Developing an SCAR and ITS reliable multiplex PCR-based assay for safflower adulterant detection in saffron samples

Evaluation of saffron (Crocus sativus L.) adulteration with plant adulterants by 1H NMR metabolite fingerprinting

“…Also, the UV-Vis spectrophotometric method proposed by ISO 3632-2 (ISO, 2010) may not detect saffron contamination with amounts of up to 20% (w/w) of safflower or turmeric, as it was recently reported (Sabatino, Scordino, Gargano, Belligno, Traulo, & Gagliano, 2011). For the detection of plant adulterants in saffron, several chromatographic (Alonso, Salinas, & Garijo, 1998;Haghighi, Feizy, & Hemati Kakhki, 2007;Lozano, Castellar, Simancas, & Iborra, 1999;Sabatino et al, 2011;Sampathu, Shivashankar, Lewis, & Wood, 1984) and molecular (Babaei, Talebi, & Bahar, 2014;Javanmardi, Bagheri, Moshtaghi, Sharifi, & Hemati Kakhki, 2011;Ma, Zhu, Li, Dong, & Tsim, 2001;Marieschi, Torelli, & Bruni, 2012;Torelli et al, 2014) methods have been employed so far with encouraging results. The use of DNA markers has allowed the detection of low amounts (up to 1%) of several bulking materials including safflower and turmeric (Javanmardi et al, 2011;Marieschi et al, 2012).…”

A simplified Protocol to Induce Callogenesis in Protoplasts of Date Palm (Phoenix dactylifera L.) Cultivars

“…DNA barcoding on the other hand that takes advantage of using particular nuclear or organellar genome sequences as genetic markers seems a way forward in determination any impurities. This technique is rapid, sensitive, accurate and simple and in our case is efficient for species identification (16)(17)(18).…”

DNA Barcoding for Minor Crops and Food Traceability

“…This phenomenon implies a major risk for species substitution or uncontrolled admixture of manufactured plant products. Substitution or adulteration can be deliberate (e.g., to maximize financial gains) or inadvertent (e.g., due to an insufficient knowledge by farmers) but they can have serious consequences for consumers at any rate [14][15][16][17][18][19].…”