Developing biological resource banks as a supporting tool for wildlife reproduction and conservation

León–Quinto, Trinidad; Simón, Miguel Ángel; Cadenas, Rafael; Jones, Jimmy; Martínez-Hernández, Francisco; Moreno, Juan Manuel; Vargas, Astrid; Martínez, Fernando; Soria, Bernat

doi:10.1016/j.anireprosci.2008.05.070

Cited by 65 publications

(52 citation statements)

References 66 publications

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“…Assim, entre as estratégias de conservação dessa espécie, a criopreservação de germoplasma pela preservação de tecido somático derivado da pele consiste em uma alternativa interessante, uma vez que permite maior facilidade na obtenção e ampla amostragem por população (León-Quinto et al, 2009). Além disso, tecidos somáticos criopreservados são fontes importantes de células doadoras de núcleo para a transferência nuclear ou clonagem.…”

Section: Introductionunclassified

Caracterização histomorfológica do sistema tegumentar auricular de cateto - Pecari tajacu Linnaeus, 1758)

Borges

Bezerra

Costa

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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RESUMO A criopreservação de tecido somático derivado da pele de catetos consiste numa alternativa para a conservação da biodiversidade por meio da associação com a transferência nuclear. Nesse contexto, a manipulação de tecidos da pele é uma etapa crucial para o sucesso dessa biotécnica. Portanto, o objetivo do presente estudo, foi caracterizar o sistema tegumentar auricular periférico de catetos, visando aprimorar a conservação tecidual. Para tanto, fragmentos auriculares de oito animais foram avaliados quanto às camadas teciduais, aos componentes, à atividade proliferativa e à viabilidade metabólica, usando-se as colorações hematoxilina-eosina e tricrômico de Gomori, quantificação de AgNORs e microscopia eletrônica de transmissão. Assim, tamanhos de 104,2µm e 222,6µm foram observados para epiderme e derme, com uma proporção volumétrica de 36,6% e 58,7%, respectivamente. Além disso, na epiderme, foram evidenciadas as camadas basal (22,5µm), intermediárias (53,5µm) e córnea (28,2µm), com valores médios de 65,3 células epidermais, 43,4 melanócitos e 14,8 halos perinucleares. Já a derme apresentou 127 fibroblastos, com 2,5 AgNORs/nucléolo. Adicionalmente, a atividade metabólica foi de 0,243. Em conclusão, o sistema tegumentar auricular periférico de catetos possui algumas marcantes variações em relação a outros mamíferos, quanto ao número de camadas e espessura da epiderme, quantidade de células epidermais, melanócitos e parâmetros proliferativos.

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Section: Introductionunclassified

Caracterização histomorfológica do sistema tegumentar auricular de cateto - Pecari tajacu Linnaeus, 1758)

Borges

Bezerra

Costa

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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“…Nowadays, examples of application of postmortem sperm collection from the epididymis for the conservation of endangered species as in ibex (Santiago-Moreno et al, 2006) and in constructing semen banks (Ehling et al, 2006;Bagirov et al, 2009;León-Quinto et al, 2009) are reported. However, sometimes, epididymal sperm extraction is not easily practicable in marginal regions, owing to the lack of facilities and expertise near the farming areas of local breeds.…”

Section: Introductionmentioning

confidence: 99%

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Turri

Madeddu

Gliozzi

et al. 2014

Animal

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The interest to develop assisted reproductive technologies and cryobanking for farm animal genetic resource conservation has recently increased. However, cryopreservation for ex-situ management of genetic diversity sometimes is not routinely feasible, owing to the lack of facilities (AI centres, laboratories) and expertise near the local breed farming area. In these cases, epididymal sperm obtained from slaughtered or castrated animals, associated with the possibility of managing rather long periods between animal death, sperm recovery and freezing, would increase the opportunities to create semen storages. This investigation addresses the pre-freeze/post-thaw quality of goat epididymal sperm as a function of testicle storage temperature (environment or +5°C) and time elapsed between animal's death and sperm recovery (0, 24, 48, 72 h) to establish the optimal protocols for the recovery and cryopreservation of epididymal sperm in this species. Testicles of 50 mature bucks collected at the abattoir were divided in two groups: half of the testicles (n = 50) were transported to the laboratory at environment temperature (E), whereas the remaining half (n = 50) at a refrigeration temperature (R) of +5°C. In the two groups (E) and (R), one testicle from each pair was processed after slaughter forming the time 0 groups (0E and 0R). The contralateral testicle was processed after 24, 48 or 72 h of storage, at the corresponding temperature. Sperm motility and kinetic parameters, viability and morphology were assessed in pre-freeze and post-thaw samples. Until 48 h postmortem, both E and R temperatures are able to maintain good pre-freeze epididymal sperm quality. After 48 h postmortem, R temperature is fundamental to reduce epididymal sperm quality decay in pre-freeze samples. Moreover, testicle refrigeration also has a positive impact on post-thaw samples, allowing a lower decline through time considering total motility, kinetics parameters, sperm viability and sperm abnormalities. Therefore, when sperm cryopreservation is not immediately practicable, goat testicles should be transported and stored at 5°C up to a maximum of 48 h postmortem to ensure an acceptable sperm quality.

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“…Maintaining and, if necessary, increasing biodiversity is the prime aim of conservation research (Leon-Quinto et al 2009;Sutherland et al 2009). Three conservation measures (in situ, ex situ conservation, and captive breeding) have been adopted to maintain and restore porpoise populations since the early 1990s by a Research Team of Conservation Biology of Aquatic Animals at the Institute of Hydrobiology, Chinese Academy of Sciences (Wang 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Three conservation measures (in situ, ex situ conservation, and captive breeding) have been adopted to maintain and restore porpoise populations since the early 1990s by a Research Team of Conservation Biology of Aquatic Animals at the Institute of Hydrobiology, Chinese Academy of Sciences (Wang 2009). As a complementary supporting program to reinforce these three strategies, it is of significant scientific importance to establish a biological resources bank (Wiidt 1992;Holt and Pickard 1999;Comizzoli et al 2000;Leon-Quinto et al 2009), such as, the preservation of individual animals, semen, embryos, blood, tissue, cells, genomic libraries, and cDNA libraries. One of the most effective approaches is the establishment of somatic cell lines using cryopreservation (Shi 1989;Li et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise

Wang

Nie

et al. 2011

In Vitro Cell.Dev.Biol.-Animal

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The Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis), as the sole freshwater subspecies of N. phocaenoides, is endemic to the Yangtze River and its adjacent lakes. Its population has declined significantly over recent decades. In this study, we established a skin-derived finite fibroblast cell line of the Yangtze finless porpoise, named YFP-SF1, using primary cell culture methods, and an immortalized cell line, T-YFP-SF1, through co-transfection (GFP and SV40 T antigens) techniques. YFP-SF1 proliferated continuously with a minimum population doubling time of 31 h and exhibited age-dependent changes in growth rate. T-YFP-SF1 cells exhibited fibroblast morphology and were characterized by a shorter doubling time, higher attachment efficiencies, colony formation at a low seeding density, and growth in low serum concentrations. Anchorage independence and foci formation in the cell monolayer were observed from passage 36. The chromosome number of YFP-SF1 and T-YFP-SF1 remained stable at 2n=44 in the early passages, and the viability of thawed cells remained above 90% after cryopreservation in liquid nitrogen. Taken together, we have established fibroblast cell lines of Yangtze finless porpoise for the first time, which might assist as an in vitro model for this endangered mammal.

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Developing biological resource banks as a supporting tool for wildlife reproduction and conservation

Cited by 65 publications

References 66 publications

Caracterização histomorfológica do sistema tegumentar auricular de cateto - Pecari tajacu Linnaeus, 1758)

Caracterização histomorfológica do sistema tegumentar auricular de cateto - Pecari tajacu Linnaeus, 1758)

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise

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