Detrimental effects of polyvinylpyrrolidone on the ultrastructure of spermatozoa (Notulae seminologicae 13)

Strehler, E.; Baccetti, Baccio; Sterzik, K.; Capitani, Serena; Collodel, Giulia; Santo, M. De; Gambera, Laura; Piomboni, Paola

doi:10.1093/humrep/13.1.120

Cited by 78 publications

(59 citation statements)

References 13 publications

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“…Nowadays, viscous media containing synthetic plastic polyvinylpyrrolidone (PVP) are routinely used to reduce sperm motility during ICSI procedure in the majority of AR centers. Nevertheless, some authors maintain that PVP may be toxic for the gametes and the developing embryo [16][17][18]. HA-bound spermatozoa are easily recovered by an injecting pipette; furthermore, HA-containing products have no negative effects on postinjection zygote development and can be metabolised by the oocyte [19][20][21].Thus, the HA-sperm selecting method may represent at least a physiological alternative for slowing sperm motility prior to ICSI.…”

Section: Discussionmentioning

confidence: 99%

Efficiency of hyaluronic acid (HA) sperm selection

Parmegiani

Cognigni

Ciampaglia

et al. 2009

J Assist Reprod Genet

116

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Section: Discussionmentioning

confidence: 99%

Efficiency of hyaluronic acid (HA) sperm selection

Parmegiani

Cognigni

Ciampaglia

et al. 2009

J Assist Reprod Genet

116

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“…Moreover, it has been shown to stabilize the sperm plasma membrane [25], so delaying calcium oscillations in the sperm-penetrated oocyte, as well as preventing nuclear decondensation [26] and DNA lesions [27]. The effect of in vitro treatment by PVP on the ultra structure of human spermatozoa has been tested by statistical analysis [28,29]. PVP had a primary detrimental action on the plasma membrane, as well as on acrosomal and mitochondrial membranes.…”

Section: Discussionmentioning

confidence: 99%

A New Sperm Preparation Method for Testicular Sperm Extraction - Intracytoplasmic Sperm Injection (TESE-ICSI) Cycles: Simple, Effective and Rapid Method

Park

Song

et al. 2002

J.Mamm.Ova.Res.

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Abstract:The aim of t his study was to recover spermatozoa easily from fresh or frozen-thawed testicular sperm extraction (TESE) samples. We simply minced and washed the TESE samples in medium. Spermatozoa were recovered and washed in the bottom of a PVP droplet with an injection pipette and injected into oocytes. In all cycles 100% of spermatozoa (motile and/or immotile) could be recovered. Injected oocytes were fertilized (100% per cycle and in 41.9% of oocytes) and fertilized oocytes were cleaved (100% per cycle; 38.5% of injected oocytes; 91.8% of fertilized oocytes) a f t e r I C S I w i t h f r e s h o r f r o z e n -t h a w e d T E S E spermatozoa. The rates of progression to the embryo stage (2-8 cell) and blastocyst formation and their quality were similar in both the fresh sample group and the frozen-thawed sample group. Both of embryo transfer (73.5%) and embryo-blastocyst transfer (26.5%) were performed successfully. Thirty-four cycles of ICSI with testicular spermatozoa resulted in 23.5% pregnancy rates (fresh sample group, 22.2%; frozen-thawed sample group, 24%) with both embryo transfer (8%) and embryoblastocyst transfer (66.7%). This new sperm preparation method is very simple, easy, effective and rapid for recovering spermatozoa from TESE samples for ICSI. Key words: Fresh or frozen-thawed TESE sample, Spermatozoa recovery, ICSI, Embryo and blastocyst, PregnancyThe introduction of intracytoplasmic sperm injection (ICSI) [1] was originally considered for male factor infertility when spermatozoa could be found in the ejaculate. The possibility of achieving pregnancy with even one available spermatozoon has precipitated the search for a method to obtain spermatozoa from testicular tissue or epididymal sites, when seminal azoospermia is a constant finding. In patients with o b s t r u c t i v e a n d n o n o b st r u c t i v e a z o o s p e r m i a , fertilization and pregnancy can be accomplished by means of a combination of intracytoplasmic sperm injection with epididymal spermatozoa retrieved by microsurgical epididymal sperm aspiration or testicular sperm extraction [2][3][4][5][6][7]. Although ICSI is an effective clinical treatment strategy for male infertility patients, new types of technical difficulties have arisen, because of the extremely small number of spermatozoa handled in this procedure. The recovery of spermatozoa from extremely low quality sperm samples or TESE tissues for use in the ICSI procedure is very difficult [6][7][8].The aim of this study was to recover the spermatozoa easily from fresh or frozen-thawed TESE samples in a 3% polyvinylpyrrolidone (PVP) droplet. Materials and Methods Female patientsWomen under the age of 35 (mean: 28.7; range: 25-35 years) and having many oocytes retrieved (>10) were recruited for this study at

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“…Shorter incubation times in PVP may enhance the quality of injected sperm as incubation in PVP leads to submicroscopic alterations in sperm, such as defects in the acrosomal, mitochondrial, and plasma membranes and deterioration of the chromatin, axonemal tubules, fibrous sheath, and accessory fibers (11,12).…”

Section: Discussionmentioning

confidence: 99%

“…by pellet centrifugation, sperm selected for ICSI must incubate in PVP for longer intervals than what is typically required for epididymal or ejaculated sperm because of the greater difficulty isolating motile testicular sperm from the surrounding extracellular contaminants (3,6). Incubation of sperm in PVP was recently found to cause submicroscopic alterations in sperm (11,12). PVP has a detrimental action on acrosomal, mitochondrial, and plasma membranes of sperm and induces deterioration of the chromatin, axonemal tubules, fibrous sheath, and accessory fibers (11,12).…”

Section: Introductionmentioning

confidence: 99%

“…Incubation of sperm in PVP was recently found to cause submicroscopic alterations in sperm (11,12). PVP has a detrimental action on acrosomal, mitochondrial, and plasma membranes of sperm and induces deterioration of the chromatin, axonemal tubules, fibrous sheath, and accessory fibers (11,12). Prolonged incubation in PVP could cause extensive damage to sperm and lead to decreased fertilization and livebirth rates.…”

Section: Introductionmentioning

confidence: 99%

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Development of a new and efficient laboratory method for processing testicular sperm.

Hammitt¹,

Sattler²,

Ferrigni³

et al. 2001

Fertility and Sterility

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Purpose : Testicular biopsy specimens contain large amounts of debris that makes sperm pickup for ICSI more difficult than with epididymal aspirates. We sought to develop improved processing techniques for testicular sperm extraction (TESE). Methods : Retrievals were with azoospermic male partner scheduled to undergo percutaneous epididymal sperm aspiration (PESA) and TESE. The study group consisted of 9 retrievals with a new TESE technique (TESE-N). The control group was 21 retrievals with PESA and 3 retrievals with a previous TESE technique (TESE-P). Results : TESE-N eliminated almost all debris, which made ICSI sperm pick-up more rapid. TESE-N, PESA, and TESE-P fertilization (77, 75, and 72%) and ongoing/delivered pregnancy rates per retrieval (67, 76, and 67%) were similar. Conclusions : Our new technique provides for easy removal of debris from TESE specimens and fertilization and pregnancy rates equal to epididymal sperm's. Eliminating debris from TESE specimens allows for rapid sperm pick-up for ICSI, making the procedure more efficient for embryology staff.

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Detrimental effects of polyvinylpyrrolidone on the ultrastructure of spermatozoa (Notulae seminologicae 13)

Cited by 78 publications

References 13 publications

Efficiency of hyaluronic acid (HA) sperm selection

Efficiency of hyaluronic acid (HA) sperm selection

A New Sperm Preparation Method for Testicular Sperm Extraction - Intracytoplasmic Sperm Injection (TESE-ICSI) Cycles: Simple, Effective and Rapid Method

Development of a new and efficient laboratory method for processing testicular sperm.

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