2023
DOI: 10.1647/21-00075
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Determining the Prevalence of Avian Chlamydiosis in Wild Amazona Species From Brazil Using Molecular Testing and Clinical Signs

Bernardo Mirabal Santos,
Edma Santos de Antonio,
Douglas Campos Pereira
et al.
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“…The conventional extraction methods demand higher skills from the researcher in the standardization of protocols, and this might be the reason why they are less commonly used now, taking into account the number of commercial kits available. However, for wild animal screening centers with large groups of birds where there is a need for routine evaluations of several pathogens, it is worthwhile to consider the use of conventional methods for the extraction of nucleic acids in order to reduce the final costs of the polymerase chain reaction (PCR) without interfering in the quality of the genetic material extracted [ 46 , 47 ]. Cost reduction is especially important in countries with a high number of animals received by wild animal screening centers and with low investment in the research area, such as Brazil and Indonesia.…”
Section: Differential Aspects Of Viral Diagnosis In Psittacine Birdsmentioning
confidence: 99%
“…The conventional extraction methods demand higher skills from the researcher in the standardization of protocols, and this might be the reason why they are less commonly used now, taking into account the number of commercial kits available. However, for wild animal screening centers with large groups of birds where there is a need for routine evaluations of several pathogens, it is worthwhile to consider the use of conventional methods for the extraction of nucleic acids in order to reduce the final costs of the polymerase chain reaction (PCR) without interfering in the quality of the genetic material extracted [ 46 , 47 ]. Cost reduction is especially important in countries with a high number of animals received by wild animal screening centers and with low investment in the research area, such as Brazil and Indonesia.…”
Section: Differential Aspects Of Viral Diagnosis In Psittacine Birdsmentioning
confidence: 99%