Detoxification of high concentrations of trinitrotoluene by bacteria

Solyanikova, Inna P.; Баскунов, Б. П.; Baboshin, M. A.; Saralov, A. I.; Golovleva, L. A.

doi:10.1134/s0003683812010152

Cited by 27 publications

(11 citation statements)

References 15 publications

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“…Recent reports on explosive degradation indicate the usefulness of mixed bacteria in soil and nutrient conditions for maximum removal of explosives (Markis et al 2010;Ziganshin et al 2010;Montgomery et al 2011;Rylott et al 2011;Mutter et al 2012;Solyanikova et al 2012;Claus, 2014). Another aspect of research involves hydride-mediated reduction of TNT with subsequent degradation by yeast.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of 2,4,6-trinitrotoluene (TNT) under sulfate and nitrate reducing conditions

Boopathy

2014

Biologia

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Anaerobic degradation of 2,4,6-trinitrotoluene (TNT) was studied under sulfate-and nitrate-reducing conditions using enrichment cultures developed from a TNT-contaminated soil from the Louisiana Army Ammunition Plant (LAAP) in Minden, Louisiana, USA. The soil samples were enriched using mineral salt media with either nitrate or sulfate as electron acceptors in the presence of TNT under strict anaerobic conditions. The enriched samples were experimented with TNT as either the sole source of carbon or nitrogen and also under co-metabolic conditions with molasses as co-substrate. The results revealed that TNT was removed under both electron acceptor conditions. However, the TNT degradation efficiency was significantly higher under sulfate-reducing conditions than the nitrate-reducing conditions. Under sulfatereducing conditions, TNT removal was faster when molasses was used as co-substrate. The metabolic analysis showed that TNT was mineralized and the major end product was acetic acid, CO2, and ammonia. A soil slurry reactor with TNT-contaminated soil showed more than 90% of TNT removal within 60 days of incubation.

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Section: Introductionmentioning

confidence: 99%

Biodegradation of 2,4,6-trinitrotoluene (TNT) under sulfate and nitrate reducing conditions

Boopathy

2014

Biologia

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“…The experimental data showed that the number of local (indigenous) microorganisms reduced after 40 and 100 days; it was caused by the fact that part of local microorganisms could not undergo adaptation to the introduced toxicant. While at the first stage(introducing selected strains), the amount of microorganisms existing in the soil slightly decreased, but later, returned again to the initial amount or in some cases, even insignificantly exceeded it (Table 6 and Fig. 6).…”

Section: Volume 2 Issue 1 | January 2018mentioning

confidence: 98%

“…Based on the 20 years experience of the authors, freshly isolated and collection strains representing the following genera Aspergillus, Trichoderma and Mucor were recognized by ability for TNT degradation. Additionally ,according to the data of recent years, some strains of these genera have feature of TNT assimilation (6). At first, screening was conducted on Czapek's solid modified medium #1 with different concentrations of TNT (100 mg/l; 300 mg/l, 400 mg/l) as a sole source of carbon (these concentrations exceed TNT permissible concentration).…”

Section: Selection Of Strains Actively Degrading Tntmentioning

confidence: 99%

“…Nowadays, strains-destructors of organic toxicants mainly belonging to bacteria and basidial fungi (white-rot fungi) have been studied (5,6). In comparison with other taxonomic groups of microorganisms, the detoxification potential of microscopic fungi is investigated in a less extent; however, according to the data of last decade, representatives of some genera of microscopic fungi, in particular, zygo-and deuteromycetes also displayed an ability to decompose 2,4,6-TNT and other toxic compounds and mineralize them (7).…”

Section: Introductionmentioning

confidence: 99%

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Fungal elimination of 2,4,6-trinitrotoluene (TNT) from the soils

Kutateladze

Zakariashvili

Khokhashvili

et al. 2018

The EuroBiotech Journal

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The analysis of microscopic fungi collection created at theDurmishidze Institute of Biochemistry and Biotechnology revealed 107 strains assimilating 2,4,6-TNT (2,4,6-trinitrotoluene) belonging to the different fungal genera. The strains have been isolated from the polluted areas adjacent to the military grounds and industrial waste waters. It has been shown TNT is degraded most actively by strains belonging to the following genera: Trichoderma, Aspergillus, Mucor and Trichoderma. Optimal cultivation conditions for highly active strains -the destructors of TNT have been revealed.It has been established that the carbon skeleton of TNT being utilized by the mentioned strains undergoes biotransformation. The existence of radioactive intermediates of biotransformation, organic acids (70-90%) and amino acids (10-30%) have been detected in liquid culture. Radioactive label of 1-14 C-TNT is mostly found in fumaric acid, which is known as one of the main products of benzene biotransformation and further conversion into succinic acid.Remediation level of TNT-contaminated red and black soils treated by the most active strains Aspergillus nigerN2-2 and Mucor sp. T1-1 have been studied under laboratory and field conditions. Cultivation of the above mentioned strains under laboratory conditions in sterile, black and red soils for 30 days at 30°C allowed decreasing the content of TNT in black soil to the residual, and in red soil -to 15%; cultivation of Aspergillus niger N2-2 decreased the amount of TNT in black soil to 11 and in red soilto 21%. Under field conditions, TNT degradation level in contaminated soils by naturally existing micro flora during 100 days was equal to 40-50%, and in the case of additional introduction of both fungal strains, TNT-destructors reached 80%.

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“…Several by-products and metabolites such as 2,4-DNT, 2,6-DNT, 2-A-2,6-DNT, and 4-A-2,4-DNT were formed during this process. Other studies demonstrated that the complete TNT degradation by aerobic bacteria resulted in accumulation of ADNTs as key metabolites (Solyanikova et al 2012). Recent studies found that Achromobacter spanius STE 11 Vorbeck et al (1994) Pseudomonas aeruginosa Nitrite releasing from TNT and transformed to 2A- 4NT Kalafut et al (1998) Pseudomonas fluorescens NADPH flavoprotein oxidoreductase catalyzing multiple transformations, generating ArNHOH, H-TNT and H 2 -TNT Pak et al (2000) Pseudomonas pseudoalcaligenes…”

Section: Tnt Biodegradation Routes and By-product Analysesmentioning

confidence: 99%

Bioremediation of 2,4,6-trinitrotoluene-contaminated groundwater using unique bacterial strains: microcosm and mechanism studies

Sheu

Lien

Chen

et al. 2016

Int. J. Environ. Sci. Technol.

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Groundwater at many military factory, munition storage and maneuver sites is contaminated by explosives chemicals that were released into the subsurface. The 2,4,6-trinitrotoluene (TNT) is among the most common explosive pollutants. In this study, two TNT-degrading strains, isolated from TNT-contaminated soils and wastewater sludge, were applied for TNT biodegradation. Based on the 16S rDNA sequence analyses, these two bacterial strains were identified as Achromobacter sp. and Klebsiella sp. via biochemical and DNA analyses. Microcosm study was conducted to evaluate the feasibility and efficiency of TNT biodegradation under aerobic conditions. Results indicate that TNT degradation by-products were detected in microcosms (inoculated with Achromobacter sp. and Klebsiella sp.) with cane molasses addition. Klebsiella sp. and Achromobacter sp. used TNT as the nitrogen source and caused completely removal of TNT. Two possible TNT biodegradation routes could be derived: (1) part of the TNT was transformed to nitrotoluene then transformed to nitrobenzene followed by the nitro substitute process, and trinitrobenzene, dinitrobenzene, and nitrobenzene were detected; and (2) TNT was transformed via the nitro substitute mechanism, and dinitrotoluene followed by nitrotoluene isomers were detected. The initial TNT degradation involved the reduction or removal of the nitro substitute to an amino derivative or free nitrite. Results show that the second route was the dominant TNT biodegradation pathway. The produced by-products were also degraded without significant accumulation during the degradation process. These findings would be helpful in designing a practical system inoculated with isolated TNT degradation strains for the treatment of TNT-contained groundwater.

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Detoxification of high concentrations of trinitrotoluene by bacteria

Cited by 27 publications

References 15 publications

Biodegradation of 2,4,6-trinitrotoluene (TNT) under sulfate and nitrate reducing conditions

Biodegradation of 2,4,6-trinitrotoluene (TNT) under sulfate and nitrate reducing conditions

Fungal elimination of 2,4,6-trinitrotoluene (TNT) from the soils

Bioremediation of 2,4,6-trinitrotoluene-contaminated groundwater using unique bacterial strains: microcosm and mechanism studies

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