Developing and physicochemical evaluation of cross-linked electrospun gelatin–glycerol nanofibrous membranes for medical applications

Morsy, Reda; Hosny, M.; Reicha, Fikry M.; Elnimr, Tarek

doi:10.1016/j.molstruc.2017.01.064

Cited by 42 publications

(15 citation statements)

References 24 publications

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“…Both of the crosslinked gelatin (curve b) and the gelatin/genipin (curve c) curves showed a similar trend of thermal stability: two weight loss peaks were observed in Figure and a rapid change in mass occurs in the range of 290°C‐400°C. All the samples showed an initial weight loss around 80°C as a result of the vaporization of the moisture in the electrospun nanofibrous constructs upon heating…”

Section: Resultsmentioning

confidence: 99%

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Storage stability of electrospun pure gelatin stabilized with EDC/Sulfo‐NHS

Ghassemi

Slaughter

2018

Biopolymers

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With the rapid development of biomimetic polymers for cell-based assays and tissue engineering, crosslinking electrospun nanofibrous biopolymer constructs is of great importance for achieving sustainable and efficient three-dimensional scaffold constructs. Uncrosslinked electrospun gelatin nanofibrous constructs immediately and completely dissolved in aqueous solutions due to their aqueous solubility and poor storage stability. Here, a novel and versatile approach for the fabrication and crosslinking of electrospun gelatin construct with tunable porosity and high aspect ratio nanofibers is presented. Uncrosslinked electrospun gelatin/genipin nanofibrous and pure gelatin nanofibrous constructs exhibited smooth surfaces that were well-defined, with a diameter in the range of 448 ± 364 nm and 257 ± 57 nm, respectively. Dehydrothermal, genipin-EDC/Sulfo-NHS, and EDC/Sulfo-NHS crosslinking approaches were examined to achieve insoluble gelatin nanofibrous constructs that were suitable for cell-based assays. Mechanical characterization demonstrated that the pure gelatin nanofibrous construct crosslinked via EDC/Sulfo-NHS exhibited an increased mechanical strength and stiffness and showed no dissolution in aqueous solutions and retained its fiber morphology. An excellent 1 month storage stability was demonstrated at 22, 4, -20, and -80°C (dehydrated) and at 4°C (hydrated). The as-crosslinked gelatin nanofibrous construct was highly biocompatible (90% cell viability), as demonstrated by the promoted proliferation of PC12 cells.

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Section: Resultsmentioning

confidence: 99%

“…5 and a rapid change in mass occurs in the range of 290 °C – 400 °C. All the samples showed an initial weight loss around 80 °C as a result of the vaporization of the moisture in the electrospun nanofibrous constructs upon heating [28].…”

Section: Resultsmentioning

confidence: 99%

Storage stability of electrospun pure gelatin stabilized with EDC/Sulfo‐NHS

Ghassemi

Slaughter

2018

Biopolymers

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“…The amide I of all edible films with the strong absorbance feature was mainly connected with C=O stretching vibration, which was related to coupling to CN stretching, CCN deformation, and the contribution of in-plane NH bending modes 35 . C–N stretching was in the range of 1544–1551 cm −1 (amide II), C–N stretching and N-H bending were a combination band, CH 2 bending (scissors) vibration and stretching were at 1438–1453 cm −1 , CH 2 stretching was at 1378–1394 cm −1 , and C–N stretching and N–H bending were at 1229–1236 cm −1 (amide III) 36 . The amplitude of the peaks varied with the uniform mixing of the gelatin/chitosan edible film in different ways 37 .…”

Section: Discussionmentioning

confidence: 98%

Characterization of gelatin/chitosan ploymer films integrated with docosahexaenoic acids fabricated by different methods

Cai

Shi

et al. 2019

Sci Rep

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In this study, docosahexaenoic acid powder-enhanced gelatin-chitosan edible films were prepared by casting, electrospinning and coaxial electrospinning, respectively. The color (CR), transparency (UV), light transmission (UV), mechanical strength (TA-XT), thermal stability (DSC), crystalline structures (XRD), molecular interactions (FTIR), and microstructure (SEM) were assessed in the analytical research. The results of the research showed that the electrospinning process and the coaxial electrospinning process produced a smooth surface visible to by the naked eye and a uniform granular network structure in a unique film-forming manner, thereby exhibiting good water solubility and mechanical properties. In contrast, the casted film was smooth, transparent, and mechanically strong but poorly water soluble. It was also found that the addition of docosahexaenoic acid powder affected the optical, physical and mechanical properties of the film to varying degrees.

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“…The nearly invisible peak corresponding to amide III (around 1240 cm −1 ) [ 39 ] associated with C–N stretching and N–H bending from dried samples suggests a change in secondary structure of gelatin owing to hydrogen bonding and the conformation of protein structure. The same observation regarding to the lack of amide III band in gelatin/glycerol electrospun fibers has been made by Reda Morsy et al [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment and Characterization of Some New Photosensitizers for Antimicrobial Photodynamic Therapy (aPDT)

et al. 2020

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The novelty of this study consists on the formulation and evaluation of five complex experimental natural photosensitizers (PS): gel with oregano essential oil (O), gel with methylene blue (AM), gel with a mixture of essential oils (Thieves-H), gel with arnica oil and curcuma extract (CU) and gel with frankincense essential oil (T), used as photosensitizing agents (PS) in antimicrobial photodynamic therapy (aPDT) in the control of microbial biofilm in oral cavity. The experimental PS were characterized by gas chromatography-mass spectrometry (GC-MS), Fourier-transform infrared spectroscopy (FTIR), UV-Vis spectroscopy, cytotoxicity assay, antimicrobial effect and scanning electron microscopy (SEM). The IR spectra of the experimental PS with essential oils exhibit absorption bands due to the presence of water and glycerol in high quantities. The studied compounds had a reduced cytotoxic effect on cell cultures. The lowest cytotoxic effect was observed in experimental PS with oregano essential oil and methylene blue PS. Essential oils with proven antibacterial capabilities used in experimental PS confer antibacterial activity to the gels in which they are incorporated, an activity that may be more efficient use of a PDT therapy. Single bacteria were detected mainly by SEM after 12 h, while aggregate bacteria and micro colonies dominated the samples at 48 h.

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Developing and physicochemical evaluation of cross-linked electrospun gelatin–glycerol nanofibrous membranes for medical applications

Cited by 42 publications

References 24 publications

Storage stability of electrospun pure gelatin stabilized with EDC/Sulfo‐NHS

Storage stability of electrospun pure gelatin stabilized with EDC/Sulfo‐NHS

Characterization of gelatin/chitosan ploymer films integrated with docosahexaenoic acids fabricated by different methods

Assessment and Characterization of Some New Photosensitizers for Antimicrobial Photodynamic Therapy (aPDT)

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