Determining the period, phase and anticipatory component of activity and temperature patterns in newborn rabbits that were maintained under a daily nursing schedule and fasting conditions

Trejo-Muñoz, Lucero; Navarrete, Erika; Montúfar-Chaveznava, Rodrigo; Caldelas, Ivette

doi:10.1016/j.physbeh.2012.04.005

Cited by 9 publications

(21 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the first segment (P2 to P4), in a previous study conducted by our group, we found that the 24-h rhythm was not fully consolidated [13]. In the second segment (P5 to P7), which was considered the pre-visual stage, we found that the 24-h rhythmicity was evident [13]. …”

Section: Methodsmentioning

confidence: 75%

“…The individual rabbits’ locomotor activity and body temperature were recorded simultaneously using telemetry according to previously described methods [13]. On P1, the rabbit pups were anesthetized by the inhalation of Sevoflurane (Sevorane, Abbot, USA) and were implanted i.p.…”

Section: Methodsmentioning

confidence: 99%

“…One remarkable example is the European newborn rabbit ( Oryctolagus cuniculus ). This species exhibits 24-h rhythmicity in a range of parameters, at the behavioral [11–13],, physiological [13–16] and molecular levels [16–18]. Maternal care is restricted in rabbits because the mother abandons the nest as soon as she gives birth to the pups and only visits them once every 24 h and nurses for 3 to 5 min [11,19,20].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, newborn rabbits anticipate the regular arrival of the lactating female with an increase in general arousal [11–13], body temperature [13,15] and plasma corticosterone levels [21]. This anticipation of nursing persists even if the rabbits are isolated from the mother, indicating that these changes are controlled by the circadian system [13,15].…”

Section: Introductionmentioning

confidence: 99%

“…This anticipation of nursing persists even if the rabbits are isolated from the mother, indicating that these changes are controlled by the circadian system [13,15]. Interestingly, this anticipatory phenomenon during development has only been reported in rabbits [11–13,15,16,22,23]. It has been widely described in rodents but only during adult life (reviewed in [24,25]).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Maternal Olfactory Cues Synchronize the Circadian System of Artificially Raised Newborn Rabbits

Montúfar-Chaveznava¹,

Trejo-Muñoz

Hernández-Campos

et al. 2013

PLoS ONE

Self Cite

View full text Add to dashboard Cite

In European newborn rabbits, once-daily nursing acts as a strong non-photic entraining cue for the pre-visual circadian system. Nevertheless, there is a lack of information regarding which of the non-photic cues are capable of modulating pup circadian system. In this study, for the first time, we determined that the mammary pheromone 2-methylbut-2-enal (2MB2) presented in the maternal milk acts as a non-photic entraining cue. We evaluated the effect of once-daily exposure to maternal olfactory cues on the temporal pattern of core body temperature, gross locomotor activity and metabolic variables (liver weight, serum glucose, triacylglycerides, free fatty acids, cholecystokinin and cholesterol levels) in newborn rabbits. Rabbit pups were separated from their mothers from postnatal day 1 (P1) to P8 and were randomly assigned to one of the following conditions: nursed by a lactating doe (NAT); exposed to a 3-min pulse of maternal milk (M-Milk), mammary pheromone (2MB2), or water (H2O). To eliminate maternal stimulation, the pups of the last three groups were artificially fed once every 24-h. On P8, the rabbits were sacrificed at different times of the day. In temperature and activity, the NAT, M-Milk and 2MB2 groups exhibited clear diurnal rhythmicity with a conspicuous anticipatory rise hours prior to nursing. In contrast, the H2O group exhibited atypical rhythmicity in both parameters, lacking the anticipatory component. At the metabolic level, all of the groups exhibited a diurnal pattern with similar phases in liver weight and metabolites examined. The results obtained in this study suggest that during pre-visual stages of development, the circadian system of newborn rabbits is sensitive to the maternal olfactory cues contained in milk, indicating that these cues function as non-photic entraining signals mainly for the central oscillators regulating the expression of temperature and behavior, whereas in metabolic diurnal rhythmicity, these cues lack an effect, indicating that peripheral oscillators respond to milk administration.

show abstract

Section: Methodsmentioning

confidence: 75%

Section: Methodsmentioning

confidence: 99%