Deuterium Isotope Effects in Cytology

Flaumenhaft, Eugene; Bose, Shakuntala; Crespi, Henry L.; Katz, Joseph

doi:10.1016/s0074-7696(08)60557-4

Cited by 51 publications

(21 citation statements)

References 132 publications

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“…H 2 O (p‫)99.0ס‬ at 20°C and its high viscosity (p‫)52.1ס‬ compared to H 2 O (p‫)10.1ס‬ at 20°C 9) . The SIE involves the structure of water and macromolecules.…”

Section: Original Articlementioning

confidence: 92%

Effects of Deuterium Oxide on Streptococcus mutans and Pseudomonas aeruginosa

Hirai

Tomida

Kikuchi

et al. 2010

Bull. Tokyo Dent. Coll.

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A complex aggregation of microorganisms growing on a solid substrate is termed a biofilm and is considered to be an etiological agents. Pseudomonas aeruginosa and Streptococcus mutans are representative bacteria in such biofilms. It is well known that deuterium oxide (D 2 O) causes toxic effects on a number of biological systems. We investigated the effects of D 2 O on growth and biofilm formation of P. aeruginosa and S. mutans. These bacteria were incubated in medium containing D 2 O (100%, 75% or 0%) at 37°C for 24hr, 48 hr or 72hr. Growth of P. aeruginosa was inhibited by D 2 O within the first 48hr. However, after 72 hr, growth rate was seen to increase in the D 2 O-containing medium compared with in medium without D 2 O. In contrast, the growth of S. mutans in the D 2 O medium was inhibited within 72hr. The biofilm formation of P. aeruginosa was increased in the D 2 O medium. Biofilm formation of S. mutans in the D 2 O medium increased compared with in the medium without D 2 O, but this increase was only temporary in the case of P. aeruginosa. Compared to biofilm formation in 0% D 2 O medium marked as 100%, the biofilm formation rate of S. mutans in 75% D 2 O medium was 143% at 24hr, 146% at 48 hr and 130% at 72 hr. In other D 2 O concentration media biofilm formation was lower. In 100% D 2 O medium, biofilm formation rate decreased from 114% at 24hr to 56% at 72hr. The biofilm formation rate of P. aeruginosa in 100% D 2 O medium was 172% at 24hr, but decreased to 88% at 72hr. Biofilm formation of P. aeruginosa in 75% and 0% D 2 O media showed no significant difference. We consider that these results were due to stress or alteration in bacterial metabolisms.

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“…H 2 O (p‫)99.0ס‬ at 20°C and its high viscosity (p‫)52.1ס‬ compared to H 2 O (p‫)10.1ס‬ at 20°C 9) . The SIE involves the structure of water and macromolecules.…”

Section: Original Articlementioning

confidence: 92%

Effects of Deuterium Oxide on Streptococcus mutans and Pseudomonas aeruginosa

Hirai

Tomida

Kikuchi

et al. 2010

Bull. Tokyo Dent. Coll.

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“…22,23) Cell Growth in D 2 O Growth of almost all cells was suppressed at high concentrations of D 2 O. [26][27][28][29] Thus, cultivation in D 2 O appears to stress cells. It is known that an increased level of Hsp70 promoted by mild heat treatment makes cells heat-resistant.…”

Section: Isolation and Characteristics Of A Mutant Yeast Cellmentioning

confidence: 99%

Increased Expression of Hsp70 for Resistance to Deuterium Oxide in a Yeast Mutant Cell Line

Unno

Kishido

Morioka

et al. 2003

Biological & Pharmaceutical Bulletin

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Labeling with stable isotopes, typically deuterium (D), is powerful tool for studying the functional structure of biomolecules by NMR. Biosynthesis of certain deuterated proteins in microorganisms cultured in deuterium oxide (D(2)O) is an attractive strategy. However, the growth of almost all microorganisms is inhibited at high concentrations of D(2)O. We isolated a mutant of yeast that grows well in D(2)O. The expression of Hsp70 was enhanced in the mutant. The increased expression also endowed the yeast with cold-resistance. The mutant might be useful for biosynthesis of D-labeled biomolecules.

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“…(51120) BENJAMIN The isotopic substitution of deuterium for hydrogen has been shown to markedly alter the relative rates of chemical reactions and to affect the conformation and stability of macromolecules ( 1 ) . The profound physiological and biological changes associated with high degrees of deuteration have been found not to be compatible with survival in the mammalian organism( 2).…”

Section: Influence Of Deuteration On Circulating Antibody Levels In Thementioning

confidence: 99%

Influence of Deuteration on Circulating Antibody Levels in the Mouse.

Siegel

Morton

1966

Experimental Biology and Medicine

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The isotopic substitution of deuterium for hydrogen has been shown to markedly alter the relative rates of chemical reactions and to affect the conformation and stability of macromolecules ( 1 ) . The profound physiological and biological changes associated with high degrees of deuteration have been found not to be compatible with survival in the mammalian organism( 2). However, the continuous ingestion with 30% deuterium oxide (DzO, heavy water) appears to have no appreciable effects on the body weights, general well-being or life span of the laboratory mouse(3). We have recently demonstrated the protective effects of this, and lower levels of D 2 0 on mice infected with the Rauscher leukemogenic virus (4). The mechanism of protection appeared to be depression of the neoplastic proliferation of lymphoreticular cells. In view of similarities noted between cells undergoing leukemic proliferation and those responding immunologically ( 5) it seemed feasible to investigate the effects of deuteration on the immune response.Matwials and methods. Deuterium oxide (supplied as 99.7% DzO by the Richland Operations Office of the U. S. Atomic Energy Commission, Richland, Wash.) was purified by distillation from alkaline permanganate. BALB/c/jax female mice, 5-7 weeks old, were fed a standard pellet diet and deuterium was administered as drinking water ad libitum at a concentration (v/v) of 30% for the duration of the experiments.Mice received a single intraperitoneal injection of 1.25 mg bovine serum albumin (BSA, crystalline, Pentex, Inc., Kankakee, Ill.) in 0.25 ml of a complete Freund's adjuvant emulsion (Difco Laboratories, Detroit) either 10 days or 17 days after they started drinking heavy water. Sheep erythrocytes were washed 3 times in 20 volumes of cold saline and the packed cells suspended in *Sumpported by U. S. Atomic Energy Commission Contract RLO-192 '1-3. 2 volumes of saline for intraperitoneal injection either as a single dose of 0.25 ml or as two 0.25 ml inoculations 18 hours apart. Mice were bled periodically from the retro-orbital plexus. Antibody titers to BSA were determined by the agglutination of tanned, antigencoated sheep erythrocytes as described by Stavitsky (6). Red cell agglutinins were determined directly in a 2-fold serial dilution system in the presence of 1:200 saline diluted normal rabbit serum. To assay macroglobulin agglutinin activity, aliquots of mouse antisera were incubated for 18 hours at 25°C in phosphate buffered saline, pH 7.4, containing 0.1 M 2-mercaptoethanol(7). Serial dilutions and assays were carried out directly in this medium. Statistical significance of data was determined by student t-test analysis of reciprocal logarithmic (base 2) titer values. Both BSA-adjuvant and sheep erythrocyte immunization experiments were repeated twice using 5-8 mice in each group with similar results as reported below.Results. The antibody response of mice drinking 30% D20 is illustrated in Fig. 1. Mice drinking heavy water continuously starting 10 days in advance of immunization showed no sig...

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Deuterium Isotope Effects in Cytology

Cited by 51 publications

References 132 publications

Effects of Deuterium Oxide on Streptococcus mutans and Pseudomonas aeruginosa

Effects of Deuterium Oxide on Streptococcus mutans and Pseudomonas aeruginosa

Increased Expression of Hsp70 for Resistance to Deuterium Oxide in a Yeast Mutant Cell Line

Influence of Deuteration on Circulating Antibody Levels in the Mouse.

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