Development and application of a reverse-transcription recombinase-aided amplification assay for subgroup J Avian leukosis virus

Wu, Xiaochan; Yao, Ziqi; Zhao, Qiqi; Chen, S.; Hu, Zhe; Xie, Zili; Chen, L.Y.; Ji, Jun; Chen, F.; Zhang, Xiaohong; Xie, Qingmei

doi:10.1016/j.psj.2022.101743

Cited by 6 publications

(2 citation statements)

References 37 publications

(42 reference statements)

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“…Currently, many molecular assays have been developed such as multiplex PCR, multiplex qPCR, loop-mediated isothermal amplification, and reverse transcription recombinase-aided amplification [ 7 , 8 , 13 , 14 ]. Due to the advantages of simple operation, short duration, and high sensitivity, multiplex qPCR is the most widely used in clinical diagnosis.…”

Section: Discussionmentioning

confidence: 99%

A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses

Dou,

Wang,

et al. 2023

Viruses

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Avian leukosis (AL), caused by avian leukosis virus (ALV), is a contagious tumor disease that results in significant economic losses for the poultry industry. Currently, ALV-A, B, J, and K subgroups are the most common in commercial poultry and cause possible coinfections. Therefore, close monitoring is necessary to avoid greater economic losses. In this study, a novel multiplex quantitative polymerase chain reaction (qPCR) assay was developed to detect ALV-A, ALV-B, ALV-J, and ALV-K with limits of detection of 40, 11, 13.7, and 96 copies/µL, respectively, and no cross-reactivity with other ALV subtypes and avian pathogens. We detected 852 cell cultures inoculated with clinical samples using this method, showing good consistency with conventional PCR and ELISA. The most prevalent ALV strain in Hubei Province, China, was still ALV-J (11.74%). Although single infections with ALV-A, ALV-B, and ALV-K were not found, coinfections with different subgroup strains were identified: 0.7% for ALV-A/J, 0.35% for ALV-B/J, 0.25% for ALV-J/K, and 0.12% for ALV-A/B/K and ALV-A/B/J. Therefore, our novel multiplex qPCR may be a useful tool for molecular epidemiology, clinical detection of ALV, and ALV eradication programs.

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Section: Discussionmentioning

confidence: 99%

A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses

Dou,

Wang,

et al. 2023

Viruses

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“…Avian leukemia virus (ALV) is a carcinogenic retrovirus that can cause tumorous diseases such as lymphoid leukemia and myeloid leukemia in poultry. 1,2 It is one of the pathogens causing serious economic losses to the poultry industry worldwide. 3 Under natural conditions, ALV only infects chickens, with vertical transmission as the main form of transmission, which can lead to a decrease in the immune prevention and controllability of diseased chickens, leading to further multiple infections.…”

Section: Introductionmentioning

confidence: 99%

Establishment and application of a gold nanoparticle-based immunochromatographic test strip for the detection of avian leukosis virus P27 antigen in egg white samples

Wei,

Kuang,

et al. 2024

Analyst

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Rapid Detection of Strawberry Mild Yellow Edge Virus with a Lateral Flow Strip Reverse Transcription Recombinase Polymerase Amplification Assay

Zou

Dong²,

Ni³

et al. 2022

Curr Microbiol

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Development and application of a reverse-transcription recombinase-aided amplification assay for subgroup J Avian leukosis virus

Cited by 6 publications

References 37 publications

A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses

A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses

Establishment and application of a gold nanoparticle-based immunochromatographic test strip for the detection of avian leukosis virus P27 antigen in egg white samples

Rapid Detection of Strawberry Mild Yellow Edge Virus with a Lateral Flow Strip Reverse Transcription Recombinase Polymerase Amplification Assay

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