Detrital protein contributes to oyster nutrition and growth in the Damariscotta estuary, Maine, USA

Adams, Cheyenne M.; Mayer, Lawrence M.; Rawson, Paul D.; Brady, Damian C.; Newell, Carter R.

doi:10.3354/aei00330

Cited by 13 publications

(13 citation statements)

References 79 publications

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“…As previously described (Adams et al, 2019), algal biomass decreased by a factor of 2.4 during the 12-day incubation. Meanwhile, bacterial abundance increased by a factor of 5.…”

Section: General Characteristics Of the Degradationsupporting

confidence: 74%

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Degradation of Diatom Protein in Seawater: A Peptide-Level View

et al. 2022

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Peptides and proteins were identified during a controlled laboratory degradation of the marine diatom Thalassiosira weissflogii by a surface seawater microbiome. Samples from each time point were processed both with and without the protease trypsin, allowing a partial differentiation between peptides produced naturally by microbial enzymatic degradation and peptides produced from the laboratory digestion of intact protein. Over the 12-day degradation experiment, 31% of the particulate organic carbon was depleted, and there was no preferential degradation of the overall protein pool. However, there was distinct differentiation in the cellular location, secondary structure and modifications between peptides produced by microbial vs. laboratory breakdown. During the initial period of rapid algal decay and bacterial growth, intracellular components from the cytoplasm were consumed first, resulting in the accumulation of membrane-associated proteins and peptides in the detrital pool. Accompanying the enrichment of membrane protein material was an increase in the importance of ɑ-helix motifs. Methylated arginine, a post-translational modification common in cell senescence, was found in high amounts within the microbially produced detrital peptide pool, suggesting a link between in-cell modification and resistance to immediate degradation. Another modification—asparagine deamidation—accumulated within the detrital peptides. Protein taxonomies showed the bacterial community decomposing the algal material was rich in Proteobacteria, and protein annotations showed abundant transportation of solubilized carbohydrates and small peptides across membranes. At this early stage of diagenesis, no changes in bulk amino acids (THAA) were observed, yet a proteomic approach allowed us to observe selective changes in diatom protein preservation by using amino acid sequences to infer subcellular location, secondary structures, and post-translational modifications (PTMs).

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“…As previously described (Adams et al, 2019), algal biomass decreased by a factor of 2.4 during the 12-day incubation. Meanwhile, bacterial abundance increased by a factor of 5.…”

Section: General Characteristics Of the Degradationsupporting

confidence: 74%

“…At each time point (1, 2, 5, and 12-days), 100 mL of the degradation slurry was vacuum filtered onto 25 mm diameter 0.3 um pore size glass fiber membranes (Sterlitech GF75) at 4 • C and stored frozen prior to proteomic extraction and analysis. Further experimental details about the degradation can be found in Adams et al (2019).…”

Section: Algal Degradationmentioning

confidence: 99%

Degradation of Diatom Protein in Seawater: A Peptide-Level View

et al. 2022

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“…It is generally acknowledged that turbidity and associated inorganic matter may limit oyster growth through the dilution of organic dietary constituents (e.g., Thomas et al 2011, Snyder et al 2017, Adams et al 2019, Palmer et al 2020. Furthermore, models of bivalve growth that do not explicitly address responsive adjustments in the filtration and absorption of POM may have limited application between predicted from measures of chlorophyll and SPM (POMPRED, mg L -1 ) and actual POM (POM, mg L -1 ) measured in the same seawater samples from Damariscotta, ME, and Branford, CT, plus 12 other locations in Europe as described by Hawkins et al (2013a).…”

Section: Discussionmentioning

confidence: 99%

“…At the very highest turbidities, dilution of nutritious particles with inorganic sediments which are nondigestible may ultimately limit bivalve growth. For example, Adams et al (2019) found that the American oyster growth rate was a positive function of the CHL/turbidity ratio. Meanwhile, in less turbid estuaries, turbidity can be a useful indication of primary production involving phytoplankton and bioavailable phytodetritus, which provides enzyme hydrolyzable amino acids for bivalve nutrition during algal decay (Adams et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…For example, Adams et al (2019) found that the American oyster growth rate was a positive function of the CHL/turbidity ratio. Meanwhile, in less turbid estuaries, turbidity can be a useful indication of primary production involving phytoplankton and bioavailable phytodetritus, which provides enzyme hydrolyzable amino acids for bivalve nutrition during algal decay (Adams et al 2019). Chlorophyll, an estimate of living phytoplankton biomass, has been related to particulate organic carbon, POC, in the euphotic zone (Legendre & Michaud 1999).…”

Section: Introductionmentioning

confidence: 99%

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