Determining binding sites of drugs on human serum albumin using FIA-QCM

Zhang, Qundan; Huang, Yanyan; Zhao, Rui; Liu, Guoquan; Chen, Yi

doi:10.1016/j.bios.2008.03.009

Cited by 33 publications

(15 citation statements)

References 33 publications

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“…Other areas where QCM was used to detect alcohols, esters and terpenes are in the continuous surveillance of composting processes (Lieberzeit et al , 2008), volatile organic compounds in gas mixtures (Si et al , 2007) (Filippov et al , 2007), alcohols (Melegari et al , 2008) (Koshets et al , 2009), nonylphenol (Nabok et al ., 2007a), chloramphenicol (Adányi et al , 2006), T‐2 mycotoxin (Nabok et al ., 2007b), and paclitaxel (taxol), a natural anti‐cancer agent providing a promising alternative to classical analytical methods for a fast and easy determination of paclitaxel (Pastorino et al , 2006). QCM was also used to study the host–guest interaction between N,N‐dimethylformamide or cholic acid and a cyclodextrin derivative (Schofield et al , 2006), between heptaprenyl diphosphate synthase and the substrate farnesyl diphosphate (Suzuki et al , 2006), and to determine drug binding to human serum albumin in a competition binding assay with bilirubin using flow injection analysis (Zhang et al , 2008a). A QCM for low humidity detection was developed based on poly(l‐lactide) stabilized gold nanoparticles (Sun et al , 2007) and a hybrid humidity sensor was assembled using optical waveguides on a quartz crystal microbalance (Shinbo et al , 2009).…”

Section: Small Moleculesmentioning

confidence: 99%

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

Becker

Cooper

2011

J of Molecular Recognition

161

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Since the publication of the original review of piezoelectric acoustic sensors in this series there has been a consistent, gradual expansion in the number of published papers using 'quartz crystal microbalances' (QCM). Between 2001 and 2009, the number of QCM publications per annum has increased from 49 to 273, with a two-fold increase in papers per annum between 2004 and 2008. Within the field, comparing the time covered by the current to the previous review, there are trends towards increasing use of QCM in the study of protein adsorption to surfaces (93% increase), homeostasis (67% increase), protein-protein interactions (40% increase) and carbohydrates (43% increase). New commercial systems have been released that are driving the uptake of the technology for characterization of binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This paper highlights theoretical and practical aspects of the principles that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and membrane interfaces.

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Section: Small Moleculesmentioning

confidence: 99%

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

Becker

Cooper

2011

J of Molecular Recognition

161

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“…17) The microdialysis probe placed beside the BSA solution was perfused at relatively high rate, and the migrating rate of FP via the probe was regarded as the systemic absorption in this skin model. Because FP is a typical ligand for serum albumin site II, 18,19) the disposition of FP will be influenced by the existence of the plasma protein in the absorption process after topical application. 13) …”

mentioning

confidence: 99%

The Use of an Artificial Skin Model to Study Transdermal Absorption of Drugs in Inflamed Skin

Oshima

Suzuki

Yajima

et al. 2012

Biological & Pharmaceutical Bulletin

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Studies on drug disposition in inflamed skin are important for safe and effective application of topical drugs. Here, the absorption of flurbiprofen (FP) through inflamed skin was examined in vivo and in a skin-mimicking artificial model system. The model skin system consisted of a silicone membrane acting as a model stratum corneum, laminated dialysis membranes acting as a model of viable skin, and 2 microdialysis probes-one used for determination of FP concentration and one acting as a model vessel. This model system could be used for quantitative evaluation of complicated permeation processes. In the in vivo experiments, FP absorption was suppressed in rats with inflamed skin induced by an intracutaneous injection of a mixed solution of λ-carrageenan, zymosan, and casein. Bovine serum albumin solution was placed between the dialysis membranes in the model skin system to mimic protein leaching in skin; the results suggested that the delayed absorption of FP in inflamed skin was due to binding to serum proteins leaching in the tissue. Such a combination of in vivo experiments and a model skin system is useful for understanding complex phenomena in inflamed and damaged skin and reduces experimental animal use.Key words skin absorption; model skin system; inflamed skin; protein binding; flurbiprofen Although permeation through the stratum corneum is the rate-limiting step for transdermal absorption of drugs, the kinetics of drug transfer in the viable epidermis and dermis could influence the pharmacological effects of a drug systemically as well as locally. Prolonged retention of the drug in the epidermis could delay onset of the therapeutic effect and/or cause undesirable side effects in the local tissue. Binding of drugs to tissue components in the skin could relate to retention of the drugs. In the case of inflamed skin, the leaching of plasma proteins with drug-binding ability could also affect the disposition of the drugs in local tissue. 1,2) In the drug of low protein binding ratio, the effect of plasma proteins leached into the local tissue will be small, the drug of high protein binding ratio are conversely susceptible to leach of plasma proteins. Although most skin formulations are intended for sound skin without injury or inflammation, prolonged or repeated application can bring about skin inflammation; also, some topical preparations are applied specifically to treat skin with inflammation. Therefore, studies on drug disposition in inflamed skin are important for safe and effective application of topical drugs.Excised animal skin is usually used for in vitro permeation studies in the early stages of the formulation development of new products. However, in vitro experimental systems using such membranes are not suitable for evaluating the drug concentration profiles in skin because of the lack of local blood flow and physiological response.3-5) Various experimental designs, including in situ skin permeation experiments and in vitro isolated skin perfusion experiments, have been developed to study the d...

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“…The later technique allows label-free detection of the binding reaction between ligand and protein, and provides valuable quantitative information on the rate and equilibrium binding constants [19]. Quartz crystal microbalance (QCM) biosensors have been used in the rapid screening of possible interactions between small molecules and proteins [20][21][22][23]. The suitability of QCM for monitoring and assaying the interaction between antibody-antigen [20], drugs-proteins [21], receptor-ligand [22] as well as ligandprotein [6,23] has been confirmed.…”

Section: Introductionmentioning

confidence: 99%

Development of a sensitive method for selection of affinity ligand for trypsin using quartz crystal microbalance sensor

Bayramoğlu

Arıca

2011

Bioprocess Biosyst Eng

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In this work, a new methodology is developed for selection of affinity ligands towards the enzyme "trypsin" using quartz crystals microbalance (QCM) technique. To achieve this goal, the surface amination of gold plated QCM crystals was achieved in 13.56 MHz plasma polymerization system by using ethylenediamine. Three different ligands (i.e., 4-aminobenzamidine, 4-aminobenzoic acid, and phenylalanine) were immobilized on the aminated QCM crystals surface via glutaraldehyde coupling. All three ligand immobilized QCM crystals were characterized and compared under different experimental conditions. It was observed that the benzamidine ligand showed higher affinity to trypsin with a dissociation constant on the order of 1.76 × 10(-9) M, which is within the range of 10(-4)-10(-8) M for affinity ligands. Thus, its selectivity was suitable for purification of trypsin from biological fluids.

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Determining binding sites of drugs on human serum albumin using FIA-QCM

Cited by 33 publications

References 33 publications

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

The Use of an Artificial Skin Model to Study Transdermal Absorption of Drugs in Inflamed Skin

Development of a sensitive method for selection of affinity ligand for trypsin using quartz crystal microbalance sensor

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