Determining the influence of N-acetylation on water sorption in chitosan films

Gámiz-González, M.A.; Piskin, A.E.; Pandis, C.; Chatzimanolis-Moustakas, C.; Kyritsis, Apostolos; Marí, B.; Ribelles, J.L. Gómez; Vidaurre, Ana

doi:10.1016/j.carbpol.2015.07.020

Cited by 30 publications

(14 citation statements)

References 32 publications

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“…What is clear is that besides by the DA, the crystallinity of chitosan membranes can be also highly influenced by the source of the material, the preparation or dissolving conditions and the drying procedure [47]. Indeed, in the case of the membranes analyzed in this experimental work, no structural differences were found as a function of the DA, as all formulations were in an amorphous state, proved by the presence of a diffuse XRD peak near 20°(2 Teta) and the absence of two intense peaks at 10°and 20° [48]. These results are in concordance with the literature, were it is described for chitosan membranes prepared by the solvent-casting technique, starting from an aqueous acetic acid solution, to be in an amorphous state.…”

Section: Discussionmentioning

confidence: 83%

Investigation of cell adhesion in chitosan membranes for peripheral nerve regeneration

Carvalho

López-Cebral

Silva‐Correia

et al. 2017

Materials Science and Engineering: C

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Peripheral nerve injuries have produced major concerns in regenerative medicine for several years, as the recovery of normal nerve function continues to be a significant clinical challenge. Chitosan (CHT), because of its good biocompatibility, biodegradability and physicochemical properties, has been widely used as a biomaterial in tissue engineering scaffolding. In this study, CHT membranes were produced with three different Degrees of Acetylation (DA), envisioning its application in peripheral nerve regeneration. The three CHT membranes (DA I: 1%, DA II: 2%, DA III: 5%) were extensively characterized and were found to have a smooth and flat surface, with DA III membrane having slightly higher roughness and surface energy. All the membranes presented suitable mechanical properties and did not show any signs of calcification after SBF test. Biodegradability was similar for all samples, and adequate to physically support neurite outgrowth. The in vitro cell culture results indicate selective cell adhesion. The CHT membranes favoured Schwann cells invasion and proliferation, with a display of appropriate cytoskeletal morphology. At the same time they presented low fibroblast infiltration. This fact may be greatly beneficial for the prevention of fibrotic tissue formation, a common phenomenon impairing peripheral nerve regeneration. The great deal of results obtained during this work permitted to select the formulation with the greatest potential for further biological tests.

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Section: Discussionmentioning

confidence: 83%

Investigation of cell adhesion in chitosan membranes for peripheral nerve regeneration

Carvalho

López-Cebral

Silva‐Correia

et al. 2017

Materials Science and Engineering: C

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“…The diffraction pattern of CmCHT showed two broad and poorly defined peaks around 2Ɵ≈13° and 2Ɵ≈25°. According to the literature, [36], [37] the Thermal properties were evaluated, by means of DSC experiments following the aforementioned conditions. In Figure 7, the second heating scan is represented for all polymer networks and also for pristine polymers.…”

Section: Miscibility and Phase Behavior Analyzed By X-ray Diffractionmentioning

confidence: 99%

Fast degrading polymer networks based on carboxymethyl chitosan

Gámiz-González

Guldris

Turpin

et al. 2017

Materials Today Communications

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In this work macroporous membrane for mesenchymal stem cells, MSCs, transplant has been developed. The membranes support cell seeding and proliferation and completely degrade in less than one week in "in vitro" culture. The biodegradable material is a polymer network based on carboxymethyl chitosan (a water soluble modification of chitosan) crosslinked by poly-(Ɛ-caprolactone) PCL, fragments which are susceptible to hydrolytic degradation. Synthesis was performed in solution in a common solvent for the two components of the network. The gel fraction was assessed by extraction in selective solvents. Physical characterization of networks of varying composition included water sorption capacity and the crystallinity of poly-(Ɛ-caprolactone) in the network. In this way polymer networks are synthesized that loose between 66 ± 5% and 89 ± 1% of their mass when immersed in water for 28 days. The same weight loss is attained in enzymatic medium in only 4 days. Porcine bone marrow MSCs were seeded in macroporous membranes to show cell viability, and proliferation up to 7 days culture when the biomaterial is completely dissolved in the medium.

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“…The films with the highest DDs presented the most intense peaks around 2θ=20-21 o , and the films with lower DDs showed the most intense peaks around 2θ=9-10 o [85]. The crystallinity was also dependent of the water content in hydrated or immersed chitosan films, showing that the water had an important role in the chitosan´s crystal structure [84,85].…”

Section: Crystallinitymentioning

confidence: 95%

“…The XRD and DSC experiments were carried out in order to obtain information about the miscibility and the crystallization of both components in the block-copolymer network. [82,140] with maxima at around 2θ≈11° and 2θ≈20° [85]. PCL has two intensive crystalline peaks around 2θ≈21° and 2θ≈23° [214].…”

Section: Xrd and Dsc Phase Separation And Crystallizationmentioning

confidence: 99%

“…The equilibrium water content, EWC, of the polymer networks obtained by Eq.5.1 is shown in [81,85], the CmCHT has a less ordered structure than CHT and presents two intense peaks around 2θ≈10° and 2θ≈20°. The substitution of the carboxymethyl group breaks the hydrogen bonds between polymer chains and thus decreasing crystallinity.…”

Section: Equilibrium Water Content (Ewc)mentioning

confidence: 99%

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Biodegradable hydrogels based on water-soluble chitosan for cell transplant

González¹,

Amparo²

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ATR-FTIR Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy aw Water activity CDCl3 Deuterated chloroform CG Collagen CHCl3 Chloroform CHT Chitosan CmCHT Carboxymethyl chitosan (COCl)2 Oxalyl chloride CP/MAS 13C-NMR Solid-state Cross-Polarization Magic Angle Spinning Carbon13-Nuclear Magnetic Resonance CS Chondroitin sulfate D2O Deuterium oxide DAPI 4´,6-Diamidino-2-Phenylindole DC Conversion degree of carboxymethyl chitosan DCl Deuterium chloride xiv DCM Dichloromethane DD Deacetylation degree DMEM

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Determining the influence of N-acetylation on water sorption in chitosan films

Cited by 30 publications

References 32 publications

Investigation of cell adhesion in chitosan membranes for peripheral nerve regeneration

Investigation of cell adhesion in chitosan membranes for peripheral nerve regeneration

Fast degrading polymer networks based on carboxymethyl chitosan

Biodegradable hydrogels based on water-soluble chitosan for cell transplant

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