Determining the Efficacy of Selenium Nanoparticles (Se-NPs) Conjugated with Zinc and Synthesized Using Aspergillus niger on some Immunological Parameters of Induced Immunosuppressed Rats

Abstract

This research was aimed to isolation and identification of Aspergillus niger from different location of garden in Tikrit university, to used in Se-NPs synthesis alone and conjugated with Zn, then its characterizes by detected the spectroscopy, shape and size of the nanoparticles, also determine the efficacy each of Se-NPs or Zn alone or after conjugated when orally dosage to laboratory rats that’s induced immunosuppressed by sandimmune on IgA, IgG and IL-6. The results were found that ability to isolated and identified the Aspergillus niger from different sites in the gardens of the Tikrit University. The synthesis of Se-NPs were obtained by converting sodium selenite (Na2SeO4) used the Aspergillus niger extract, also they obtained the Se-NPs conjugated with Zn. The confirm of synthesis were inferred through the color was changed to oranges. The value of these particles alone or their genera appeared at a wavelength of 262 and 269 nm, respectively. The scanning electron microscope (SEM) showed spherical particle shapes and sizes at 52.90 nm which were confirmed by X-ray diffraction. The orally dosage of Se-NPs for male laboratory rats alone or in combination induced immunusupressed affected the renormalization of IgA, IgG, and IL-6 t. Their normal levels of IgA at 205, 215 and 242 (mg/dL) and the IgG at 1019, 1046 and 1346 (mg/dL) while of IL-6 at 3.60, 4.13 and 6.63 (pmol/mL) respectively. These effect were refered the parameters to became equalized with their values in the group of control animals and returned to normal.