Determining the optimum cut-off IgM/ IgG ratio for predicting secondary dengue infections: An observational hospital based study from Central India

Agarwal, Ankita; Jain, Rajeev; Chaurasia, Deepti; Biswas, Debasis

doi:10.1016/j.ijmmb.2022.08.015

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…Our data showed based on Chi Square analysis, that no correlation occurred between the day of fever and to IgM antibody (p>0.05). Another study held by Agarwal et al, demonstrated IgM, combined with IgG dengue (IgM/IgG ratio), as a laboratory parameter to predict severe infection and dengue shock syndrome, mainly in a secondary infection (Agarwal et al, 2022;Satriadi et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

The Relationship of Fever Onset with NS1 Examination Results, IgM, and IgG in Dengue Hemorrhagic Fever Patients

Merizka,

Alifah,

Handayani

et al. 2023

J.Ilm.Kedokt. Wijaya Kusuma

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Fever onset represents the average number of days from the beginning of fever symptoms to the time when IgM and IgG examinations are conducted. Determining the right timing for these examinations can significantly impact their accuracy. In November 2021, there was a notable surge in cases of Dengue Hemorrhagic Fever at the Karawang Health Center, Sukabumi. This study aimed to investigate the relationship between the duration of fever and the results of dengue antigen and antibody tests. The research employed a descriptive analysis with a cross-sectional approach, and a sample of 50 individuals was gathered through consecutive sampling. The data was then analyzed using the Chi-Square test. The study revealed a positive correlation between the duration of fever and the outcomes of the NS1 and IgG Dengue examinations (p<0.05), highlighting the significance of timing when conducting these tests. NS1 was predominantly detected within the first three days of fever, while IgG showed reactivity after more than five days of fever. These findings underscore the importance of timing when conducting these tests and interpreting their results.

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Section: Discussionmentioning

confidence: 99%

The Relationship of Fever Onset with NS1 Examination Results, IgM, and IgG in Dengue Hemorrhagic Fever Patients

Merizka,

Alifah,

Handayani

et al. 2023

J.Ilm.Kedokt. Wijaya Kusuma

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“…When the result is less than 1:1280, a primary infection is usually assumed 42 . A study employed IgG ELISA and HI to investigate whether it could discriminate among acute and secondary infections 43 . The sample OD was divided by the use of the calibrator OD in the IgG ELISA.…”

Section: Conventional Methodsmentioning

confidence: 99%

“…42 A study employed IgG ELISA and HI to investigate whether it could discriminate among acute and secondary infections. 43 The sample OD was divided by the use of the calibrator OD in the IgG ELISA. Primary infection was defined as any value below 1, whereas secondary infection was defined as any value over 1.…”

Section: Hemagglutination Inhibition (Hi) Testsmentioning

confidence: 99%

Innovations in dengue virus detection: An overview of conventional and electrochemical biosensor approaches

Raza,

Poria,

Kala

et al. 2024

Biotech and App Biochem

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Globally, people are in great threat due to the highly spreading of viral infectious diseases. Every year like 100–300 million cases of infections are found, and among them, above 80% are not recognized and irrelevant. Dengue virus (DENV) is an arbovirus infection that currently infects people most frequently. DENV encompasses four viral serotypes, and they each express comparable sign. From a mild febrile sickness to a potentially fatal dengue hemorrhagic fever, dengue can induce a variety of symptoms. Presently, the globe is being challenged by the untimely identification of dengue infection. Therefore, this review summarizes advances in the detection of dengue from conventional methods (nucleic acid‐based, polymerase chain reaction‐based, and serological approaches) to novel biosensors. This work illustrates an extensive study of the current designs and fabrication approaches involved in the formation of electrochemical biosensors for untimely identifications of dengue. Additionally, in electrochemical sensing of DENV, we skimmed through significances of biorecognition molecules like lectins, nucleic acid, and antibodies. The introduction of emerging techniques such as the CRISPR/Cas’ system and their integration with biosensing platforms has also been summarized. Furthermore, the review revealed the importance of electrochemical approach compared with traditional diagnostic methods.

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“…In acute samples single IgM/IgG ratio of>=1.2 was consistent with primary DENV infection and <1.2 with secondary DENV infection, with >90 % agreement with the prior method in use (HI) in one study [171] and with a composite serologic standard in another [177]. Other studies have found different cut-offs, varying between 1.20 and 2 [176, 178]. A study using PRNT predominant serotype at 6 months as compared with NAAT-confirmed serotype at time of infection to define primary and secondary DENV infection found that the optimal cut-off declined with time after infection but was broadly consistent with the cut-offs previously quoted [179].…”

Section: Antibody Detectionmentioning

confidence: 99%

Dengue: A review of laboratory diagnostics in the vaccine age

Frazer,

Norton

2024

Journal of Medical Microbiology

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Background. Dengue is an important arboviral infection of considerable public health significance. It occurs in a wide global belt within a variety of tropical regions. The timely laboratory diagnosis of Dengue infection is critical to inform both clinical management and an appropriate public health response. Vaccination against Dengue virus is being introduced in some areas. Discussion. Appropriate diagnostic strategies will vary between laboratories depending on the available resources and skills. Diagnostic methods available include viral culture, the serological detection of Dengue-specific antibodies in using enzyme immunoassays (EIAs), microsphere immunoassays, haemagglutination inhibition or in lateral flow point of care tests. The results of antibody tests may be influenced by prior vaccination and exposure to other flaviviruses. The detection of non-structural protein 1 in serum (NS1) has improved the early diagnosis of Dengue and is available in point-of-care assays in addition to EIAs. Direct detection of viral RNA from blood by PCR is more sensitive than NS1 antigen detection but requires molecular skills and resources. An increasing variety of isothermal nucleic acid detection methods are in development. Timing of specimen collection and choice of test is critical to optimize diagnostic accuracy. Metagenomics and the direct detection by sequencing of viral RNA from blood offers the ability to rapidly type isolates for epidemiologic purposes. Conclusion. The impact of vaccination on immune response must be recognized as it will impact test interpretation and diagnostic algorithms.

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Determining the optimum cut-off IgM/ IgG ratio for predicting secondary dengue infections: An observational hospital based study from Central India

Cited by 4 publications

References 23 publications

The Relationship of Fever Onset with NS1 Examination Results, IgM, and IgG in Dengue Hemorrhagic Fever Patients

The Relationship of Fever Onset with NS1 Examination Results, IgM, and IgG in Dengue Hemorrhagic Fever Patients

Innovations in dengue virus detection: An overview of conventional and electrochemical biosensor approaches

Dengue: A review of laboratory diagnostics in the vaccine age

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