This paper deals with the influence of implemented quality and environmental management systems ISO 9000 and ISO 14000 on enterprise performance. The paper delivers original results and comments on realized and processed questionnaire research concerning approx. 700 companies operating through Czech and Slovak Republics, called "Adaptability of entrepreneurship", financed by Silesian University in Opava. The paper evaluates the difference in enterprise performances as an effect of implementation of ISO management systems. The performance of 4 groups of companies (no ISO implemented, ISO 9000 implemented, ISO 14000 implemented and both ISO 9000 and 14000 implemented) is compared. 14 criteria were selected for evaluation, which can be marked as enterprise sustainability aspects. Three hypotheses were tested. The results show visible improvement in performance and awareness of selected priorities at companies with implemented ISO management systems. Problematic and results are discussed and compared with similar researches published inprofessional literature.The most important results of the paper were presented