Development and antigenic characterization of three recombinant proteins with potential for Glässer's disease prevention

Li, Miao; Li, Chunling; Song, Sooyeon; Kang, Huahua; Yang, Dan; Li, Guoqing

doi:10.1016/j.vaccine.2016.03.014

Cited by 11 publications

(19 citation statements)

References 32 publications

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“…Therefore, results of this study contribute to the understanding of the immunogenicity of rOMP10 and rOMP19. These OMPs have been generally studied to develop vaccines against diseases caused by various species, including Anaplasma marginale (29), S. enterica (30), Haemophilus parasuis (31), and E. coli (32). Pathogenic infections against host cells and the consequent cytokine production have been investigated thoroughly in the field of immunology and vaccine development (33)(34)(35)(36).…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Immune Responses to Outer Membrane Antigens OMP10, OMP19, and OMP28 of <i>Brucella abortus</i>

Park

Jung

et al. 2018

Jpn J Infect Dis

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SUMMARY:Brucella infection is accompanied by cytokine production, which serves as an important factor to evaluate the innate and adaptive immune responses. Several researchers have been investigating the mechanisms involved in Brucella infection in the host. Here, we conducted an analytical study to define pathogenic pathways and immune mechanisms involved in Brucella infection by investigating the antigenic efficacy of recombinant outer membrane protein 10 (rOMP10), outer membrane protein 19 (rOMP19), and outer membrane protein 28 (rOMP28) in vitro and in vivo upon stimulation/immunization. Cytokine production was analyzed by nitric oxide (NO) assay and enzyme-linked immunosorbent assay (ELISA) after stimulation of RAW 264.7 cells and naive splenocytes with the recombinant proteins. Our results show that levels of NO, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 increased in RAW 264.7 cells in a time-dependent manner following recombinant protein stimulation. In contrast, levels of interferon (IFN)-γ and IL-2 increased in naive splenocytes after stimulation with proteins. ELISA and ELISpot assays were performed after immunization of mice with recombinant proteins. rOMP28 greatly increased IFN-γ, IL-2, and TNF-α levels than IL-4 and IL-6 levels in vitro. Of the recombinant proteins, rOMP19 elicited a mixed Th1/Th2 immune response by increasing the number of IgG-secreting cells in vivo.

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Immune Responses to Outer Membrane Antigens OMP10, OMP19, and OMP28 of <i>Brucella abortus</i>

Park

Jung

et al. 2018

Jpn J Infect Dis

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“…The H . parasuis serovar 5 H46 was isolated from a pig farm in Guangdong Province, China [18]. For challenge test, H46 was cultured on TSA agar for 16 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Western blotting analysis was carried out using our previous study [17–18]. Convalescent swine sera (1:500 diluted with 5% skim milk in PBST) were added and incubated for 1 h at room temperature (RT) as primary antibody, and goat anti-porcine IgG (H + L)-HRP antibody (1:5,000) (Sigma, USA) was added and incubated at RT for 1 h as secondary antibody.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of immunogenicity and protective efficacy of recombinant outer membrane proteins of Haemophilus parasuis serovar 5 in a murine model

Cai

Song

et al. 2017

PLoS ONE

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Glässer’s disease is an economically important infectious disease of pigs caused by Haemophilus parasuis. Few vaccines are currently available that could provide effective cross-protection against various serovars of H. parasuis. In this study, five OMPs (OppA, TolC, HxuC, LppC, and HAPS_0926) identified by bioinformatic approaches, were cloned and expressed as recombinant proteins. Antigenicity of the purified proteins was verified through Western blotting, and primary screening for protective potential was evaluated in vivo. Recombinant TolC (rTolC), rLppC, and rHAPS_0926 proteins showing marked protection of mice against H. parasuis infection, and were further evaluated individually or in combination. Mice treated with these three OMPs produced humoral and host cell-mediated responses, with a significant rise in antigen-specific IgG titer and lymphoproliferative response in contrast with the mock-immunized group. Significant increases were noted in CD4+, CD8+ T cells, and three cytokines (IL-2, IL-4, and IFN-γ) in vaccinated animals. The antisera against candidate antigens could efficiently impede bacterial survival in whole blood bactericidal assay against H. parasuis infection. The multi-protein vaccine induced more pronounced immune responses and offered better protection than individual vaccines. Our findings indicate that these three OMPs are promising antigens for the development of multi-component subunit vaccines against Glässer's disease.

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Protective efficacy of cationic-PLGA microspheres loaded with DNA vaccine encoding the sip gene of Streptococcus agalactiae in tilapia

Hao

Liang

et al. 2017

Fish & Shellfish Immunology

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Development and antigenic characterization of three recombinant proteins with potential for Glässer's disease prevention

Cited by 11 publications

References 32 publications

Comparative Analysis of Immune Responses to Outer Membrane Antigens OMP10, OMP19, and OMP28 of <i>Brucella abortus</i>

Comparative Analysis of Immune Responses to Outer Membrane Antigens OMP10, OMP19, and OMP28 of <i>Brucella abortus</i>

Evaluation of immunogenicity and protective efficacy of recombinant outer membrane proteins of Haemophilus parasuis serovar 5 in a murine model

Protective efficacy of cationic-PLGA microspheres loaded with DNA vaccine encoding the sip gene of Streptococcus agalactiae in tilapia

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