Develope Micro clonal -propagation protocol forOxytenanthera abyssinica A.Rich. Munroto large scale micro-propagation

Abstract

In Ethiopia, Oxytenanthera abyssinica A.Rich. Munro has varies economic importance. However, conventional propagation methods of O. abyssinica are generally inefficient due to their low multiplication rate, time consuming, labor intensive, and too costly. The objective of this study was to develop a protocol for mass micropropagation of O. abyssinica through seed culture. Murashige and Skoog (MS) medium augmented with 6-Benzylaminopurine (BAP) was used for shoot initiation and multiplication. For in vitro rooting, MS medium supplemented with 3-Indole –butric acid (IBA) was used. In shoot initiation experiment all viable seeds were proliferated in 5-7 days of culturing. In shoot multiplication at 0.004 g/L BAP was Sucssefuly shoot multiplied, also best root responding were found at 0.005 g/l IBA. The present optimized protocol enables for any acters who needs large numbers of low land bamboo seedling for industery, small and micro enterprize or for reafforestation programms.

A Concise Review of Dendrocalamus asper and Related Bamboos: Germplasm Conservation, Propagation and Molecular Biology

“…Most researchers have relied on chemicals to control contaminants during plant in vitro propagation, and just a few papers focused on explant disinfection [120,121]. Surface sterilization is a vital step in preparing healthy and viable explants in tissue cultures [122,123]. The type of sterilant use, its concentration and contact time vary for different plant tissue cultures [124].…”