the GenBank (GENBANK -accession number AF050186). Subtyping analysis of C. jejuni strains based on sequencing of the fla A gene variable region and analysis of sequence alignment by the Maximum Parsimony method showed to be highly discriminatory, providing the best conditions to differentiate strains involved in outbreaks from those sporadically isolated. This is the first study of molecular subtyping analysis of human and animal C. jejuni strains using sequencing technique and genealogic analysis in the state of São Paulo, Brazil.

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Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis

Cited by 19 publications

References 36 publications

Comparative Typing of Campylobacter jejuni by Heat-Stable Serotyping and PCR-Based Restriction Fragment Length Polymorphism Analysis

Comparative Typing of Campylobacter jejuni by Heat-Stable Serotyping and PCR-Based Restriction Fragment Length Polymorphism Analysis

Molecular Typing of Campylobacter jejuni and Campylobacter coli

Molecular subtyping of Campylobacter jejuni subsp. jejuni strains isolated from different animal species in the state of São Paulo, Brazil

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