Deuterium Oxide Enhances Escherichia coli SOS Response Induced by Genotoxicants

Абилев, С. К.; Smirnova, S. V.; Igonina, E. V.; Parmon, Valentin N.; Yankovsky, N. K.

doi:10.1134/s0012496618030031

Cited by 6 publications

(4 citation statements)

References 8 publications

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“…In this regard, the importance of water for the implementation of physiological processes is extremely high, but it might vary and differ for each of its nine isotopologues: 1 H 2 16 O, 1 HD 16 O, D 2 16 O, 1 H 2 17 O, 1 HD 17 O, D 2 17 O, 1 H 2 18 O, 1 HD 18 O, D 2 18 O. Some of them, which predominantly contain lighter isotopes, can have in certain concentrations a stimulating effect on functional activity of living systems [5], other isotopologues, including mainly heavy isotopes, are able to inhibit vital processes, especially at high concentrations of deuterium [6,7], and in some cases it was observed that variation of deuterium concentration (both increasing and decreasing its content in water) can enhance the functional activity of living systems [8]. In this regard it is very important to know about the peculiar features of use of certain isotopologues of water for regulation of metabolic processes in a body, and for monitoring of the anabolism and catabolism state of biological substances in various diseases [9].…”

Section: Introductionmentioning

confidence: 99%

Deuterium-Depleted Water Influence on the Isotope 2H/1H Regulation in Body and Individual Adaptation

et al. 2019

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This review article presents data about the influence of deuterium-depleted water (DDW) on biological systems. It is known that the isotope abundances of natural and bottled waters are variable worldwide. That is why different drinking rations lead to changes of stable isotopes content in body water fluxes in human and animal organisms. Also, intracellular water isotope ratios in living systems depends on metabolic activity and food consumption. We found the 2H/1H gradient in human fluids (δ2H saliva >> δ2H blood plasma > δ2Hbreast milk), which decreases significantly during DDW intake. Moreover, DDW induces several important biological effects in organism (antioxidant, metabolic detoxification, anticancer, rejuvenation, behavior, etc.). Changing the isotope 2H/1H gradient from “2H blood plasma > δ2H visceral organs” to “δ2H blood plasma << δ2H visceral organs” via DDW drinking increases individual adaptation by isotopic shock. The other possible mechanisms of long-term adaptation is DDW influence on the growth rate of cells, enzyme activity and cellular energetics (e.g., stimulation of the mitochondrion activity). In addition, DDW reduces the number of single-stranded DNA breaks and modifies the miRNA profile.

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Section: Introductionmentioning

confidence: 99%

Deuterium-Depleted Water Influence on the Isotope 2H/1H Regulation in Body and Individual Adaptation

et al. 2019

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“…Consequently, a disbalance arises between the rate of damage accumulation and the rate of repair of the initial DNA structure by the repair enzymes, leading to the accumulation of damage in the DNA structure. As a result, the SOS response in a cell is activated, providing the repair of the damaged DNA regions [64,65].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Lux Biosensors in Genotoxicological Studies

et al. 2023

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The aim of this study was to assess the applicability of the bacterial lux biosensors for genotoxicological studies. Biosensors are the strains of E. coli MG1655 carrying a recombinant plasmid with the lux operon of the luminescent bacterium P. luminescens fused with the promoters of inducible genes: recA, colD, alkA, soxS, and katG. The genotoxicity of forty-seven chemical compounds was tested on a set of three biosensors pSoxS-lux, pKatG-lux and pColD-lux, which allowed us to estimate the oxidative and DNA-damaging activity of the analyzed drugs. The comparison of the results with the data on the mutagenic activity of these drugs from the Ames test showed a complete coincidence of the results for the 42 substances. First, using lux biosensors, we have described the enhancing effect of the heavy non-radioactive isotope of hydrogen deuterium (D2O) on the genotoxicity of chemical compounds as possible mechanisms of this effect. The study of the modifying effect of 29 antioxidants and radioprotectors on the genotoxic effects of chemical agents showed the applicability of a pair of biosensors pSoxS-lux and pKatG-lux for the primary assessment of the potential antioxidant and radioprotective activity of chemical compounds. Thus, the results obtained showed that lux biosensors can be successfully used to identify potential genotoxicants, radioprotectors, antioxidants, and comutagens among chemical compounds, as well as to study the probable mechanism of genotoxic action of test substance.

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“…№9 дающего действия на ДНК: диоксидин (10 -5 моль/л), индуцирующий образование свободных форм кислорода; фурацилин (2,5•10 -3 моль/л), образующий аддукт с ДНК и подавляющий размножение микроорганизмов; налидиксовая кислота (10 -3 моль/л), подавляющая репликацию ДНК путем ингибирования ДНК-гиразы. Процедура проведения экспериментов описаны в работах [1,3,4]. Эксперимент проводился в двух вариантах.…”

Section: материалы и методыunclassified

“…Во всех случаях статистическая значимость эффектов дейтерирования оказалась исключительно высокой (р < 0,001). Ранее потенцирующий эффект оксида дейтерия также был продемонстрирован в работах со стандартными мутагенами, наиболее часто используемыми в генотоксикологии: 4-нитрохолин-1-оксидом, [3] метилметансульфонатом, N-нитрозо-N-метилмочевиной [4].…”

Section: основные результатыunclassified

Deuterium oxide enhances the SOS response of Escherichia coli induced by bactericidal agents

Smirnova¹,

Шапиро²,

Igonina³

et al. 2020

Nauchno-Prakticheskii Zhurnal «Medicinskaia Genetika»

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Изучали генотоксическое действие бактерицидных средств диоксидина, фурацилина и налидиксовой кислоты на клетки дейтерированной культуры lux-биосенсора E.coli MG1655 (pColD::lux), люминесцирующего в результате активации промотора гена колицина colD в ответ на повреждение ДНК. Впервые показано, что оксид дейтерия (D2O) в концентрации 9% усиливает SOS-ответ, индуцированный исследуемыми лекарственными препаратами, в 1,6-2,8 раза в клетках E. coli. We studied the genotoxic effect of bactericidal agents: dioxine, furaciline and nalidixic acid on cells of the deuterated culture lux-biosensor E. coli MG1655 (pColD::lux), which luminesces as a result of activation of the colicin gene promoter colD in response to DNA damage. For the first time, it was shown that deuterium oxide (D2O) at a concentration of 9% increases the SOS response by 1.6-2.8 times in E. coli cells induced by the studied drugs.

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Deuterium Oxide Enhances Escherichia coli SOS Response Induced by Genotoxicants

Abstract: It has been demonstrated that deuterium oxide enhances the SOS response of Escherichia coli cells induced by chemical genotoxicants and mutagens. This demonstrates that the heavy nonradioactive hydrogen isotope deuterium can be considered to be a comutagen.

Cited by 6 publications

References 8 publications

Deuterium-Depleted Water Influence on the Isotope 2H/1H Regulation in Body and Individual Adaptation

Deuterium-Depleted Water Influence on the Isotope 2H/1H Regulation in Body and Individual Adaptation

Bacterial Lux Biosensors in Genotoxicological Studies

Deuterium oxide enhances the SOS response of Escherichia coli induced by bactericidal agents

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