Determined about sex: Sex-testing in 45 primate species using a 2Y/1X sex-typing assay

Cadamuro, Valérie Choesmel; Bouakaze, Caroline; Croze, Myriam; Schiavinato, Stéphanie; Tonasso, Laure; Gérard, Pascale; Fausser, Jean-Luc; Gibert, Morgane; Dugoujon, Jean‐Michel; Braga, José; Balaresque, Patricia

doi:10.1016/j.fsigen.2014.09.010

Cited by 9 publications

(9 citation statements)

References 25 publications

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“…The sex of the remains was determined by amplification of segments of the X- and Y-chromosomes (see Supplementary Methods and Supplementary Tables 1 and 2 ) 10 . The results confirmed the remains being those of a male individual, as also suggested by physical analysis of the bones.…”

Section: Resultsmentioning

confidence: 99%

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Identification of the remains of King Richard III

et al. 2014

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In 2012, a skeleton was excavated at the presumed site of the Grey Friars friary in Leicester, the last-known resting place of King Richard III. Archaeological, osteological and radiocarbon dating data were consistent with these being his remains. Here we report DNA analyses of both the skeletal remains and living relatives of Richard III. We find a perfect mitochondrial DNA match between the sequence obtained from the remains and one living relative, and a single-base substitution when compared with a second relative. Y-chromosome haplotypes from male-line relatives and the remains do not match, which could be attributed to a false-paternity event occurring in any of the intervening generations. DNA-predicted hair and eye colour are consistent with Richard’s appearance in an early portrait. We calculate likelihood ratios for the non-genetic and genetic data separately, and combined, and conclude that the evidence for the remains being those of Richard III is overwhelming.

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Section: Resultsmentioning

confidence: 99%

“…A newly designed sex-typing assay comprising of PCR primers for co-amplification of the SRY fragment with UTX and UTY homologous regions was used. This assay was designed to enable relatively small sized fragments of SRY, UTX and UTY to be co-amplified from samples likely to contain degraded DNA 10 .…”

Section: Methodsmentioning

confidence: 99%

Identification of the remains of King Richard III

et al. 2014

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“…Few studies describe processes for sex-typing in monkeys, apes and also gibbons by amplification of the SRY or/and AMEL gene (Di Fiore 2005;Cadamuro et al 2015). In our study, the amplification of SRY ran with great efficiency but in some cases we observed preferential amplification of the Y fragment.…”

Section: Discussionmentioning

confidence: 43%

“…Numerous studies describe procedures for sex-determination using the molecular approach (Bradley et al 2001;Di Fiore 2005;Cadamuro et al 2015) but for practical use it is more convenient to use one set of primers to determine sex broadly across the primates, to reach a simple and effective assay for a reasonable price.…”

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confidence: 99%

Sex determination in gibbons of genus Nomascus using non-invasive method

et al. 2016

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Gibbons of the genus Nomascus have a strong sexual dimorphism and dichromatism. As they mature, both sexes develop sex-specific pelage colour. In combination with physical similarities in the genitalia with both sexes, there are problems with determining the sex of young individuals compared to other genus of gibbons. This is a pilot study applying a multiplex polymerase chain reactions based on a non-invasive method for sex determination of gibbons. The study was conducted on 22 faecal samples from gibbons of the genus Nomascus. The animals were monitored by staff so that the samples were identified correctly and each sample was collected immediately after the defecation. Results confirmed the sex in all adult and juvenile animals with known sex; and 2 females and 5 males in juveniles were determined with unknown sex. The results of direct examination completely corresponded with the PCR results. The PCR reaction with template DNA isolated from faecal material required BSA usage, however, we observed the occurrence of nonspecific fragments. This did not affect the reliability of our results and we confirmed the usability of this method for this genus.

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“…Despite the co-deletion of PRKY , TBL1Y and PCDH11Y in the larger events (Jobling et al 2007), and reciprocal duplications (Murphy et al 2007; Wei et al 2015), no phenotypic effect has been described so far besides the aforementioned sex testing failure. Novel methods for sex testing have been proposed, which often integrate or replace the AMELX/Y test with UTX/Y , SRY or microsatellite typing (Cadamuro et al 2015; Santos et al 1998; Tozzo et al 2013). …”

Section: Targeted Y-cnv Studiesmentioning

confidence: 99%

Human Y chromosome copy number variation in the next generation sequencing era and beyond

Massaia

Xue

2017

Hum Genet

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The human Y chromosome provides a fertile ground for structural rearrangements owing to its haploidy and high content of repeated sequences. The methodologies used for copy number variation (CNV) studies have developed over the years. Low-throughput techniques based on direct observation of rearrangements were developed early on, and are still used, often to complement array-based or sequencing approaches which have limited power in regions with high repeat content and specifically in the presence of long, identical repeats, such as those found in human sex chromosomes. Some specific rearrangements have been investigated for decades; because of their effects on fertility, or their outstanding evolutionary features, the interest in these has not diminished. However, following the flourishing of large-scale genomics, several studies have investigated CNVs across the whole chromosome. These studies sometimes employ data generated within large genomic projects such as the DDD study or the 1000 Genomes Project, and often survey large samples of healthy individuals without any prior selection. Novel technologies based on sequencing long molecules and combinations of technologies, promise to stimulate the study of Y-CNVs in the immediate future.

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Determined about sex: Sex-testing in 45 primate species using a 2Y/1X sex-typing assay

Cited by 9 publications

References 25 publications

Identification of the remains of King Richard III

Identification of the remains of King Richard III

Sex determination in gibbons of genus Nomascus using non-invasive method

Human Y chromosome copy number variation in the next generation sequencing era and beyond

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