Detoxification of a Lignocellulosic Waste from a Pulp Mill to Enhance Its Fermentation Prospects

Llano, Tamara; Quijorna, Natalia; Coz, Alberto

doi:10.3390/en10030348

Cited by 27 publications

(17 citation statements)

References 48 publications

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“…In our previous study [13], neutralized beech wood diluted acid hydrolysate at a 12% loading partially inhibited Chlorella growth, when compared to a synthetic organic carbon-based medium. In the literature, lignocellulosic hydrolysates contain a range of compounds that inhibit the metabolism of yeast [26,27], and can also negatively affect microalgae growth [10]. In the present study, neutralized enzymatic beech wood hydrolysate at 10% loading (TGP-Enz10) also inhibited Chlorella growth.…”

Section: Effect Of Enzymatic Hydrolysate On Chlorella Biomass Fatty mentioning

confidence: 49%

Effect of Enzymatic Beech Fagus Sylvatica Wood Hydrolysate on Chlorella Biomass, Fatty Acid and Pigment Production

et al. 2017

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This work evaluates the possibility of applying enzymatic beech wood (Fagus sylvatica) hydrolysate as a feedstock for Chlorella sorokiniana growth, and fatty acid and pigment production. Beech wood solids were pretreated with NaOH at high temperature to partially remove xylose and Klason lignin, and enable production of glucose during subsequent enzymatic hydrolysis. Neutralized wood enzymatic hydrolysate containing glucose (TGP-Enz10), was tested on Chlorella growth during heterotrophic cultivation and compared with microalgae growth in a medium containing synthetic glucose (TGP). Results show that enzymatic hydrolysate enabled Chlorella growth in the dark for biomass, fatty acid and pigment production due to the presence of glucose, although the productivity obtained was smaller, if compared to heterotrophic cultivation in a synthetic TGP medium. Partial growth inhibition and diminished productivity in wood hydrolysate supplemented Chlorella culture was due to the presence of neutralized citrate buffer. Neutralized citrate buffer (TGP-Cit10) was found to partially inhibit heterotrophic growth and also strongly suppress mixotrophic growth in Chlorella culture. This buffer was also shown to alter fatty acid composition and to slightly affect Chl Total /Car Total ratio during heterotrophic cultivation. Heterotrophic Chlorella cultivation with TGP-Enz10 showed that wood enzymatic hydrolysate can constitute a potential feedstock for microalgae cultivation, although the composition of the buffer used during enzymatic hydrolysis should be taken into consideration.

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Section: Effect Of Enzymatic Hydrolysate On Chlorella Biomass Fatty mentioning

confidence: 49%

Effect of Enzymatic Beech Fagus Sylvatica Wood Hydrolysate on Chlorella Biomass, Fatty Acid and Pigment Production

et al. 2017

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“…In contrast, the findings of Yadav et al ., Prasertwasu et al . and Llano et al . elucidated maximum removal of phenolic compounds by overliming of rice straw, Thai mission grass and pulp mill hydrolysates, respectively at pH 10.…”

Section: Resultsmentioning

confidence: 99%

Removal of phenolic compounds through overliming for enhanced saccharification of wheat straw

Haq

Arshad

Nawaz

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND The deactivation of cellulases by phenolics is considered to be the main bottleneck in the saccharification of agricultural by‐products for efficient bioethanol production on a large scale. The removal of these phenolic inhibitors, produced as a result of pretreatment, is essential for an efficient saccharification process. One such process is overliming that has been mostly studied for hydrolysates of agricultural by‐products. In the present study however, solid biomass was used whereby the detection of phenolic compounds and their effect on saccharification was studied. RESULTS The pretreated wheat straw showed 19% saccharification and total phenolic content (TPC) of 188 mg gallic acid equivalent g‐1 biomass dry weight. Detoxification of pretreated wheat straw applying different alkalis, i.e. NaOH, NH4OH and Ca(OH)2 was conducted. Overliming using Ca(OH)2 solution of pH 12, for 2 h at 80°C showed significant removal of 60% of phenolic compounds, which in turn enhanced the saccharification by 2.4‐fold. Scanning electron microscopy of pretreated and overlimed substrates also showed considerable change in structure, making it more accessible for enzymatic hydrolysis. CONCLUSION The elimination of phenolic compounds due to overliming has facilitated increased saccharification that in turn will help in substrate efficient biofuel production. © 2018 Society of Chemical Industry

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“…These inhibitors can be removed by applying the proper detoxification process [36]. Several detoxification methods such as neutralization, overliming with calcium hydroxide, adsorption with activated carbon, ion exchange resins, laccase enzyme [37][38][39][40] are known for removing various inhibitory compounds from lignocellulosic hydrolysates.…”

Section: Introductionmentioning

confidence: 99%

Utility of starchy, lignocellulosics and cellulosics hydrolysates on cellulase production under liquid state fermentation

Verma¹,

Kumar²,

Bansal³

2019

Waste Dispos. Sustain. Energy

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Starch is one of the most abundant renewable carbohydrate reserves of higher plants. It can be used to produce many valuable food products in the food processing industry. Furthermore, starch is also used as an important feedstock in the fermentation industry to produce value-added products. Lignocellulosic materials such as agriculture and forestry wastes are considered as a renewable feedstock for bioenergy production through a biochemical conversion process. Converting lignocellulosic biomass into fuels and chemicals entail a physicochemical pretreatment of the biomass, followed by enzymatic hydrolysis of the polysaccharide components such as cellulose and hemicellulose into monomeric sugars. These sugars can then be further fermented into other desired compounds of interest. During the deconstruction processes, various inhibitory compounds are released due to the partial over-degradation of lignocellulose biomass, which inhibits the cell growth and metabolic capacity of fermenting strains. Cellulosic materials such as waste paper in large quantities can also be used as potentially cheap feedstock for sustainable production of value-added products. The present investigation is mainly focused on the utility of starchy hydrolysates (wheat, potato, and rice) and lignocellulosics hydrolysates (bagasse and wheat straw) in cellulase production under liquid state fermentation. It also depicts the potential of cellulosic hydrolysate (waste newspaper) in product formation.

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Detoxification of a Lignocellulosic Waste from a Pulp Mill to Enhance Its Fermentation Prospects

Cited by 27 publications

References 48 publications

Effect of Enzymatic Beech Fagus Sylvatica Wood Hydrolysate on Chlorella Biomass, Fatty Acid and Pigment Production

Effect of Enzymatic Beech Fagus Sylvatica Wood Hydrolysate on Chlorella Biomass, Fatty Acid and Pigment Production

Removal of phenolic compounds through overliming for enhanced saccharification of wheat straw

Utility of starchy, lignocellulosics and cellulosics hydrolysates on cellulase production under liquid state fermentation

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