Development and application of a capillary electrophoresis based method for the simultaneous screening of six antibiotics in spiked milk samples

Santos, Sérgio M.; Henriques, Marília; Duarte, Armando C.; Esteves, Valdemar I.

doi:10.1016/j.talanta.2006.05.049

Cited by 107 publications

(62 citation statements)

References 12 publications

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“…Strong acidic deproteinizong agents are not recommended because of the degradation of PENs. However, good recoveries have been described when trichloroacetic acid was used for the precipitation of milk samples containing PENs concentrations higher than the MRLs [14].…”

Section: Sample Preparationmentioning

confidence: 99%

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Importance of Treatment Process on the Analysis of Penicillins in Milk Samples by Capillary Electrophoresis

Rodríguez¹,

Martínez²,

Ibarra³

et al. 2015

Field Effect Electroosmosis - A Novel Phenomenon in Electrokinetics and Its Applications in Capillary Electrophoresis

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Section: Sample Preparationmentioning

confidence: 99%

“…Where L t is the total capillary length (0.57 m), Ld is the capillary length from the injection inlet to the detector (0.32 m), V is the applied voltage (16 kV), and t ion and t eof are the migration times (s) of the analyte and the electroosmotic flow, respectively [14].…”

Section: Optimization Of Background Electrolyte Compositionmentioning

confidence: 99%

Importance of Treatment Process on the Analysis of Penicillins in Milk Samples by Capillary Electrophoresis

Rodríguez¹,

Martínez²,

Ibarra³

et al. 2015

Field Effect Electroosmosis - A Novel Phenomenon in Electrokinetics and Its Applications in Capillary Electrophoresis

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“…These include methods based on spectrophotometry, [6][7][8][9][10][11][12] chemiluminescence, 3,13 fluorometry, 14,15 polarography, 16 and mass spectrometry. 17,18 Electrophoresis capilar, 5,19 and HPLC 20,21 are included when separation techniques are used.…”

Section: Introductionmentioning

confidence: 99%

A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction

Freitas¹,

Silva²,

Araújo³

et al. 2011

J. Braz. Chem. Soc.

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Neste trabalho foi proposto um método de análise em fluxo por multicomutação para a determinação de amoxicilina em formulações farmacêuticas. O método proposto é baseado na reação de diazotização da o-nitroanilina com amoxicilina em meio alcalino, produzindo um composto amarelo que foi monitorado em 435 nm. As características do método foram: faixa linear de 25 até 400 mg L -1 de amoxicilina, limite de detecção de 5,1 mg L -1 , frequência analítica de 50 determinações por hora e desvio padrão relativo inferior a 3,9% (n = 10) para uma amostra típica de 100 mg L -1 amoxicilina. Para verificar a exatidão do método, as amostras foram analisadas empregando um método independente e aplicando-se o teste-t pareado não houve diferença significativa no nível de confiança de 95%.A multicommuted flow analysis method for the spectrophotometric determination of amoxicillin in pharmaceutical formulations is reported. The proposed method is based on the reaction of diazotised o-nitroaniline with amoxicillin in an alkaline medium, producing a yellow compound that was monitored at 435 nm. The main features of the method were: a linear relationship with amoxicillin concentration ranging from 25 to 400 mg L -1 ; a detection limit of 5.1 mg L -1; an analytical throughput of 50 determinations per hour; and a relative standard deviation lower than 3.9% for a typical amoxicillin sample with concentration of 100 mg L -1 (n = 10). To verify the accuracy of the method, samples were also analyzed employing an independent method, and applying the paired t-test to the results no significant difference at the 95% confidence level was observed.

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“…[1][2][3] Devido a isto, o monitoramento dos resíduos de medicamentos veterinários em alimentos tornou-se uma área de preocupação constante dos consumidores, órgãos reguladores e instituições de pesquisa. [1][2][3][4] Na prática veterinária e/ou durante processamento, cloranfenicol (CAP) (Figura 1) pode ser utilizado como antibiótico contra bactérias infecciosas devido ao seu amplo espectro de ação e baixo custo. [5][6][7] Entretanto, na terapêutica humana CAP só é utilizado como fármaco de última escolha quando não existe a disponibilidade de outros fármacos eficazes com menor risco toxicológico.…”

Section: Introductionunclassified

Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE)

Silva¹,

Silva²,

Druzian³

2010

Quím. Nova

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Recebido em 3/2/09; aceito em 21/6/09; publicado na web em 25/11/09 OPTIMIZATION AND INTRALABORATORIAL VALIDATION OF METHOD FOR ANALYSIS OF CHLORAMPHENICOL RESIDUES IN GOAT MILK BY GC/ECD. The presence of chloramphenicol residues in goat milk can cause toxic effects in the population. The present work consists of the optimization and validation of analytical methodology for determination of chloramphenicol residues in goat milk by GC/ECD. The extraction was made with ethyl acetate and the clean-up with SPE-C18. The identification was made by comparison of retention time and GC/MS, and the quantification by external standard. The method was selective, linearity (0.998), precise (5.8-13.4%), exact (69.87-73.71%) and robust. The LOD and LOQ of method were 0.030 and 0.10 μg/kg, respectively. The method was efficiently for analysis of chloramphenicol in goat milk.

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Development and application of a capillary electrophoresis based method for the simultaneous screening of six antibiotics in spiked milk samples

Cited by 107 publications

References 12 publications

Importance of Treatment Process on the Analysis of Penicillins in Milk Samples by Capillary Electrophoresis

Importance of Treatment Process on the Analysis of Penicillins in Milk Samples by Capillary Electrophoresis

A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction

Otimização e validação intralaboratorial de método para análise de resíduos de cloranfenicol em leite caprino por cromatografia gasosa com detecção por captura de elétrons (CG/DCE)

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