Developing an Excitation-Emission Matrix Fluorescence Spectroscopy Method Coupled with Multi-way Classification Algorithms for the Identification of the Adulteration of Shanxi Aged Vinegars

Peng, Tian-Qin; Yin, Xingtian; Sun, Weiqing; Ding, Baomiao; Ma, Li; Gu, Hui‐Wen

doi:10.1007/s12161-019-01586-5

Cited by 36 publications

(8 citation statements)

References 32 publications

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“…The spectra could be characterized by strong intensity peaks in 300–600 nm region. Phenolic compounds are designated as having fluorescent properties, and this range corresponds to the intensity due to these compounds [ 23 , 34 ]. The peak at around 470–500 nm was attributed to brown pigments, which can be produced by acetic acid bacteria [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

“…Spectroscopic methods were also used in differentiating mixtures of vinegars and, as an example, detection of the adulteration of sherry vinegars with molasses, rice, cider and wine vinegars was investigated with laser diode fluorescence spectroscopy in conjunction with chaotic algorithms [ 22 ]. Excitation–emission fluorescence spectroscopy, on the other hand, was used in differentiating authentic Shanxi aged vinegars from this vinegar mixed with acetic acid in combination with chemometric methods, and a 100% discrimination was achieved [ 23 ]. Although there have been many studies focusing on the different aspects of vinegar authentication, the number of studies on the detection of spirit vinegar and synthetic acetic acid is limited.…”

Section: Introductionmentioning

confidence: 99%

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Applications of UV–Visible, Fluorescence and Mid-Infrared Spectroscopic Methods Combined with Chemometrics for the Authentication of Apple Vinegar

Cavdaroglu

Özen

2023

Foods

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Spectroscopic techniques as untargeted methods have great potential in food authentication studies, and the evaluation of spectroscopic data with chemometric methods can provide accurate predictions of adulteration even for hard-to-identify cases such as the mixing of vinegar with adulterants having a very similar chemical nature. In this study, we aimed to compare the performances of three spectroscopic methods (fluorescence, UV–visible, mid-infrared) in the detection of acetic-acid/apple-vinegar and spirit-vinegar/apple-vinegar mixtures (1–50%). Data obtained with the three spectroscopic techniques were used in the generation of classification models with partial least square discriminant analysis (PLS-DA) and orthogonal partial least square discriminant analysis (OPLS-DA) to differentiate authentic and mixed samples. An improved classification approach was used in choosing the best models through a number of calibration and validation sets. Only the mid-infrared data provided robust and accurate classification models with a high classification rate (up to 96%), sensitivity (1) and specificity (up to 0.96) for the differentiation of the adulterated samples from authentic apple vinegars. Therefore, it was concluded that mid-infrared spectroscopy is a useful tool for the rapid authentication of apple vinegars and it is essential to test classification models with different datasets to obtain a robust model.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Applications of UV–Visible, Fluorescence and Mid-Infrared Spectroscopic Methods Combined with Chemometrics for the Authentication of Apple Vinegar

Cavdaroglu

Özen

2023

Foods

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“…Fluorescence Excitation Emission Matrix (EEM) spectroscopy has found many applications in the analysis of e.g. food samples, [1][2][3][4][5] machinery fluids, 6,7 environmental samples [8][9][10] such as water [11][12][13][14][15] and soil 10,16 , and even neutrino scintillator liquids. 17 EEM spectroscopy is usually performed by scanning a light sourceusually a broadband lamp coupled to a monochromatorand collecting the fluorescence using either an array detector or a second monochromator and broadband detector.…”

Section: Introductionmentioning

confidence: 99%

Rapid Fluorescence EEM spectra acquisition using super-cycle Hadamard-Transform Multiplexing

Ferguson

Loock

2023

Preprint

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Hadamard-transform multiplexing has recently been applied to increasingly complex spectroscopic techniques. It had been shown that the data acquisition time for fluorescence Excitation-Emission-Matrix spectroscopy can be reduced by one or two orders of magnitude using Hadamard-Transform multiplexing of the excitation light using a programmable lightsource. In these previous studies, the data acquisition rate had been limited by the time it took to record an EEM, i.e. to complete one cycle of multiplexed excitation spectra. The extraction of chemical information, such as concentration and chemical identity, is then obtained from parallel factor (PARAFAC) analysis of the sequence of EEM spectra. In this contribution we increase the data acquisition rate by another order of magnitude, i.e. to the time it takes to record a single excitation spectrum. Our algorithm is entirely based on improved data processing, i.e. it can be applied to previously recorded Hadamard-Transform multiplexed data sets. The algorithm is based on three previously unexplored approaches: (1) we perform a PARAFAC multivariate analysis on the raw (multiplexed) data set (2) the PARAFAC loading vectors are obtained prior to obtaining the score vectors, (3) when loading vectors are difficult to obtain from the stack of EEM spectra, we instead use a rolling-average approach to considerably increase the number of spectra and the stability of the fit. Analysis of experimental data shows that fluorescence EEM spectra with 7 excitation wavelengths and over 1000 emission wavelengths can be obtained in less than 20 ms.

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“…Excitation–emission matrix (EEM) fluorescence spectroscopy can provide complete fluorescence information on measured samples by covering a wide range of different excitation and emission wavelengths. In recent years, the EEM technique has been gaining widespread analytical acceptance in many fields such as environmental [ 2 ], cell culture media [ 3 ], cosmetics [ 4 ], food [ 5 , 6 ], and so on. For a given sample, a two-dimensional signal that contains peaks from all of the excited fluorophores can be obtained [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

New Constructed EEM Spectra Combined with N-PLS Analysis Approach as an Effective Way to Determine Multiple Target Compounds in Complex Samples

Zhu

Feng

et al. 2022

Molecules

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Excitation–emission matrix (EEM) fluorescence spectroscopy has been applied to many fields. In this study, a simple method was proposed to obtain the new constructed three-dimensional (3D) EEM spectra based on the original EEM spectra. Then, the application of the N-PLS method to the new constructed 3D EEM spectra was proposed to quantify target compounds in two complex data sets. The quantitative models were established on external sample sets and validated using statistical parameters. For validation purposes, the obtained results were compared with those obtained by applying the N-PLS method to the original EEM spectra and applying the PLS method to the extracted maximum spectra in the concatenated mode. The comparison of the results demonstrated that, given the advantages of less useless information and a high calculating speed of the new constructed 3D EEM spectra, N-PLS on the new constructed 3D EEM spectra obtained better quantitative analysis results with a correlation coefficient of prediction above 0.9906 and recovery values in the range of 85.6–95.6%. Therefore, one can conclude that the N-PLS method combined with the new constructed 3D EEM spectra is expected to be broadened as an alternative strategy for the simultaneous determination of multiple target compounds.

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Developing an Excitation-Emission Matrix Fluorescence Spectroscopy Method Coupled with Multi-way Classification Algorithms for the Identification of the Adulteration of Shanxi Aged Vinegars

Cited by 36 publications

References 32 publications

Applications of UV–Visible, Fluorescence and Mid-Infrared Spectroscopic Methods Combined with Chemometrics for the Authentication of Apple Vinegar

Applications of UV–Visible, Fluorescence and Mid-Infrared Spectroscopic Methods Combined with Chemometrics for the Authentication of Apple Vinegar

Rapid Fluorescence EEM spectra acquisition using super-cycle Hadamard-Transform Multiplexing

New Constructed EEM Spectra Combined with N-PLS Analysis Approach as an Effective Way to Determine Multiple Target Compounds in Complex Samples

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