Developing new parthenocarpic tomato breeding lines carrying iaa9-3 mutation

Tran, Long Thien; Nguyen, Anh Tuan; Nguyen, M. Hong; Nguyen, Long Tien; Trinh, Lai Thi; Tran, Dieu-Thuy Thi; Ta, Sang Viet; Hoshikawa, Ken; Sugimoto, Koh‐ichi; Ezura, Hiroshi

doi:10.1007/s10681-021-02853-5

Cited by 6 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HK cultivar has been widely used as a model commercial tomato variety in recent studies (Vu et al 2020b;Tran et al 2021) due to its higher genetic transformation e ciency and integration of T-DNA in the genome. Out of 10 lines tested, ET1 and ET5 exhibited transformation e ciencies comparable to the HK.…”

Section: Resultsmentioning

confidence: 99%

“…However, e cient modi cation of these parthenocarpy-related genes in commercial tomato cultivars is still limited. SlAGL6 was successfully mutated in M82 cultivar to induce parthenocarpy (Klap et al 2017), while seedless phenotype was observed only in Micro-Tom sliaa9 edited lines (Saito et al 2011), but not in M82 and Ailsa Craig (AC) backgrounds (Tran et al 2021; Ueta et al 2017). The sliaa9 parthenocarpic breeding lines were generated by crossing gene-edited Micro-Tom and inbred tomato lines.…”

Section: Introductionmentioning

confidence: 99%

“…The sliaa9 parthenocarpic breeding lines were generated by crossing gene-edited Micro-Tom and inbred tomato lines. Although the mutant allele was adopted, only 35.8% of fruits in the T 4 population were seedless, and the fruit weight was signi cantly reduced (Tran et al 2021). Slagl6/M82 (sg), on the other hand, produced around 80% of seedless fruits in the G1 population (Klap et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…The use of pEgP237-gRNA2(20b)-2A-GFP resulted in a 100% mutation rate in Micro-Tom shoots but only 42.9% in AC shoots. Unlike Micro-Tom, AC edited plants were not reported to produce parthenocarpic fruits(Tran et al 2021;Ueta et al 2017). Table…”

mentioning

confidence: 99%

See 3 more Smart Citations

Generation of parthenocarpic tomato plants in multiple elite cultivars using the CRISPR/Cas9 system

Nguyen,

Van Vu,

Shelake

et al. 2024

Mol Breeding

View full text Add to dashboard Cite

Tomato (Solanum lycopersicum L.) is one of the most important crops in the world for its fruit production. Advances in cutting-edge techniques have enabled the development of numerous critical traits related to the quality and quantity of tomatoes. Genetic engineering techniques, such as gene transformation and gene editing, have emerged as powerful tools for generating new plant varieties with superior traits. In this study, we induced parthenocarpic traits in a population of elite tomato (ET) lines. At rst, the adaptability of ET lines to genetic transformation was evaluated to identify the best-performing lines by transforming the SlANT1 gene overexpression cassette and then later used to produce the SlIAA9 knockout lines using the CRISPR/Cas9 system. ET5 and ET8 emerged as excellent materials for these techniques and showed higher e ciency. Typical phenotypes of knockout sliaa9 were clearly visible in G0 and G1 plants, in which simple leaves and parthenocarpic fruits were observed. The high e ciency of the CRISPR/Cas9 system in developing new tomato varieties with desired traits in a short period was demonstrated by generating T-DNA-free homozygous sliaa9 knockout plants in the G1 generation. Additionally, a simple arti cial fertilization method was successfully applied to recover seed production from parthenocarpic plants, securing the use of these varieties as breeding materials.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Generation of parthenocarpic tomato plants in multiple elite cultivars using the CRISPR/Cas9 system

Nguyen,

Van Vu,

Shelake

et al. 2024

Mol Breeding

View full text Add to dashboard Cite

show abstract

“…However, rin reduced nutritional components, flavour and colour of the fruits but PL gene had recorded otherwise [77,78]. Tomato mutant iaa9-3 line is capable of developing parthenocarpic variety that would be seedless and of high quality [79]. Mutation resulted in generating heat-tolerant tomatoes which exhibited high pollen fertility and fruit set [80,81].…”

Section: Mutationmentioning

confidence: 99%

Perspective Chapter: Accelerating Demand-Led Tomato Breeding for Emerging Markets in Africa

Adu-Dapaah¹,

Osei²,

Adjebeng‐Danquah³

et al. 2022

Tomato - From Cultivation to Processing Technology

View full text Add to dashboard Cite

Tomato production in Africa has increased due to increased population, rising consumer demands for nutritious and healthy food and potential use of improved technologies. Demand-led’ plant breeding puts producers and consumers at the heart of research and development involving stakeholders even before the research starts. These ‘stakeholders’ are not only farmers but key actors along the tomato value chain. They influence how the tomato is traded as: fresh food and processing product. This chapter focuses on different approaches to fast-track tomato breeding so as to contribute to the transformation of African agriculture by enabling small scale farmers to compete in local and regional markets, by increasing the availability and adoption of high performing tomato varieties that meet market demands. It further outlines development of varieties that meet farmer needs, consumer preferences, and market demand in Africa. These new varieties are designed to meet client needs by connecting plant breeders with crop value chains, seed distribution organizations, and encouraging enterprise and entrepreneurship in transforming agriculture in Africa. Lastly, it outlines the prospects and challenges associated with demand-led breeding of tomato and offers suggestions to increase food security in Africa.

show abstract