Detrusor Quantitative Morphometry in Obstructed Males and Controls

Collado, A.; Batista, Emilio; Gelabert-Más, Antoni; Corominas, Josep M.; Arañó, Pedro; Villavicencio, H.

doi:10.1016/j.juro.2006.07.131

Cited by 36 publications

(46 citation statements)

References 16 publications

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“…The most often used method to evaluate the detrusor collagen content in men is the morphometric analysis of the muscle layer and calculation of the C/M. In the present study BPH patients had a C/M of 0.43, while it varied between 0.27 and 0.56 in other series 9, 10, 11, 16. For the controls the C/M was 0.33 in the present study and varied between 0.14 and 0.25 in other series 9, 10, 11, 16.…”

Section: Discussionmentioning

confidence: 45%

“…Studies have consistently shown increased detrusor collagen content in animal models of obstruction as well as men with BOO/BPH 6, 7, 9, 10, 11, 16. The most often used method to evaluate the detrusor collagen content in men is the morphometric analysis of the muscle layer and calculation of the C/M.…”

Section: Discussionmentioning

confidence: 99%

“…Polarized light was used to intensify the normal birefringence of collagenous fibers. The collagen to smooth muscle ratio (C/M) in the detrusor was obtained by dividing the sum of the collagen fiber area by the sum of the smooth muscle area of the examined fields 9, 10, 11, 16. We investigated the correlation between the C/M and urodynamic parameters.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Increased detrusor collagen is associated with detrusor overactivity and decreased bladder compliance in men with benign prostatic obstruction

Bellucci

Ribeiro

Hemerly

et al. 2017

Prostate International

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BackgroundThis study aimed to investigate the relationship between detrusor collagen content and urodynamic parameters in men with benign prostatic obstruction.Material and methodsNineteen consecutive patients undergoing open prostatectomy for bladder outlet obstruction (BOO) due to benign prostatic hyperplasia (BPH) were evaluated. Urodynamic tests were performed in all patients. BOO and detrusor contractility were assessed with the BOO index (BOOI) and the bladder contractility index (BCI), respectively. A bladder fragment was obtained during prostatectomy. Eight cadaveric organ donors composed the control group. Bladder sections were stained with picrosirius red and hematoxylin-eosin. The collagen to smooth muscle ratio (C/M) in the detrusor was measured and its relationship with urodynamic parameters was investigated.ResultsSeven (36.8%) patients were operated on due to lower urinary tract symptoms and 12 (63.2%) had urinary retention. The mean prostate volume was 128.6 cm3 ± 32.3 cm3, the mean BOOI was 76.4 ± 33.0, and the mean BCI was 116.1 ± 33.7. The mean C/M in BPH patients and controls were 0.43 ± 0.13 and 0.33 ± 0.09, respectively (P = 0.042). A negative correlation was shown between C/M and bladder compliance (r = –0.488, P = 0.043). The C/M was increased in BPH patients with detrusor overactivity (DO) compared to those without DO (0.490 ± 0.110 and 0.360 ± 0.130, respectively; P = 0.030) and also in patients with urinary retention (P = 0.002). No correlation was shown between C/M and maximum cystometric capacity, BOOI, or BCI.ConclusionMen with BOO/BPH have increased detrusor collagen content which is associated with decreased bladder compliance, detrusor overactivity, and urinary retention.

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Section: Discussionmentioning

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Increased detrusor collagen is associated with detrusor overactivity and decreased bladder compliance in men with benign prostatic obstruction

Bellucci

Ribeiro

Hemerly

et al. 2017

Prostate International

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show abstract

“…Optical microscopy studies have demonstrated that a bladder subjected to chronic obstruction that evolves into urinary retention shows significant cellular changes [11], such as an increased detrusor muscle cell diameter and intense intrafascicular collagen deposition. These changes may explain, at least in part, the development of detrusor hypoactivity as a terminal vesical situation [12].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Functional Bladder Recovery in Patients with a Chronic Foley Catheter for Urinary Retention: A Randomized, Prospective, Blind and Comparative Study with 18 Months of Follow-up

Antonio¹,

Furtado²,

Gustavo³

et al. 2017

Int Arch Urol Complic

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Purpose: To analyze the repercussions of chronic Foley catheter use in patients with urinary retention on the functional recovery of the bladder following corrective surgery.Methods: Twenty-four men were randomly selected from the waiting list for prostate surgery in our urology outpatient service database and divided into two groups. One consisted of men with intermediate/severe Lower Urinary Tract Symptoms (LUTS) and other consisted of patients with urinary retention managed with a Foley catheter for a long time, consequent to benign prostatic enlargement. Both groups underwent prostatic surgery. Prior to their operations and at the 6 th , 12 th , and 18 th month follow-ups, they completed the IPSS and ICIQ-SF questionnaires and received a urodynamic evaluation.Results: There were no statistically significant differences in questionnaire scores or in urodynamic parameters at the end of the 18 months of follow-up. Conclusions:Patients experiencing urinary retention, who have been chronic users of a vesical catheter, have a similar postoperative outcome as patients with obstruction but without retention. Preoperative detrusor contraction force seems to be a very important factor in functional vesical recovery outcomes.

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“…In the early stages of BOO, the bladder wall thickens to compensate for higher outlet resistance. However, over time, the detrusor muscle becomes decompensated and then lower urinary tract symptoms (LUTS) appear, ultimately progressing to urinary retention with overflow incontinence [3,4].. Studies have shown that hypertrophy can be found in both smooth muscle cells and the extracellular matrix (ECM) of the bladder wall in BOO [5], where the ECM hypertrophy is mainly due to collagen accumulation, and the more collagen accumulates, the worse the LUTS are [6,7]. The main reason for collagen accumulation in the bladder wall in BOO is inhibited collagen catabolism rather than increased synthesis; this process is controlled by matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) [8,9].…”

Section: Introductionmentioning

confidence: 99%

The Antimuscarinic Agent Tolterodine Regulates Bladder Extracellular Matrix in Partial Bladder Outlet Obstruction in Rats

Yang

Luo

Lin

et al. 2018

Cell Physiol Biochem

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Background/Aims: Antimuscarinic agents can delay the progression of bladder dysfunction caused by bladder outlet obstruction (BOO). To date, the relationship between muscarinic receptor activity and the bladder extracellular matrix (ECM) remains unclear. Thus, an animal model of partial BOO (PBOO) in female rats was established to explore the variation in bladder wall ECM proteins under PBOO conditions with antimuscarinic agent administration. Methods: Rats were randomly divided into three groups: sham, PBOO, and PBOO plus tolterodine. Picrosirius red staining was used to examine the smooth muscle and collagen content of bladder samples. Gene microarray and RT-PCR were performed to survey the expression of ECM proteins, receptors, and metabolism regulators in the rat bladder. Positive results were further evaluated by immunohistochemistry. Results: Picrosirius red staining showed that smooth muscle volume significantly increased in the PBOO and PBOO plus tolterodine groups (p < 0.05), while collagen significantly increased in the PBOO group (p < 0.05) but not in the PBOO plus tolterodine group. Gene microarray and RT-PCR revealed that none of the collagen subtypes exhibited significant changes after PBOO establishment and tolterodine administration. However, matrix metalloproteinases (MMPs) increased significantly in the PBOO plus tolterodine group (p < 0.05). Additionally, PBOO inhibited the expression of noncollagen ECM proteins in the rat bladder wall, while tolterodine induced the expression of noncollagen ECM proteins and ECM receptors. Conclusions: Tolterodine decreased the volume of collagen in PBOO rat bladder wall, possibly via MMPs, and regulated the expression of ECM proteins and receptors.

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Detrusor Quantitative Morphometry in Obstructed Males and Controls

Cited by 36 publications

References 16 publications

Increased detrusor collagen is associated with detrusor overactivity and decreased bladder compliance in men with benign prostatic obstruction

Increased detrusor collagen is associated with detrusor overactivity and decreased bladder compliance in men with benign prostatic obstruction

Evaluation of Functional Bladder Recovery in Patients with a Chronic Foley Catheter for Urinary Retention: A Randomized, Prospective, Blind and Comparative Study with 18 Months of Follow-up

The Antimuscarinic Agent Tolterodine Regulates Bladder Extracellular Matrix in Partial Bladder Outlet Obstruction in Rats

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