Determining the elastic properties of aptamer-ricin single molecule multiple pathway interactions

Wang, Bin; Park, Bosoon; Kwon, Yong-Kuk; Xu, Bingqian

doi:10.1063/1.4876603

Cited by 5 publications

(2 citation statements)

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“…Other example are the three different stable binding conformations formed between ricin and anti-ricin aptamer (53). Selfinteractions of DaSP likely operate through both hydrogen bonds and sulfate-calcium bridges; however, the calciumand/or sulfate-free interactions mediated by hydrogen bonds are unable to effectively sustain cell adhesion in sponges, as demonstrated here in aggregation assays with desulfated derivatives and elsewhere, for more than one century, by dissociating sponge tissues in calcium-free seawater (54).…”

Section: Discussionmentioning

confidence: 96%

Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans

Vilanova

Santos

Aquino

et al. 2016

Journal of Biological Chemistry

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Early metazoans had to evolve the first cell adhesion mechanism addressed to maintain a distinctive multicellular morphology. As the oldest extant animals, sponges are good candidates for possessing remnants of the molecules responsible for this crucial evolutionary innovation. Cell adhesion in sponges is mediated by the calcium-dependent multivalent self-interactions of sulfated polysaccharides components of extracellular membrane-bound proteoglycans, namely aggregation factors. Here, we used atomic force microscopy to demonstrate that the aggregation factor of the sponge Desmapsamma anchorata has a circular supramolecular structure and that it thus belongs to the spongican family. Its sulfated polysaccharide units, which were characterized via nuclear magnetic resonance analysis, consist preponderantly of a central backbone composed of 3-␣-Glc1 units partially sulfated at 2-and 4-positions and branches of Pyr(4,6)␣-Gal133-␣-Fuc2(SO 3 )133-␣-Glc4(SO 3 )133-␣-Glc34-linked to the central ␣-Glc units. Single-molecule force measurements of self-binding forces of this sulfated polysaccharide and their chemically desulfated and carboxyl-reduced derivatives revealed that the sulfate epitopes and extracellular calcium are essential for providing the strength and stability necessary to sustain cell adhesion in sponges. We further discuss these findings within the framework of the role of molecular structures in the early evolution of metazoans.Molecular clock estimates, paleobiogeochemical evidence, and the most widely accepted phylogenetic inferences place sponges (phylum Porifera) at the root of the metazoan tree as the oldest extant multicellular animals (1-4). Such ancestry makes modern sponges suitable organisms for studying the cellular and molecular aspects of the evolution of multicellularity (5). A pivotal feature of metazoans is their cell adhesion molecular machinery, which directs the formation and maintenance of a distinctive multicellular morphology (6). In most animals, protein interactions, such as those in the cell junctions of epithelia, mediate cell adhesion (7); however, sponge multicellularity is sustained by a characteristic cell adhesion and recognition mechanism based on specific carbohydrate-carbohydrate interactions (8).Carbohydrates have vast structural plasticity, ubiquitous distribution in vertebrate and invertebrate tissues, and are commonly associated with the cell surface (9). Carbohydrate selfadhesion is maintained by relatively weak forces that can increase in orders of magnitude upon the multimerization of individual epitopes into polysaccharides (10). Some examples of specific carbohydrate self-interactions include the multivalent binding of Lewis x epitopes involved in the first steps of embryogenesis (11), glycolipid-glycolipid interactions controlling cell adhesion, spreading, and motility (12), and self-interactions of sulfated polysaccharides leading to species-specific cell adhesion in sponges (13).Proteoglycan-like molecules termed aggregation factors (AFs) 4 mediate intercellu...

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Section: Discussionmentioning

confidence: 96%

Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans

Vilanova

Santos

Aquino

et al. 2016

Journal of Biological Chemistry

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“…The force curves exhibited a clear, sudden peak during retraction [105]. Wang et al [106] examined the elastic properties of ricin and its interactions with anti-ricin aptamers, revealing distinct binding conformations with unique elastic properties. They developed a method to differentiate specific unbinding pathways by analyzing individual force-extension curves in a multi-pathway system.…”

Section: Unveiling the Mechanical Properties Of Cellsmentioning

confidence: 99%

Atomic Force Microscopy as a Tool to Study Transport Phenomena in Biological Systems

Kandapal

2023

Processes

Self Cite

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Biological interactions often involve the transport of molecules, ions, or other species across biological membranes or between interacting proteins. The understanding of these transport phenomena is crucial for the development of therapies for various diseases. Atomic force microscopy is a powerful tool that has been increasingly used to study biological systems at the nano scale. The high resolution, quantitative measurements, and the ability to probe biological interactions under near-physiological conditions make AFM an attractive tool for investigating transport phenomena in biological systems. In this article, we focus on the use of AFM in the study of the transport phenomena in biological systems. We discuss the principles of AFM, its instrumentation, and its application in the study of biomolecules and biological systems. We also provide a comprehensive overview of recent articles that have utilized AFM in the study of biomarkers in biological systems.

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Single-molecule detection of proteins and toxins in food using atomic force microscopy

Reese

2019

Trends in Food Science & Technology

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Determining the elastic properties of aptamer-ricin single molecule multiple pathway interactions

Abstract: Articles you may be interested inStudy of the interactions of proteins with a solid surface using complementary acoustic and optical techniques Biointerphases 9, 029015 (2014); 10.1116/1.4874736Elastic network normal modes provide a basis for protein structure refinement

Cited by 5 publications

References 29 publications

Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans

Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans

Atomic Force Microscopy as a Tool to Study Transport Phenomena in Biological Systems

Single-molecule detection of proteins and toxins in food using atomic force microscopy

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