Developing Papaya to Control Papaya Ringspot Virus by Transgenic Resistance, Intergeneric Hybridization, and Tolerance Breeding

Vegas, Ariadne; Prasartsee, Vilai; Drew, R. A. I.; Suzuki, Jon Y.; Tripathi, Savarni

doi:10.1002/9780470650325.ch2

Cited by 15 publications

(21 citation statements)

References 55 publications

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“…Although no resistance has been observed in any genotypes of papaya, some tolerance to PRSV-P has been recorded (Gonsalves et al 2005). Four of the the IPB breeding lines (4108, 4172, 5648 and 5893) that were used in this experiment had shown some tolerance to PRSV-P when grown previously in the Philippines.…”

Section: Discussionmentioning

confidence: 95%

Papaya ringspot virus resistance in Carica papaya via introgression from Vasconcellea quercifolia

et al. 2011

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We report the first successful production of PRSV-P resistant backcross (BC) papaya plants following intergeneric hybridisation between C. papaya and a Vasconcellea species after 50 years of reports on unsuccessful attempts. This follows our previous reports of PRSV-P resistant F 1 hybrids developed by intergeneric hybridisation between C. papaya and V. quercifolia. One PRSV-P resistant BC 1 (BC 1 ) plant was produced after 114,839 seeds were dissected from 940 fruits. The seeds yielded 1,011 embryos and 733 germinated in vitro from which 700 developed into plantlets that were screened in a glasshouse and in the field under high disease pressure and exposure to inoculation by viruliferous aphids. From the PRSV-P resistant backcross 1 (BC 1 ) male plant, 1465 plants [137 BC 2 , 546 SbC 2 (BC2 sib-crosses), 147 BC 3 , 379 SbC 3 and 256 BC 4 ] were grown from seed and inoculated with PRSV-P and virus resistant BC 3 and BC 4 plants were selected from these generations. Presence or absence of virus was confirmed by ELISA serological tests. BC plants generally developed mild symptoms of PRSV-P after periods ranging from 5 to 18 months in the field but many showed the ability to produce new growth free of symptoms. All control plants developed severe symptoms after 3 months in the field. Some BC 3 and BC 4 plants were free from viral infection after 18 months in the field. Subsequently they developed very mild symptoms on their leaves and a few ringspots on their fruit. They continued to grow vigorously and produce fruit for 3 years under high disease pressure provided by the infected controls and other susceptible plants. Good quality marketable fruit were produced on these plants. Application of these results should lead to restoration of the papaya industry in virus-infested regions of the Philippines and worldwide.

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Section: Discussionmentioning

confidence: 95%

Papaya ringspot virus resistance in Carica papaya via introgression from Vasconcellea quercifolia

et al. 2011

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“…In Hawaii, the dominate papaya is the small Hawaiian solo type papaya that weighs an average of 1-2.5 pounds. Although papaya is polygamous, with male, female, and hermaphrodite plants, only the hermaphrodite is grown commercially (Gonsalves et al, 2008) in Hawaii. (Gonsalves et al, 2008).…”

Section: Papaya and Papaya Ringspot Virusmentioning

confidence: 99%

Biolistic and other non-Agrobacterium technologies of plant transformation

Matsumoto

2012

Plant Biotechnology and Agriculture

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“…efficient protocols have resulted from technical improvements in transgene engineering, in transformation of a variety of crops, and in the understanding of the mechanisms of resistance. However, the commercial potential has not yet been realized since only three virusresistant crops have reached the marketplace in the USA (Gonsalves et al, 2006). one approach for achieving virus resistance is based on the phenomenon of cross protection, in which infection by a mild strain of a virus induces resistance to subsequent infection by a more virulent strain.…”

Section: Insect Resistancementioning

confidence: 99%

“…This approach was effective in protecting against the severe strains of PRSv, but produced severe symptoms on certain cultivars, such as 'Sunrise', and thus did not receive widespread use (Pitz et al, 1994). However, one mild strain of PRSv was later used as the source of CP for the PRSv-resistant transgenic papaya currently commercialized in Hawaii (Gonsalves et al, 2006). Another approach uses antisense or cosuppression techniques, which consist in inserting a complementary version of the target gene in an antisense orientation to reduce the amount of the viral protein when the virus infects a plant.…”

Section: Insect Resistancementioning

confidence: 99%

An update on genetically modified crops

Schwember

2008

Cienc. Inv. Agr.

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Genetically modified (GM) crops were introduced in the mid 1990s and two principal transgenic technologies currently dominate the market, herbicide-tolerant (HT) crops and insectresistant crops (Bacillus thuringiensis (Bt) crops). HT crops have simplified weed management practices, reduced crop production costs, and have had positive effects on the environment. However, there are concerns about the potential development of weeds resistant to glyphosate, the main herbicide employed with HT crops. A second major worry associated with the use of HT crops is the potential introgression of genes from GM crops into wild relatives (i.e. gene flow) and its potential impact on natural ecosystems. Bt crops have increased yields and reduced the use of insecticides, providing benefits for human health and the environment. However, the potential development of resistance to the Bt toxin by insect pests and the indirect damage of Bt toxins to non-target species are major concerns related to their use. Different strategies to mitigate and eliminate the problems associated with the use of GM crops are discussed in the paper. The next step in plant biotechnology is the release of nutritionally-enhanced components in seeds that will benefit humans directly.

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Developing Papaya to Control Papaya Ringspot Virus by Transgenic Resistance, Intergeneric Hybridization, and Tolerance Breeding

Cited by 15 publications

References 55 publications

Papaya ringspot virus resistance in Carica papaya via introgression from Vasconcellea quercifolia

Papaya ringspot virus resistance in Carica papaya via introgression from Vasconcellea quercifolia

Biolistic and other non-Agrobacterium technologies of plant transformation

An update on genetically modified crops

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