Development and application of a sensitive high performance ion-exchange chromatography method for the simultaneous measurement of dopamine, 5-hydroxytryptamine and norepinephrine in microdialysates from the rat brain

Highly sensitive assay for the measurement of serotonin in microdialysates using capillary high-performance liquid chromatography with electrochemical detection

“…Finally, the limits of detection (LOD) and quantification (LOQ) for 5-HT and 5-HIAA were lower than the concentrations measured in the microdialysis samples. In terms of concentrations, our LOD is generally 4-10-fold lower than those previously reported for HPLC-ED [11,15,18]. Moreover, when comparing the mass detected with a classical HPLC-ED method exhibiting the same LOD as ours [12] or even twice smaller [19], our system allows to detect only 84 amol of 5-HT in 1 L, instead of 750-1000 amol in 15-20 L (Table 3), which proves that the miniaturization is not associated with a loss in the electrochemical signal, provided that an appropriate HPLC-ED system is used.…”

“…However, the set-up described by Lorrain's team shows that a manual injection is required and, consequently, a part of the sample is lost. Moreover, we can point out that the HPLC-ED methods used for serotonin are seldom validated for microdialysates [11,12] and in most cases, lack pharmacological validation.…”

Selective Antagonism at Dopamine D3 Receptors Enhances Monoaminergic and Cholinergic Neurotransmission in the Rat Anterior Cingulate Cortex

“…While the three monoamines, NE, DA, and 5-HT, have been detected simultaneously using LC with electrochemical detection (Heidbreder et al, 2001b), ACh was quantified by using a newly developed analytical method based on LC-MS 2 detection (Hows et al, 2002). In comparison with the most commonly used LC methods coupled with electrochemical detection, the LC-MS assay method used in the present study is very specific, minimizing the need to separate ACh from other components present in dialysates.…”

Method for simultaneous measurement of norepinephrine, 3-methoxy-4-hydroxyphenylglycol and 3,4-dihydroxyphenylglycol by liquid chromatography with electrochemical detection: application in rat cerebral cortex and plasma after lithium chloride treatment

“…The conditions have been optimised for using electrochemical detection (ECD). The method can use only a glassy carbon cell and a silver/silver chloride reference electrode [24,25] or a dual-electrode detector with two cells placed in series [26,27]. In this case, NE was oxidized at the upstream electrode and the oxidation product detected at the downstream electrode to increase selectivity.…”