Developing Transgenic Agronomic Traits for Crops: Targets, Methods, and Challenges

Davies, John P.; Christensen, Cory A.

doi:10.1007/978-1-4939-8778-8_22

Cited by 5 publications

(3 citation statements)

References 104 publications

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“…First, our method of detecting off-target effects is much more sensitive than other reports. Second, during transgenic crop production, the transgene is inserted into the genome of a transformation variety, and then introgressed (repeatedly back-crossed) into the ever-changing elite germplasm before deployment for agriculture 50 . This introgression process segregates away the rest of the genome and places the insertion event into a new genetic background, effectively removing any free-transposition events that are not tightly linked.…”

Section: Discussionmentioning

confidence: 99%

Transposase-assisted target-site integration for efficient plant genome engineering

Liu,

Panda,

Edwards

et al. 2024

Nature

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The current technologies to place new DNA into specific locations in plant genomes are low frequency and error-prone, and this inefficiency hampers genome-editing approaches to develop improved crops1,2. Often considered to be genome ‘parasites’, transposable elements (TEs) evolved to insert their DNA seamlessly into genomes3–5. Eukaryotic TEs select their site of insertion based on preferences for chromatin contexts, which differ for each TE type6–9. Here we developed a genome engineering tool that controls the TE insertion site and cargo delivered, taking advantage of the natural ability of the TE to precisely excise and insert into the genome. Inspired by CRISPR-associated transposases that target transposition in a programmable manner in bacteria10–12, we fused the rice Pong transposase protein to the Cas9 or Cas12a programmable nucleases. We demonstrated sequence-specific targeted insertion (guided by the CRISPR gRNA) of enhancer elements, an open reading frame and a gene expression cassette into the genome of the model plant Arabidopsis. We then translated this system into soybean—a major global crop in need of targeted insertion technology. We have engineered a TE ‘parasite’ into a usable and accessible toolkit that enables the sequence-specific targeting of custom DNA into plant genomes.

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Section: Discussionmentioning

confidence: 99%

Transposase-assisted target-site integration for efficient plant genome engineering

Liu,

Panda,

Edwards

et al. 2024

Nature

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“…During crop production, the transgene is inserted into a transformation strain, and then introgressed (repeatedly back-crossed into) an elite germplasm before deployment for agriculture. This introgression process segregates away the rest of the genome and places the insertion event into a new genetic background, effectively removing any off-target events that are not tightly linked [66][67][68] .…”

Section: Discussionmentioning

confidence: 99%

CRISPR-targeted transposable element insertion for efficient plant genome engineering

Slotkin

Liu

Panda

et al. 2023

Preprint

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The current technologies to place new DNA into specific locations in plant genomes are low frequency and error-prone, and this inefficiency hampers genome editing approaches to develop improved crops. Often considered genome ‘parasites’, transposable elements (TEs) evolved to insert their DNA seamlessly into genomes. TEs select their site of insertion based on preferences for chromatin contexts, which differ for each TE type. We developed a genome engineering tool that controls the TE insertion site and cargo delivered, taking advantage of the TE’s natural ability to precisely insert into the genome. Inspired by CRISPR-associated transposases (CASTs) that target transposition in a programmable manner in bacteria, we generated a synthetic CAST by fusing the rice Pong TE transposase protein to the Cas9 or CPF1 programmable nucleases. We demonstrated sequence-specific targeted delivery (guided by the CRISPR gRNA) of enhancer elements, an open reading frame and gene expression cassette into the genome of the model plant Arabidopsis. We additionally translated this system into soybean, a major global crop in need of targeted insertion technology. We have engineered a TE ‘parasite’ into a usable and accessible toolkit that enables the sequence-specific targeting of custom DNA into plant genomes.

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“…This crop increased production 5.5-folds from 50,000 ha in 2013 to 275,000 ha in 2014. Cold shock protein B (CSPB) inserted from Bacillus subtilis in MON 87460 expresses to imparted drought tolerance 139,140 . In bacteria, cold shock proteins help in preserving normal cellular functions by stabilizing cellular RNA and enhancing gene expression under abiotic stress 141 .…”

Section: Role Of Rhizobacteria On Antioxidant Defense System For Indumentioning

confidence: 99%

Rhizobacteriome: Promising Candidate for Conferring Drought Tolerance in Crops

Yadav¹,

Raghav²,

Sharma³

et al. 2020

J. Pure Appl. Microbiol.

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Drought is a global water shortage problem which poses challenge to crop productivity. Novel strategies are being tried to find out solution to sustain agriculture under drought conditions. Rhizobacteriome is an exclusive genetic material of bacteria resident to rhizosphere plays critical role to health and yield of plant. The interaction of rhizobacteriome with plant provides basis for protecting plants from various abiotic and biotic stresses. Plant growth promoting rhizobacteria (PGPR) are root-colonizing bacteria which produce array of enzymes and metabolites that assist plants to withstand harsh environmental conditions. Various formulations of rhizobacteria are being applied to enhance the tolerance or endurance to drought in crops which in turn increase crop productivity. This could be a one of the promising methods with wide potentiality to improve the growth and yield of crops under limited water resources and changing climatic conditions to ensure food security of the globe. In this review, we summarize (1) existing knowledge and understanding about the rhizobacteria, (2) their role in imparting tolerance to crops in drought conditions and (3) discuss future line of work in this frontier research area.

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Developing Transgenic Agronomic Traits for Crops: Targets, Methods, and Challenges

Cited by 5 publications

References 104 publications

Transposase-assisted target-site integration for efficient plant genome engineering

Transposase-assisted target-site integration for efficient plant genome engineering

CRISPR-targeted transposable element insertion for efficient plant genome engineering

Rhizobacteriome: Promising Candidate for Conferring Drought Tolerance in Crops

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