Development and application of a quantitative lateral flow immunoassay for fumonisins in maize

Anfossi, Laura; Calderara, Marianna; Baggiani, Claudio; Giovannoli, Cristina; Arletti, Enrico; Giraudi, Gianfranco

doi:10.1016/j.aca.2010.09.045

Cited by 84 publications

(80 citation statements)

References 20 publications

(33 reference statements)

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“…Indeed, LFIAs combine the advantages of immunoassays, such as applicability to a wide range of analytes, high specificity, high detectability and little or no need for preanalytical sample preparation, with the simplic-ity and rapidity of the immunochromatographic format [1]. defined positions along the strip determines the formation of coloured bands that are visually detected to provide a qualita-tive result (i.e., presence or absence of the analyte in the sam-ple) or measured with a suitable device (an optical scanner or a smartphone-embedded camera) to provide objective, semi-quantitative information [2][3][4]. The use of alternative labels has recently gained wide acceptance, showing advantages in terms of assay sensitivity, but at the expense of assay simplic-ity and non-instrumental requirements.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence lateral flow immunoassay cartridge with integrated amorphous silicon photosensors array for human serum albumin detection in urine samples

et al. 2016

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A novel and disposable cartridge for chemilumi-nescent (CL)-lateral flow immunoassay (LFIA) with integrat-ed amorphous silicon (a-Si:H) photosensors array was devel-oped and applied to quantitatively detect human serum albu-min (HSA) in urine samples. The presented analytical method is based on an indirect competitive immunoassay using horse-radish peroxidase (HRP) as a tracer, which is detected by adding the luminol/enhancer/hydrogen peroxide CL cocktail. The system comprises an array of a-Si:H photosensors depos-ited on a glass substrate, on which a PDMS cartridge that houses the LFIA strip and the reagents necessary for the CL immunoassay was optically coupled to obtain an integrated analytical device controlled by a portable readout electronics. The method is simple and fast with a detection limit of 2.5 mg L −1 for HSA in urine and a dynamic range up to 850 mg L −1 , which is suitable for measuring physiological levels of HSA in urine samples and their variation in different diseases (micro-and macroalbuminuria). The use of CL de-tection allowed accurate and objective analyte quantification in a dynamic range that extends from femtomoles to pico-moles. The analytical performances of this integrated device were found to be comparable with those obtained using a charge-coupled device (CCD) as a reference off-chip detector. These results demonstrate that integrating the a-Si:H photosensors array with CL-LFIA technique provides com-pact, sensitive and low-cost systems for CL-based bioassays with a wide range of applications for in-field and point-of-care bioanalyses.

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Section: Introductionmentioning

confidence: 99%

Chemiluminescence lateral flow immunoassay cartridge with integrated amorphous silicon photosensors array for human serum albumin detection in urine samples

et al. 2016

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“…Spherical gold nanoparticle of putative diameter comprised between 20 and 40 nm (s-GNPs) were synthesized using the citrate reduction method [32], while multi-branched desert rose-like gold nanoparticles (DR-GNPs) were prepared using a seeding growth approach adapted from that previously reported by Ji et al [11]. TEM analysis allowed to visualize GNP morphology: s-GNPs showed the expected spherical shape (Fig.…”

Section: Gnps Characterizationmentioning

confidence: 99%

“…The antibodies were labeled with s-GNPs as previously described [32] through the adsorption of proteins onto GNPs surface. The s-GNPs were adjusted to the desired pH with carbonate buffer and the amount of Ab defined from the titration was added to 1 mL of s-GNPs.…”

Section: Preparation Of S-gnp-labeled Antibodiesmentioning

confidence: 99%

Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins

et al. 2017

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Graphical abstractBlue desert rose-like gold nanoparticles (DR-GNPs) were synthesized, characterized and applied as label for the ImmunoChromatographic Strip Test (ICST) technique, in which red spherical GNPs (sGNPs) are usually employed. The combined use of the blue DR-GNP and red s-GNPs allowed developing of an intuitive multicolor ICST for the simultaneous detection of Aflatoxin B1 and AbstractDesert rose-like gold nanoparticles (DR-GNPs) that exhibited a blue color due to the plasmon resonance band at 620 nm were prepared. The blue DR-GNPs were obtained through a seeding growth approach and characterized by UV-vis spectroscopy, transmission electron microscopy and dynamic light scattering. The DR-GNPs had a hydrodynamic diameter of ~72 nm and were used as labels for the antibody in an immunochromatographic strip test (ICST). Although the particular shape of DR-GNPs and their higher surface area with respect to spherical GNPs, they demonstrated to be effective as antibody labels. A multicolor ICST for aflatoxin B1 and fumonisins was developed that employs both blue DR-and red spherical GNPs. The multicolor ICST allows for simultaneous rapid determination of the two mycotoxins in maize flour, with visual cut-off levels at 2 µg kg -1 for aflatoxin B1 and 1000 µg kg -1 for fumonisins.

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“…Calibration curves were carried out by plotting the ratio between the intensity of the Test and the Control lines (T/C) for OTA standards (0-1-2-4-8-20 μg/l) versus the log of OTA concentration [29]. The calibration curve was determined by a nonlinear regression analysis of the data using the four-parameter logistic equation.…”

Section: Lateral Flow Immunoassaymentioning

confidence: 99%

Increased sensitivity of lateral flow immunoassay for ochratoxin A through silver enhancement

et al. 2013

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Silver nucleation on gold has been exploited for signal amplification and has found application in several qualitative and quantitative bio-sensing techniques, thanks to the simplicity of the method and the high sensitivity achieved. Very recently, this technique has been tentatively applied to improve performance of gold-based immunoassays. In this work, the exploitation of the signal amplification due to silver deposition on gold nanoparticles has been first applied to a competitive lateral flow immunoassay (LFIA). The signal enhancement due to silver allowed us to strongly reduce the amount of the competitor and of specific antibodies employed to build a LF device for measuring ochratoxin A (OTA), thus determining the attainment of the high sensitive assessment of OTA contamination, with a sensitive gain of more than 10-folds compared to the gold-based LFIA that used the same immunoreagents and to all previously reported LFIA for measuring OTA. In addition, a less sensitive "quantitative"-LFIA could be established, by suitably tuning competitor and antibody amounts, which was characterized by reproducible and accurate OTA determinations (RSD% 6-12%, recovery% 82-117%). The quantitative system allowed a reliable OTA quantification in wines and grape musts at the μg/l level requested by the European legislation, as demonstrated by agreeing results obtained through the "quantitative" silver enhanced-LFIA and a reference HPLC-FLD on 30 samples.

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Development and application of a quantitative lateral flow immunoassay for fumonisins in maize

Cited by 84 publications

References 20 publications

Chemiluminescence lateral flow immunoassay cartridge with integrated amorphous silicon photosensors array for human serum albumin detection in urine samples

Chemiluminescence lateral flow immunoassay cartridge with integrated amorphous silicon photosensors array for human serum albumin detection in urine samples

Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins

Increased sensitivity of lateral flow immunoassay for ochratoxin A through silver enhancement

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