Determining Infected Aortic Aneurysm Treatment Using Focused Detection of <i>Helicobacter cinaedi</i>

Jien, Saito; Rimbara, Emiko; Shingo, Inaguma; Chihiro, Hasegawa; Kamiya, Satoshi; Mizuno, Akihiro; Sone, Yoshitsugu; Tatsuhito, Ogawa; Yukihide, Numata; Takahashi, Satoru; Miki, Asano

doi:10.3201/eid2807.212505

Cited by 3 publications

(4 citation statements)

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“…Later, the bacterium was found in other patient groups, including women and children ( Vandamme et al, 1990 ; Skirrow et al, 1993 ). Currently, both cases of H. cinaedi infection against a background of immunodeficiencies ( Saito et al, 2022 ; Roupie et al, 2023 ) and in immunocompetent patients have been reported ( Akiyama et al, 2021 ; Toyoshima et al, 2022 ).…”

Section: Pathologies Associated With the Bacterium Helicoba...mentioning

confidence: 99%

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Helicobacter cinaedi bacterium association with atherosclerosis and other diseases

Voronina,

Arapidi

2024

Front. Microbiol.

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Helicobacter is a genus of spiral-shaped Gram-negative enterohepatic bacteria whose members are capable of causing bacteremia in humans. One of the poorly studied members of this genus is the bacterium Helicobacter cinaedi. This microorganism was first isolated from human fecal samples in 1984. Although it was long considered to be associated with only immunocompromised patients, more evidence in recent years has implicated H. cinaedi in causing serious pathologies in immunocompetent populations. In addition, H. cinaedi is also reported to be associated with a few chronic or severe illnesses, such as atherosclerosis, which in turn can lead to the development of other cardiovascular pathologies: one of the leading causes of mortality worldwide. Helicobacter cinaedi often goes unnoticed in standard diagnostic methods due to its slow growth under microaerobic conditions. This often leads to significant underdetection and hence undermines the role of this bacterium in the pathogenesis of various diseases and the extent of its spread in humans. In this review, we have compiled information on pathologies associated with H. cinaedi, the occurrence of the bacterium in humans and animals, and the latest developments in diagnosing the bacterium and treating associated diseases.

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Section: Pathologies Associated With the Bacterium Helicoba...mentioning

confidence: 99%

“…This is the first project of a preventive strategy whose action is directed specifically at this microorganism. Today, treatment of patients infected with H. cinaedi is based on antibiotic therapy ( Lenskaya et al, 2022 ; Saito et al, 2022 ; Roupie et al, 2023 ).…”

Section: Pathologies Associated With the Bacterium Helicoba...mentioning

confidence: 99%

Helicobacter cinaedi bacterium association with atherosclerosis and other diseases

Voronina,

Arapidi

2024

Front. Microbiol.

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“…Helicobacter cinaedi is the most frequently isolated enterohepatic Helicobacter species strain from humans, mainly found in immunocompromised patients with bacteremia. H. cinaedi is responsible for recurrent cellulitis, infected aortic aneurysm, subdural hematoma, and hepatic cyst infection ( 2 – 6 ). H. cinaedi was first isolated in 1984 from a homosexual man with intestinal symptoms in Seattle, and it was first isolated in Japan in 2003 ( 7 , 8 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of HcaA, a novel autotransporter protein in Helicobacter cinaedi , and its role in host cell adhesion

Aoki,

Mori,

Matsui

et al. 2023

mSphere

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Helicobacter cinaedi infects the human gut and causes invasive infections such as bacteremia and cellulitis through bacterial translocation. However, the mechanism by which H. cinaedi attaches to host cells and establishes infection remains unclear. This study aimed to investigate the relationship between a novel putative autotransporter protein, H. cinaedi autotransporter protein A (HcaA), and its role in pathogenicity. The cytotoxicity of H. cinaedi infection in colon epithelial cell lines (Caco-2 and HT29) was assessed using a lactate dehydrogenase assay, and it was found that cytotoxicity significantly decreased upon HcaA knockout. Adhesion assays further revealed that the HcaA-knockout strain showed significantly reduced attachment to the human epithelial colorectal adenocarcinoma (Caco-2) cell line compared to that of the wild-type strain. Moreover, the recombinant HcaA protein demonstrated strong adhesion properties to the human monocytic cell line (U937). The adhesive activity was diminished when the Arg-Gly-Asp (RGD) motif in HcaA was replaced with RAD, indicating that the RGD motif in HcaA is crucial for host cell adhesion. To determine the role of HcaA in H. cinaedi infection in vivo , C57BL/6 mice were orally infected with wild-type and HcaA-knockout H. cinaedi strains. Bacterial colonization was assessed 7, 14, and 28 days post-infection. At 7 days post-infection, colonization was significantly lower in mice infected with the HcaA-knockout strain compared to those infected with the wild-type strain. In conclusion, our findings suggest that HcaA, a novel putative autotransporter protein in H. cinaedi , plays a significant role as an adhesin in establishing colonization. IMPORTANCE Helicobacter species are classified as gastric or enterohepatic according to their habitat. Among enterohepatic Helicobacter species, which inhabit the intestine, colon, and liver, Helicobacter cinaedi has been most frequently isolated from humans. H. cinaedi often causes bacteremia and cellulitis in immunocompromised hosts. Here, we focused on the H. cinaedi autotransporter protein A (HcaA), a novel virulence factor in H. cinaedi . We discovered that HcaA contributes to cell adhesion via its Arg-Gly-Asp motif. Furthermore, in animal experiments, bacterial colonization was reduced in mice infected with HcaA-knockout strains, supporting the hypothesis that HcaA contributes to H. cinaedi adhesion to host cells. Our study provides a novel mechanism for the establishment of H. cinaedi infections and provides new insights into the role of autotransporter proteins in the establishment of Helicobacter infection.

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“…H. cinaedi is the most frequently isolated enterohepatic Helicobacter species strain from humans, mainly found in immunocompromised patients with bacteremia. H. cinaedi is responsible for recurrent cellulitis, infected aortic aneurysm, subdural hematoma, and hepatic cyst infection (2)(3)(4)(5)(6). H. cinaedi was first isolated in 1984 from a homosexual man with intestinal symptoms in Seattle and it was first isolated in Japan in 2003 (7,8).…”

Section: Introductionmentioning

confidence: 99%

Characterization of HcaA, a novel autotransporter protein inHelicobacter cinaedi, and its role in host cell adhesion

Aoki

Mori

Matsui

et al. 2022

Preprint

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Helicobacter cinaediinfects the human gut and causes invasive infections such as bacteremia and cellulitis by bacterial translocation. However, howH. cinaediattaches to host cells and establishes infection has not been elucidated. In this study, we focused on a novel autotransporter protein, i.e.,H. cinaediautotransporter protein A (HcaA) to examine its relationship withH. cinaedipathogenicity. The cytotoxicity ofH. cinaediinfection in the colon epithelial cell lines (Caco-2 and HT-29) by lactate dehydrogenase assay showed a significant reduction in cytotoxicity by HcaA knockout. Adhesion assays further revealed that the HcaA-knockout strain showed significantly reduced attachment ofH. cinaedito the human epithelial colorectal adenocarcinoma cell line (Caco-2) compared to the wild-type strain. To determine the role of HcaA inH. cinaediinfection in vivo,H. cinaediwild-type and HcaA-knockout strains were orally infected C57BL/6 mice. The colonized bacteria were then measured 7, 14, and 28-days post-infection. The number of colonizedH. cinaedicells was significantly lower in HcaA-knockout strain infections than in wild-type strain infections at 7 days post-infection. Recombinant HcaA protein showed strong adhesion characteristics to the human monocytic cell line (U937) by adhesion assay used recombinant purified HcaA protein. The adherent activity was diminished by the replacement of the RGD motif in HcaA with RAD, indicating the contribution of the RGD motif in HcaA to host cell adherence. These results suggest that HcaA, a novel autotransporter protein inH. cinaedi, plays a significant role in establishing infection as an adhesin.

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Determining Infected Aortic Aneurysm Treatment Using Focused Detection of Helicobacter cinaedi

Cited by 3 publications

References 15 publications

Helicobacter cinaedi bacterium association with atherosclerosis and other diseases

Helicobacter cinaedi bacterium association with atherosclerosis and other diseases

Characterization of HcaA, a novel autotransporter protein in Helicobacter cinaedi , and its role in host cell adhesion

Characterization of HcaA, a novel autotransporter protein inHelicobacter cinaedi, and its role in host cell adhesion

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