Developing Antibiofilm Fibrillar Scaffold with Intrinsic Capacity to Produce Silver Nanoparticles

Abstract

The development of biomedical systems with antimicrobial and antibiofilm properties is a difficult medical task for preventing bacterial adhesion and growth on implanted devices. In this work, a fibrillar scaffold was produced by electrospinning a polymeric organic dispersion of polylactic acid (PLA) and poly(α,β-(N-(3,4-dihydroxyphenethyl)-L-aspartamide-co-α,β-N-(2-hydroxyethyl)-L-aspartamide) (PDAEA). The pendant catechol groups of PDAEA were used to reduce silver ions in situ and produce silver nanoparticles onto the surface of the electrospun fibers through a simple and reproducible procedure. The morphological and physicochemical characterization of the obtained scaffolds were studied and compared with virgin PLA electrospun sample. Antibiofilm properties against Pseudomonas aeruginosa, used as a biofilm-forming pathogen model, were also studied on planar and tubular scaffolds. These last were fabricated as a proof of concept to demonstrate the possibility to obtain antimicrobial devices with different shape and dimension potentially useful for different biomedical applications. The results suggest a promising approach for the development of antimicrobial and antibiofilm scaffolds.

Antibacterial Broad‐Spectrum Dendritic/Gellan Gum Hybrid Hydrogels with Rapid Shape‐Forming and Self‐Healing for Wound Healing Application

“…Two types of bacteria were used in the study, S. aureus 2569 (Gram‐positive) and E. coli 178 (Gram‐negative). [ 37 ] The MIC and MBC values for the cationic dendrimer showed good antibacterial properties against both bacteria, Table 2 . The results also detailed that higher dendrimer concentrations were required to kill S. aureus than E. coli , suggesting greater efficiency against Gram‐ than Gram+ strains.…”

Schiff Base-Based Hydrogel Embedded with In Situ Generated Silver Nanoparticles Capped by a Hyaluronic Acid–Diethylenetriamine Derivative for Wound Healing Application

“…33 During the initial stages of bacterial infection, wounds become locally acidified, and pH-responsive hydrogels can produce an on-demand action, help minimize side effects, and improve the potential for more effective treatment. 34,35 Silver nanoparticles (AgNPs), well-known for their potent antibacterial properties, 20,36 have been also shown to stimulate anti-inflammatory responses and facilitate the various stages of the healing process. 37,38 The use of self-healing hydrogels loaded with antibacterial AgNPs proves to be advantageous to mitigate the toxicity concern and to enable sustained drug delivery, 39−42 acting as reservoirs for drugs and facilitating localized release at the target site.…”

“…This severe and progressive infection can develop phenomena of antibiotic resistance in time, making using antibacterial substances that do not induce antimicrobial resistance, such as silver nanoparticles, increasingly important . During the initial stages of bacterial infection, wounds become locally acidified, and pH-responsive hydrogels can produce an on-demand action, help minimize side effects, and improve the potential for more effective treatment. , Silver nanoparticles (AgNPs), well-known for their potent antibacterial properties, , have been also shown to stimulate anti-inflammatory responses and facilitate the various stages of the healing process. , The use of self-healing hydrogels loaded with antibacterial AgNPs proves to be advantageous to mitigate the toxicity concern and to enable sustained drug delivery, − acting as reservoirs for drugs and facilitating localized release at the target site. This approach reduces side effects and circumvents the challenges associated with systemic administration…”