Developing an electrochemical sensor for the<i>in vivo</i>measurements of dopamine

Delmo, Naela; Mostafiz, Bahar; Ross, Ashley E.; Suni, Johanna; Peltola, Emilia

doi:10.1039/d2sd00230b

Cited by 7 publications

(2 citation statements)

References 262 publications

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“…Although sensitive and selective detection of DA in the presence of the interfering species is possible using a screen-printed electrode (SPE) or glassy carbon (GC) electrode, detecting dopamine in attoliter droplets using an SPE or GC electrode is challenging due to the large capacitive current, which is proportional to the electrode area [48][49][50][51]. With UME having a micron-scale electroactive area, the dopamine in the attoliter vesicle can be detected.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Characterization of Neurotransmitters in a Single Submicron Droplet

Park,

Park

2024

Biosensors

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Single-entity electrochemistry, which employs electrolysis during the collision of single particles on ultramicroelectrodes, has witnessed significant advancements in recent years, enabling the observation and characterization of individual particles. Information on a single aqueous droplet (e.g., size) can also be studied based on the redox species contained therein. Dopamine, a redox-active neurotransmitter, is usually present in intracellular vesicles. Similarly, in the current study, the electrochemical properties of neurotransmitters in submicron droplets were investigated. Because dopamine oxidation is accompanied by proton transfer, unique electrochemical properties of dopamine were observed in the droplet. We also investigated the electrochemical properties of the adsorbed droplets containing DA and the detection of oxidized dopamine by the recollision phenomenon.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Characterization of Neurotransmitters in a Single Submicron Droplet

Park,

Park

2024

Biosensors

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“…Furthermore, studies have shown that more complex nanostructures are less susceptible to surface fouling [39,40]. However, with the rapid E2 signalling on neurons [41] like DA, the temporal and spatial resolution alongside biocompatibility must be considered for in vivo measurements in the brain [42]. Recently, E2 was characterized using fast-scan cyclic voltammetry (FSCV) [43] and co-detection with DA was achieved with a modi ed waveform [44] which satis ed the temporal and spatial requirements for both compounds.…”

Section: Introductionmentioning

confidence: 99%

Key Role of Adsorption Site Abundance in the Direct Electrochemical Co-Detection of Estradiol and Dopamine

Delmo,

Pande,

Peltola

2024

Preprint

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Estradiol (E2) is a hormone that influences various aspects of women’s health. Beyond its reproductive functions, E2 impacts neurotransmitter systems such as dopamine (DA). Vertically aligned carbon nanofibers (VACNFs) have shown good sensitivity, selectivity, biocompatibility, and reduced fouling in DA sensing. In this study, we explore the use of Ti-Ni-CNF electrodes with two fiber lengths (referred to as short and long) for the direct electrochemical co-detection of E2 and DA. In E2 detection, the electrodes demonstrate a wide linear range of 0.05-10 µM and sensitivity of 0.016 and 0.020 µA/µM for short and long CNFs, respectively. The sensor performance remains largely unaffected even in the presence of other steroid hormones such as progesterone and testosterone. Remarkably, co-detection of E2 and DA shows promising peak separation, sensitivity, and stability. Furthermore, in such a scenario where both analytes rely on adsorption for oxidation, the availability of adsorption sites emerges as a critical factor, highlighting the significant role of material morphology in its performance. While detecting small and fluctuating physiological concentrations remains a challenge, these findings can be used in choosing and fabricating electrode materials for more accurate and accessible continuous hormone measurements, including the possibility of multianalyte sensing platforms.

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Microinstrumentation for Brain Organoids

Patel,

Shetty,

Acha

et al. 2024

Adv Healthcare Materials

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Brain organoids are three‐dimensional aggregates of self‐organized differentiated stem cells that mimic the structure and function of human brain regions. Organoids bridge the gaps between conventional drug screening models such as planar mammalian cell culture, animal studies, and clinical trials. They can revolutionize the fields of developmental biology, neuroscience, toxicology, and computer engineering. Conventional microinstrumentation for conventional cellular engineering, such as planar microfluidic chips; microelectrode arrays (MEAs), and optical, magnetic, and acoustic techniques, have limitations when applied to 3D organoids, primarily due to their limits with inherently 2D geometry and interfacing. Hence, there is an urgent need to develop new instrumentation that is compatible with live cell culture techniques and with scalable 3D formats of relevance to organoids. This review discusses conventional planar approaches and emerging 3D microinstrumentation necessary for advanced organoid‐machine interfaces. Specifically, the article surveys recently developed microinstrumentation, including 3D printed and curved microfluidics, 3D and fast‐scan optical techniques, buckling and self‐folding microelectrode arrays, 3D interfaces for electrochemical measurements, and 3D spatially controllable magnetic and acoustic technologies relevant to two‐way information transfer with brain organoids. The article highlights key challenges that must be addressed for robust organoid culture and reliable 3D spatiotemporal information transfer.This article is protected by copyright. All rights reserved

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Developing an electrochemical sensor for thein vivomeasurements of dopamine

Abstract: Measurements inside the human body are complicated. Here, we provide a short introduction of the main requirements for the successful in vivo determination of dopamine concentrations, together with a discussion...

Cited by 7 publications

References 262 publications

Electrochemical Characterization of Neurotransmitters in a Single Submicron Droplet

Electrochemical Characterization of Neurotransmitters in a Single Submicron Droplet

Key Role of Adsorption Site Abundance in the Direct Electrochemical Co-Detection of Estradiol and Dopamine

Microinstrumentation for Brain Organoids

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