2012
DOI: 10.1080/00032719.2012.677787
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Development and Analytical Validation of a BT-474 Anti-Proliferation Assay Targeting HER2

Abstract: Human Epidermal Growth Factor Receptor 2 (HER2) is an ideal target for anti-tumor drug development especially for breast cancer. Based on this target, either for new drug, bio-better, bio-similar screening at discovery level, or for drug substance and drug product release at QC or industry level, a reliable and robust biological potency assay is urgently needed. In this study, a BT-474 cell based anti-proliferation biological potency assay targeting HER2 was developed after an optimization of some key aspects,… Show more

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