Developing a Glyoxal-Crosslinked Chitosan/Gelatin Hydrogel for Sustained Release of Human Platelet Lysate to Promote Tissue Regeneration

Abstract

The clinical application of human platelet lysate (HPL) holds promise for tissue regeneration, and the development of an efficient vehicle for its delivery is desired. Chitosan-based hydrogels are potential candidates, but they often exhibit weak mechanical properties. In this study, a chitosan/gelatin (CS-GE) hydrogel crosslinked by glyoxal was fabricated for sustained release of HPL. The influence of HPL on Hs68 fibroblast and human umbilical vein endothelial cell (HUVEC) culture was evaluated, and we found that supplementing 5% HPL in the medium could significantly improve cell proliferation relative to supplementing 10% fetal bovine serum (FBS). Moreover, HPL accelerated the in vitro wound closure of Hs68 cells and facilitated the tube formation of HUVECs. Subsequently, we fabricated CS-GE hydrogels crosslinked with different concentrations of glyoxal, and the release pattern of FITC-dextrans (4, 40 and 500 kDa) from the hydrogels was assessed. After an ideal glyoxal concentration was determined, we further characterized the crosslinked CS-GE hydrogels encapsulated with different amounts of HPL. The HPL-incorporated hydrogel was shown to significantly promote the proliferation of Hs68 cells and the migration of HUVECs. Moreover, the release pattern of transforming growth factor-β1 (TGF-β1) and platelet-derived growth factor-BB (PDGF-BB) from hydrogel was examined in vitro, demonstrating a sustained release profile of the growth factors. Finally, the chick chorioallantoic membrane assay revealed that HPL encapsulation in the hydrogel significantly stimulated angiogenesis in ovo. These results demonstrate the great potential of the crosslinked CS-GE hydrogel to serve as an effective delivery system for HPL to promote tissue regeneration.

SDF-1 Functionalized Hydrogel Microcarriers for Skin Flap Repair

“…Similar to the migration assay, the angiogenic ability of the MC group was stronger than that of the NC group ( P < 0.001). It has been reported that gelatin plays an important role in the formation of blood vessels, and gelatin as the main component of hydrogel systems has been widely used to promote angiogenesis. − Therefore, the good cellular compatibility of gelatin enhanced the proliferation and increased the viability of HUVECs, which likely influenced the cells angiogenic potential. Taken together, these findings showed that the MC@SDF-1@Mat microcarriers were capable of significantly improving the migration and tube formation of HUVECs in vitro, which provides a good basis to investigate the regenerative capacity of these SDF-1 functionalized microcarriers in vivo.…”

Enhanced angiogenic potential of adipose-derived stem cell sheets by integration with cell spheroids of the same source

“…The CAM specimens were photographed, and the blood vessels were quantified by measuring the capillary area and counting the capillary branch points using ImageJ. Moreover, the immunohistochemical staining of the CAM sections was performed using anti-CD31 antibody as described previously [ 22 ]. The CAM sections were photographed under a microscope, and the ratio of CD31-positive area was quantified using ImageJ.…”

Are Natural Compounds a Promising Alternative to Synthetic Cross-Linking Agents in the Preparation of Hydrogels?

“…When glyoxal comes in contact with the human organism, it exacerbates the development and progression of diabetic nephropathy. Moreover, it can contribute to the development of Alzheimer’s and Parkinson’s diseases [ 106 , 118 , 119 , 120 , 121 , 122 ]. The cross-linking reaction with glyoxal occurs according to a homobifunctional mechanism, i.e., a one-step reaction.…”