Developing <i>in vitro</i> assays to quantitatively evaluate the interactions of dressings with wounds

Ngo, Qiaowei; Anand, Padmaja; Wang, Yingting; Ananthanarayanan, Abhishek; Gong, Ping; Newby, Craig S.; Mcmillian, Michael M.

doi:10.1111/wrr.12746

Cited by 4 publications

(3 citation statements)

References 6 publications

(14 reference statements)

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“…Cell migration into these voids can be monitored by microphotography after staining. In addition to the assay of test sample extracts, WDs can be assayed in both the wound-scratch and in the 3D model by direct application to a monolayer with a scratch [203] or to a 3D collagen matrix [204].…”

Section: Prm17mentioning

confidence: 99%

Polyhexanide-Releasing Membranes for Antimicrobial Wound Dressings: A Critical Review

Guiomar

Urbano

2022

Membranes

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The prevalence of chronic, non-healing skin wounds in the general population, most notably diabetic foot ulcers, venous leg ulcers and pressure ulcers, is approximately 2% and is expected to increase, driven mostly by the aging population and the steady rise in obesity and diabetes. Non-healing wounds often become infected, increasing the risk of life-threatening complications, which poses a significant socioeconomic burden. Aiming at the improved management of infected wounds, a variety of wound dressings that incorporate antimicrobials (AMDs), namely polyhexanide (poly(hexamethylene biguanide); PHMB), have been introduced in the wound-care market. However, many wound-care professionals agree that none of these wound dressings show comprehensive or optimal antimicrobial activity. This manuscript summarizes and discusses studies on PHMB-releasing membranes (PRMs) for wound dressings, detailing their preparation, physical properties that are relevant to the context of AMDs, drug loading and release, antibacterial activity, biocompatibility, wound-healing capacity, and clinical trials conducted. Some of these PRMs were able to improve wound healing in in vivo models, with no associated cytotoxicity, but significant differences in study design make it difficult to compare overall efficacies. It is hoped that this review, which includes, whenever available, international standards for testing AMDs, will provide a framework for future studies.

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Section: Prm17mentioning

confidence: 99%

Polyhexanide-Releasing Membranes for Antimicrobial Wound Dressings: A Critical Review

Guiomar

Urbano

2022

Membranes

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show abstract

Section: Biological Evaluation: Wound Healingmentioning

confidence: 99%

Novel Polyhexanide-Releasing Membranes for Antimicrobial Wound Dressings: A Critical Review

Guiomar¹,

Urbano²

2022

Preprint

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The prevalence for chronic, non-healing skin wounds in the general population, most notably diabetic foot ulcers, venous leg ulcers and pressure ulcers, is approximately 2% and is expected to increase, driven mostly by an aging population and the steady rise in obesity and diabetes. Non-healing wounds often become infected, increasing the risk of life-threatening complications, which poses a significant socioeconomic burden. Aiming at an improved management of infected wounds, a variety of wound dressings incorporating antimicrobials (AMDs), namely polyhexanide (poly(hexamethylene biguanide); PHMB), have been introduced in the wound care market. However, many wound care professionals agree that none shows comprehensive and optimal antimicrobial activity. This manuscript summarizes and discusses studies on novel PHMB-releasing membranes (PRMs) for wound dressings, detailing their preparation, physical properties relevant in the context of AMDs, drug loading and release, antibacterial activity, biocompatibility, wound healing capacity, and clinical trials conducted. Some of these PRMs were able to improve wound healing in in vivo models, with no associated cytotoxicity, but significant differences in study design make it difficult to compare overall effi-cacies. It is hoped that this review, which includes, whenever available, international standards for testing AMDs, will provide a framework for future studies.their preparation, physical properties relevant in the context of AMDs, drug loading and release, antibacterial activity, biocompatibility, wound healing capacity, and clinical trials conducted. Some of these PRMs were able to improve wound healing in in vivo models, with no associated cytotoxicity, but significant differences in study design make it difficult to compare overall efficacies. It is hoped that this review, which includes, whenever available, international standards for testing AMDs, will provide a framework for future studies.

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“…Appropriate wound dressings are essential for minimizing the risk of infection and for facilitating optimal wound healing (12). Hydrogels are defined as suitable for all stages of the healing process of a wide range of wounds because they are non-irritant, non-reactive with biological tissue and ideally permeable to metabolites (13).…”

Section: Introductionmentioning

confidence: 99%

Wound healing and anti-oxidant activities induced by an alginate hydrogel-based ointment

Cabrera,

Greif,

Giusti

et al. 2023

Braz. J. Hea. Rev.

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Background: Alginate, a natural polysaccharide derived from seaweed, has shown to be better than other modern dressings in reducing wound area. The Brazilian population and medical care staff have used hydrogel-based ointment containing alginate for years in treatment of acute and chronic wounds. It is popularly known for their benefits, but so far, no molecular and cellular scientific proof of their wound healing capability has been tested. Objective: Since there is little information about the molecular and cellular responses of the hydrogel-based ointment containing alginate to improve wound healing mechanisms, in this study we evaluate the wound healing capability of a specific hydrogel employing in vitro models of human skin keratinocytes and fibroblastos. Methods: HaCaT-SF and HDFn cells were used as models of human skin keratinocytes and fibroblasts, respectively. The ointment effects in the wound healing were evaluated specifically on cell proliferation and migration, analyzing gene expression profiles and soluble factors secretion, as well as antioxidant activity. Results: From the analysis several genes related to the wound healing stages showed differential expression and secretion patterns in ointment-treated samples compared with control ones. Among these, cytokines and chemokines (involved in inflammation); growth factors and angiogenesis related proteins (implied in cell proliferation); matrix metalloproteinases and serine protease inhibitors (engaged in cell migration and tissue remodeling) were identified. Furthermore, this hydrogel exhibited anti-oxidant properties against oxidative damage in keratinocytes. Conclusions: Overall, our results indicate that this hydrogel-based ointment may function as a modulator of the wound healing process, promoting homeostasis and correct tissue repair.

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Developing in vitro assays to quantitatively evaluate the interactions of dressings with wounds

Cited by 4 publications

References 6 publications

Polyhexanide-Releasing Membranes for Antimicrobial Wound Dressings: A Critical Review

Polyhexanide-Releasing Membranes for Antimicrobial Wound Dressings: A Critical Review

Novel Polyhexanide-Releasing Membranes for Antimicrobial Wound Dressings: A Critical Review

Wound healing and anti-oxidant activities induced by an alginate hydrogel-based ointment

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