Detoxification of 1,1,2-trichloroethane to ethene in a bioreactor co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains

Mortan, Siti Hatijah; Martín-González, Lucía; Vicent, Teresa; Caminal, Glòria; Nijenhuis, Ivonne; Adrian, Lorenz; Marco‐Urrea, Ernest

doi:10.1016/j.jhazmat.2017.02.043

Cited by 22 publications

(13 citation statements)

References 26 publications

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“…Chlorinated compounds were quantified by analysing 500 µL of headspace samples by gas chromatography (GC) coupled to a flame ionization detector (FID), as described elsewhere (Martín-González et al, 2015). Lactate, acetate and other short-chain fatty acids (VFAs) were monitored by HPLC from 1 mL filtered liquid samples (0.22 μm, Millex), as previously reported (Mortan et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Use of dual element isotope analysis and microcosm studies to determine the origin and potential anaerobic biodegradation of dichloromethane in two multi-contaminated aquifers

Blázquez-Pallí

Shouakar-Stash

Palau

et al. 2019

Science of The Total Environment

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Many aquifers around the world are impacted by toxic chlorinated methanes derived from industrial processes due to accidental spills. Frequently, these contaminants co-occur with chlorinated ethenes and/or chlorinated benzenes in groundwater, forming complex mixtures that become very difficult to remediate. In this study, a multi-method approach was used to provide lines of evidence of natural attenuation processes and potential setbacks in the implementation of bioremediation strategies in multi-contaminated aquifers. First, this study determined i) the carbon and chlorine isotopic compositions (δ 13 C, δ 37 Cl) of several commercial pure phase chlorinated compounds, and ii) the chlorine isotopic fractionation (εCl =-5.2 ± 0.6‰) and the dual C-Cl isotope correlation (Λ C/Cl = 5.9 ± 0.3) during dichloromethane (DCM) degradation by a Dehalobacteriumcontaining culture. Such data provide valuable information for practitioners to support the interpretation of stable isotope analyses derived from polluted sites. Second, the bioremediation potential of two industrial sites contaminated with a mixture of organic pollutants (mainly DCM, chloroform (CF), trichloroethene (TCE), and monochlorobenzene (MCB)) was evaluated. Hydrochemistry, dual (C-Cl) isotope analyses, laboratory microcosms, and microbiological data were used to investigate the origin, fate and biodegradation potential of chlorinated methanes. At Site 1, δ 13 C and δ 37 Cl compositions from field samples were consistent with laboratory microcosms, which showed complete degradation of CF, DCM and TCE, while MCB remained.

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Section: Methodsmentioning

confidence: 99%

Use of dual element isotope analysis and microcosm studies to determine the origin and potential anaerobic biodegradation of dichloromethane in two multi-contaminated aquifers

Blázquez-Pallí

Shouakar-Stash

Palau

et al. 2019

Science of The Total Environment

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“…CEs concentrations were analysed from 500 µL headspace samples by gas chromatography (GC) coupled to a flame ionization detector (FID) as reported by Martín-González et al (2015). VFAs (lactate, pyruvate, acetate, formate) were analysed by high performance liquid chromatography (HPLC) from filtered liquid samples as described elsewhere (Mortan et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Integrative isotopic and molecular approach for the diagnosis and implementation of an efficient in-situ enhanced biological reductive dechlorination of chlorinated ethenes

Blázquez-Pallí

Rosell

Varias³

et al. 2019

Water Research

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Based on the previously observed intrinsic bioremediation potential of a site originally contaminated with perchloroethene (PCE), field-derived lactate-amended microcosms were performed to test different lactate isomers and concentrations, and find clearer isotopic and molecular parameters proving the feasibility of an in-situ enhanced reductive dechlorination (ERD) from PCE-to-ethene (ETH). According to these laboratory results, which confirmed the presence of Dehalococcoides sp. and the vcrA gene, an in-situ ERD pilot test consisting of a single injection of lactate in a monitoring well was performed and monitored for 190 days. The parameters used to follow the performance of the ERD comprised the analysis of i) hydrochemistry, including redox potential (Eh), and the concentrations of redox sensitive species, chlorinated ethenes (CEs), lactate, and acetate; ii) stable isotope composition of carbon of CEs, and sulphur and oxygen of sulphate; and iii) 16S rRNA gene sequencing from groundwater samples. Thus, it was proved that the injection of lactate promoted sulphate-reducing conditions, with the subsequent decrease in Eh, which allowed for the full reductive dechlorination of PCE to ETH in the injection well. The biodegradation of CEs was also confirmed by the enrichment in 13 C and carbon isotopic mass balances. The metagenomic results evidenced the shift in the composition of the microbial population towards the predominance of fermentative bacteria. Given the success of the in-situ pilot test, a full-scale ERD with lactate was then implemented at the site. After one year of treatment, PCE and trichloroethene were mostly depleted, whereas vinyl chloride (VC) and ETH were the predominant metabolites. Most importantly, the shift of the carbon isotopic mass balances towards more positive values confirmed the complete reductive dechlorination, including the VC-to-ETH reaction step. The combination of techniques used here provides complementary lines of evidence for the diagnosis of the intrinsic biodegradation potential of a polluted site, but also to monitor 3 the progress, identify potential difficulties, and evaluate the success of ERD at the field scale.

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“…For all samples, concentrations of target contaminants were quantified by analysing bottles headspace as indicated elsewhere [15]. Lactate and other short fatty-acids were monitored by HPLC [16].…”

Section: Methodsmentioning

confidence: 99%

Use of C–Cl CSIA to elucidate origin and fate of DCM in complex contaminated field sites

et al. 2019

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We used C-Cl dual isotope analysis and microcosm studies for elucidating the origin and fate of the common groundwater pollutant dichloromethane (DCM) in two different multi-contaminant field sites in Catalonia, Spain; where DCM contamination could be the result of direct solvent releases and/or chloroform (CF) transformation. Known commercial solvents isotopic compositions as well as characteristic C-Cl dual isotope slopes from our anaerobic enrichment culture containing Dehalobacterium sp., capable of fermenting DCM, and other bacteria from the literature were used for field data interpretation.

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Detoxification of 1,1,2-trichloroethane to ethene in a bioreactor co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains

Cited by 22 publications

References 26 publications

Use of dual element isotope analysis and microcosm studies to determine the origin and potential anaerobic biodegradation of dichloromethane in two multi-contaminated aquifers

Use of dual element isotope analysis and microcosm studies to determine the origin and potential anaerobic biodegradation of dichloromethane in two multi-contaminated aquifers

Integrative isotopic and molecular approach for the diagnosis and implementation of an efficient in-situ enhanced biological reductive dechlorination of chlorinated ethenes

Use of C–Cl CSIA to elucidate origin and fate of DCM in complex contaminated field sites

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