Developing Peripheral Blood Gene Expression-Based Diagnostic Tests for Coronary Artery Disease: a Review

Rhees, Brian; Wingrove, James A.

doi:10.1007/s12265-015-9641-5

Cited by 7 publications

(7 citation statements)

References 60 publications

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“…As we described previously [ 6 ], EV have an important role in the transport of circulating miRNAs. Several reports show the potential use of miRNAs in the diagnosis and prognosis of cardiac injury [ 3 , 7 , 8 , 43 – 47 ]. Yet, the temporal release profile of miRNAs upon cardiac injury is largely unexplored.…”

Section: Discussionmentioning

confidence: 99%

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Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury

Deddens

Vrijsen

Colijn

et al. 2016

J. of Cardiovasc. Trans. Res.

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Plasma-circulating microRNAs have been implicated as novel early biomarkers for myocardial infarction (MI) due to their high specificity for cardiac injury. For swift clinical translation of this potential biomarker, it is important to understand their temporal and spatial characteristics upon MI. Therefore, we studied the temporal release, potential source, and transportation of circulating miRNAs in different models of ischemia reperfusion (I/R) injury. We demonstrated that extracellular vesicles are released from the ischemic myocardium upon I/R injury. Moreover, we provided evidence that cardiac and muscle-specific miRNAs are transported by extracellular vesicles and are rapidly detectable in plasma. Since these vesicles are enriched for the released miRNAs and their detection precedes traditional damage markers, they hold great potential as specific early biomarkers for MI.Electronic supplementary materialThe online version of this article (doi:10.1007/s12265-016-9705-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

“…Increasing evidence suggests that circulating microRNAs (miRNA) can be potential biomarker candidates due to their highly specific elevation in blood upon stress, including MI [ 3 , 7 , 8 ]. MiRNAs are short (∼22 nucleotides) non-coding RNAs [ 9 ] that regulate gene expression at a post-transcriptional level [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury

Deddens

Vrijsen

Colijn

et al. 2016

J. of Cardiovasc. Trans. Res.

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“…Finally, it is of note that there were little overlapping genes between our GES12, Corus CAD, and a 9-gene signature identified by Muse et al [12]. This phenomenon may be attributed to different strategies for gene signature selection, differences in disease phenotypes, or the complex nature of the pathogenesis of CAD leading to the coexistence of various genesets with certain predictive values [11].…”

Section: Discussionmentioning

confidence: 56%

“…Recently, developing a blood-based gene expression score (GES) has been considered as a promising biomarker strategy for prediction of coronary atherosclerosis [10]. However, despite great efforts in this field, constructing a reproducible gene signature has proved difficult, due to lack of validation in independent cohorts, batch variability in multiple experimental platforms, and low levels of differential expression of single genes [11]. Of note, established GESs, including a 9-gene signature identified by Muse et al [12], a 23-gene classifier (i.e.…”

Section: Introductionmentioning

confidence: 99%

Identification of a blood-based 12-gene signature that predicts the severity of coronary artery stenosis: An integrative approach based on gene network construction, Support Vector Machine algorithm, and multi-cohort validation

et al. 2019

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“…The gene expression profiling in blood leukocytes has been proposed as a suitable approach to identify subjects at risk for CAD, although with several inconsistencies between different studies [24]. In fact, no definite information still exists on the genes truly predisposing to CAD development.…”

Section: Atherosclerosis and Ischaemic Heart Diseasementioning

confidence: 99%

Circulating Leukocytes and Oxidative Stress in Cardiovascular Diseases: A State of the Art

Rubattu

Forte

Raffa

2019

Oxidative Medicine and Cellular Longevity

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Increased oxidative stress from both mitochondrial and cytosolic sources contributes to the development and the progression of cardiovascular diseases (CVDs), and it is a target of therapeutic interventions. The numerous efforts made over the last decades in order to develop tools able to monitor the oxidative stress level in patients affected by CVDs rely on the need to gain information on the disease state. However, this goal has not been satisfactorily accomplished until now. Among others, the isolation of circulating leukocytes to measure their oxidant level offers a valid, noninvasive challenge that has been tested in few pathological contexts, including hypertension, atherosclerosis and its clinical manifestations, and heart failure. Since leukocytes circulate in the blood stream, it is expected that they might reflect quite closely both systemic and cardiovascular oxidative stress and provide useful information on the pathological condition. The results of the studies discussed in the present review article are promising. They highlight the importance of measuring oxidative stress level in circulating mononuclear cells in different CVDs with a consistent correlation between degree of oxidative stress and severity of CVD and of its complications. Importantly, they also point to a double role of leukocytes, both as a marker of disease condition and as a direct contributor to disease progression. Finally, they show that the oxidative stress level of leukocytes reflects the impact of therapeutic interventions. It is likely that the isolation of leukocytes and the measurement of oxidative stress, once adequately developed, may represent an eligible tool for both research and clinical purposes to monitor the role of oxidative stress on the promotion and progression of CVDs, as well as the impact of therapies.

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Developing Peripheral Blood Gene Expression-Based Diagnostic Tests for Coronary Artery Disease: a Review

Cited by 7 publications

References 60 publications

Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury

Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury

Identification of a blood-based 12-gene signature that predicts the severity of coronary artery stenosis: An integrative approach based on gene network construction, Support Vector Machine algorithm, and multi-cohort validation

Circulating Leukocytes and Oxidative Stress in Cardiovascular Diseases: A State of the Art

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